Journal of the Iranian Chemical Society

, Volume 15, Issue 7, pp 1561–1568 | Cite as

Surfactant-assisted dispersive liquid–liquid micro-extraction combined with magnetic solid-phase extraction for analysis of polyphenols in tobacco samples

  • Chunbo Liu
  • Zhihua Liu
  • Meng Wang
  • Yaling Yang
Original Paper


A novel and simple two-step micro-extraction technique combining surfactant-assisted dispersive liquid–liquid micro-extraction and magnetic solid-phase extraction prior to high-performance liquid chromatography was established for analysis of polyphenols including chlorogenic acid, caffeic acid, and scopoletin in tobacco samples. In the developed system, Fe3O4 nanoparticles were synthesized by a one-step chemical co-precipitation method and used to remove hydrophobic substances in tobacco samples by physical adsorption. Low-density solvent (1-heptanol) and cationic surfactant cethyltrimethyl ammonium bromide were employed as extraction solvent and disperser agent, respectively. Under the optimized experimental conditions, a good linearity of the method was obtained over the concentration range from 0.1 to 1000 ng mL−1 for target analytes. The limits of detection (S/N = 3) were 0.05 ng mL−1 for CGA, 0.10 ng mL−1 for CFA, and 0.12 ng mL−1 for SP, respectively. Finally, the applicability of the developed method was evaluated by extraction and determination of these three phenolic compounds in tobacco samples and satisfactory average recoveries of spiked samples were between 96.6 and 102.7%.


High-performance liquid chromatography Magnetic solid-phase extraction Polyphenols Surfactant-assisted dispersive liquid–liquid micro-extraction Tobacco 



The work was strongly supported by Science and Technology Project of Yunnan Province (No. 2016539200340124) and Analysis and Testing Foundation of Kunming University of Science and Technology.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

The paper does not contain any researches with human participants or animals performed by any of the authors.


  1. 1.
    L. Bazinet, Y. Degrandpré, A. Porter, Sep. Purif. Technol. 41, 101 (2005)CrossRefGoogle Scholar
  2. 2.
    K. Pyrzynska, M. Biesaga, TrAC Trend Anal. Chem. 28, 893 (2009)CrossRefGoogle Scholar
  3. 3.
    Z. Mao, R. Shan, J. Wang, W. Cai, X. Shao, Spectrochim. Acta A 128, 711 (2014)CrossRefGoogle Scholar
  4. 4.
    F.J. Li, Q. Liu, W.S. Cai, X.G. Shao, Chromatographia 69, 743 (2009)CrossRefGoogle Scholar
  5. 5.
    J. Gu, X. Zeng, B. Kong, Y. Mao, W. Liu, W. Wei, Chromatographia 71, 769 (2010)CrossRefGoogle Scholar
  6. 6.
    X. Gang, Y. Liu, Z. Xia, H.Y. Yang, Z.Y. Ren, M.M. Miao, Chromatographia 70, 1007 (2009)CrossRefGoogle Scholar
  7. 7.
    S.C. Moldoveanu, M. Kiser, J. Chromatogr. A 1141, 90 (2007)CrossRefPubMedGoogle Scholar
  8. 8.
    J. Wu, W.S. Rickert, A. Masters, J. Chromatogr. A 1264, 40 (2012)CrossRefPubMedGoogle Scholar
  9. 9.
    L. Nováková, Z. Spácil, M. Seifrtová, L. Opletal, P. Solich, Talanta 80, 1970 (2010)CrossRefPubMedGoogle Scholar
  10. 10.
    C. Özdemir, Ş. Saçmacı, Ş. Kartal, M. Saçmacı, J. Ind. Eng. Chem. 20, 4059 (2014)CrossRefGoogle Scholar
  11. 11.
    M. Wang, L. Wu, Q. Hu, Y. Yang, Environ. Sci. Pollut. Res. (2018). CrossRefGoogle Scholar
  12. 12.
    M. Moradi, Y. Yamini, A. Esrafili, S. Seidi, Talanta 82, 1864 (2010)CrossRefPubMedGoogle Scholar
  13. 13.
    Q. Deng, M. Chen, L. Kong, X. Zhao, J. Guo, X. Wen, Spectrochim. Acta A 104, 64 (2013)CrossRefGoogle Scholar
  14. 14.
    J. Vichapong, S. Srijaranai, Y. Santaladchaiyakit, W. Kanchanamayoon, R. Burakham, Food Anal. Methods 9, 1120 (2016)CrossRefGoogle Scholar
  15. 15.
    M. Behbahani, F. Najafi, S. Bagheri, M.K. Bojdi, M. Salarian, A. Bagheri, J. Chromatogr. A 1308, 25 (2013)CrossRefPubMedGoogle Scholar
  16. 16.
    X. Deng, Q. Guo, X. Chen, T. Xue, H. Wang, P. Yao, Food Chem. 145, 853 (2014)CrossRefPubMedGoogle Scholar
  17. 17.
    M. Hashemi, Z. Taherimaslak, S. Rashidi, Spectrochim. Acta A 128, 583 (2014)CrossRefGoogle Scholar
  18. 18.
    G. Morales-Cid, A. Fekete, B.M. Simonet, R. Lehmann, S. Cárdenas, X. Zhang, V. Miguel, P. Schmitt-Kopplin, Anal. Chem. 82, 2743 (2010)CrossRefPubMedGoogle Scholar
  19. 19.
    Y. Xu, J. Ding, H. Chen, Q. Zhao, J. Hou, J. Yan, H. Wang, L. Ding, N. Ren, Food Chem. 140, 83 (2013)CrossRefPubMedGoogle Scholar
  20. 20.
    S.D. Pan, X.H. Chen, H.Y. Shen, X.P. Li, M.Q. Cai, Y.G. Zhao, M.C. Jin, Anal. Chim. Acta 919, 34 (2016)CrossRefPubMedGoogle Scholar
  21. 21.
    M. Wang, Y. Jiao, C. Cheng, J. Hua, Y. Yang, Anal. Bioanal. Chem. 409, 7063 (2017)CrossRefPubMedGoogle Scholar
  22. 22.
    A.A. Asgharinezhad, N. Mollazadeh, H. Ebrahimzadeh, F. Mirbabaei, N. Shekari, J. Chromatogr. A 1338, 1 (2014)CrossRefPubMedGoogle Scholar
  23. 23.
    W. Liao, Y. Ma, A. Chen, Y. Yang, Chem. Eng. J. 271, 232 (2015)CrossRefGoogle Scholar
  24. 24.
    S. Ju, M. Liu, Y. Yang, Anal Lett. 49, 511 (2016)CrossRefGoogle Scholar
  25. 25.
    B. Ebrahimpour, Y. Yamini, M. Moradi, J Pharm. Biomed. 66, 264 (2012)CrossRefGoogle Scholar
  26. 26.
    M.S. Tehrani, M.H. Givianrad, N. Mahoor, Anal. Methods 4, 1357 (2012)CrossRefGoogle Scholar
  27. 27.
    S.M. Yousefi, F. Shemirani, J. Hazard. Mater. 254, 134 (2013)CrossRefPubMedGoogle Scholar
  28. 28.
    F. Xie, A. Yu, D. Hou, H. Liu, L. Ding, S. Zhang, Am. J. Anal. Chem. 2, 929 (2011)CrossRefGoogle Scholar
  29. 29.
    R. Zhao, F. Li, J. Hu, Anal. Methods 3, 2421 (2011)CrossRefGoogle Scholar
  30. 30.
    S. Saha, R. Mistri, B.C. Ray, Anal. Bioanal. Chem. 405, 9265 (2013)CrossRefPubMedGoogle Scholar
  31. 31.
    F. Xie, Y. Zhang, B. Zheng, F. Xu, J. Su, Y. Lu, F. Zeng, B. Zhang, Y. Guo, S. Zhang, Electrophoresis 33, 2433 (2012)CrossRefPubMedGoogle Scholar

Copyright information

© Iranian Chemical Society 2018

Authors and Affiliations

  • Chunbo Liu
    • 1
  • Zhihua Liu
    • 1
  • Meng Wang
    • 2
  • Yaling Yang
    • 2
  1. 1.Technology Center of Yunnan Tobacco Industry Limited Liability CompanyKunmingChina
  2. 2.Faculty of Life Science and TechnologyKunming University of Science and TechnologyKunmingChina

Personalised recommendations