Journal of the Iranian Chemical Society

, Volume 15, Issue 7, pp 1477–1483 | Cite as

Separation and determination of cinchona alkaloids by ligand-exchange capillary electrophoresis using a Cu(II)–l-lysine complex as selector

Original Paper
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Abstract

The purpose of this study was to develop a novel, simple and precise ligand-exchange capillary electrophoresis method to separate the diastereomer pairs of four major cinchona alkaloids (quinine/quinidine and cinchonine/cinchonidine). The l-lysine and copper(II) were chosen as the ligand and the central ion, respectively. Optimal separation conditions of four alkaloids were 4.0 mM l-lys, 2.0 mM copper(II) and 30 mM NH4Ac at pH 8.9 and a applied voltage of 15 kV . Meanwhile, the first-order derivative electropherogram was used for resolving the overlapping peaks of cinchonidine and quinidine. Under the optimum condition, good linearities were obtained with correlation coefficients from 0.9908 to 0.9935. The limits of detection (LOD, S/N = 3) and the limits of quantitation (LOQ, S/N = 10) ranged from 0.24 to 0.41 μg/mL and from 0.73 to 1.35 μg/mL, respectively. The recoveries ranged between 95.38 and 106.03%. The proposed ligand-exchange capillary electrophoresis method was suitable for the quantitative determination of four cinchona alkaloids in some preparations such as Compound Quinine Injection, Tonic Water and Klorane quinine and Vitamin B complex Shampoo.

Keywords

Ligand-exchange capillary electrophoresis First-order derivative electropherogram Cinchona alkaloids Simultaneous determination 

Abbreviation

LECE

Ligand-exchange capillary electrophoresis

QN

Quinine

QND

Quinidine

CN

Cinchonine

CND

Cinchonidine

l-lys

l-lysine

Cu(II)

Copper(II)

Rs

Resolution

Notes

Acknowledgements

This work was financially sponsored by the Natural Science Foundation of Shanxi Province (Nos. 201701D121141 and 201701D121023) and supported by the Fund for Shanxi Key Subject Construction (FSKSC2016).

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Copyright information

© Iranian Chemical Society 2018

Authors and Affiliations

  1. 1.College of PharmacyShanxi Medical UniversityTaiyuanChina

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