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Competitive binding affinity of two lanthanum(III) macrocycle complexes toward DNA and bovine serum albumin in water

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Abstract

In the present study, two water-soluble lanthanum(III) hexaaza Schiff base complexes were synthesized and characterized and also theoretically investigated. The interactions of these complexes with DNA and bovine serum albumin (BSA) were studied using different spectroscopic assessments and docking simulation analysis. The DNA docking studies suggested that these two complexes are able to interact with DNA through the minor groove, and also the binding affinity is in the order of La(L1) > La(L2). Furthermore, the spectral titration was carried out and viscosity measurements were taken. In this regard, protein-binding studies revealed that these complexes quench the intrinsic fluorescence of BSA, and indicated that the possible binding site is located on the vicinity of Trp 213, which is further validated by docking simulation analysis. The in vitro anticancer activities of these complexes indicated that the La(L1) complex is more effective than the other one and also exhibits a better interaction with DNA.

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Acknowledgements

We are grateful to Shiraz University Research Council for its financial support.

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Authors and Affiliations

  1. Department of Chemistry, College of Sciences, Shiraz University, Shiraz, 71454, Islamic Republic of Iran

    Zahra Asadi, Hamta Mosallaei & Moslem Sedaghat

  2. Protein Chemistry Laboratory (PCL), Department of Biology, Shiraz University, Shiraz, 71454, Islamic Republic of Iran

    Reza Yousefi

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  1. Zahra Asadi
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Correspondence to Zahra Asadi.

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Asadi, Z., Mosallaei, H., Sedaghat, M. et al. Competitive binding affinity of two lanthanum(III) macrocycle complexes toward DNA and bovine serum albumin in water. J IRAN CHEM SOC 14, 2367–2385 (2017). https://doi.org/10.1007/s13738-017-1172-3

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