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Simultaneous screening of homotaurine and taurine in marine macro-algae using liquid chromatography–fluorescence detection

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Abstract

Simultaneous analysis of homotaurine and its homologous, taurine, is a highly challenging issue, especially in matrices they exist simultaneously. A simple precolumn derivatization procedure combined with high-performance liquid chromatography–fluorescence detection was developed for simultaneous determination of homotaurine and taurine in marine macro-algae. The analytes were derivated with o-phthalaldehyde at an ambient temperature and alkaline medium. Calibration curves were linear in the ranges of 50–2500 µg L−1 for homotaurine and 100–2500 µg L−1 for taurine with the coefficients of determination higher than 0.998. Limits of detection of homotaurine and taurine were 15 and 30 µg L−1, respectively. Intraday (n = 6) and inter-day (n = 4) precisions of the method were satisfactory with relative standard deviations less than 6.0%. Good recoveries (>94%) were acquired by the method for extraction of homotaurine and taurine from algae matrices. Liquid chromatography–mass spectrometry was also used to confirm detection of the analytes in algae samples. The data suggest that the method was successfully applied to simultaneous determination of homotaurine and taurine in algae samples.

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Acknowledgements

The Iranian National Institute for Oceanography and Atmospheric Science is acknowledged for financially supporting the Project with Grant Number of 391–011–06.

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Authors and Affiliations

  1. Department of Marine Living Science, Ocean Sciences Research Center, Iranian National Institute for Oceanography and Atmospheric Science, Tehran, Iran

    Ali Mehdinia & Neda Sheijooni Fumani

  2. Department of Chemistry, Faculty of Science, K. N. Toosi University of Technology, Tehran, Iran

    Simindokht Rostami & Sahar Dadkhah

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  1. Ali Mehdinia
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  2. Simindokht Rostami
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  4. Neda Sheijooni Fumani
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Correspondence to Ali Mehdinia.

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Mehdinia, A., Rostami, S., Dadkhah, S. et al. Simultaneous screening of homotaurine and taurine in marine macro-algae using liquid chromatography–fluorescence detection. J IRAN CHEM SOC 14, 2135–2142 (2017). https://doi.org/10.1007/s13738-017-1150-9

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  • DOI: https://doi.org/10.1007/s13738-017-1150-9

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