Journal of the Iranian Chemical Society

, Volume 14, Issue 10, pp 2135–2142 | Cite as

Simultaneous screening of homotaurine and taurine in marine macro-algae using liquid chromatography–fluorescence detection

  • Ali MehdiniaEmail author
  • Simindokht Rostami
  • Sahar Dadkhah
  • Neda Sheijooni Fumani
Original Paper


Simultaneous analysis of homotaurine and its homologous, taurine, is a highly challenging issue, especially in matrices they exist simultaneously. A simple precolumn derivatization procedure combined with high-performance liquid chromatography–fluorescence detection was developed for simultaneous determination of homotaurine and taurine in marine macro-algae. The analytes were derivated with o-phthalaldehyde at an ambient temperature and alkaline medium. Calibration curves were linear in the ranges of 50–2500 µg L−1 for homotaurine and 100–2500 µg L−1 for taurine with the coefficients of determination higher than 0.998. Limits of detection of homotaurine and taurine were 15 and 30 µg L−1, respectively. Intraday (n = 6) and inter-day (n = 4) precisions of the method were satisfactory with relative standard deviations less than 6.0%. Good recoveries (>94%) were acquired by the method for extraction of homotaurine and taurine from algae matrices. Liquid chromatography–mass spectrometry was also used to confirm detection of the analytes in algae samples. The data suggest that the method was successfully applied to simultaneous determination of homotaurine and taurine in algae samples.


Homotaurine Taurine Precolumn derivatization HPLC Fluorescence detection Marine algae 



The Iranian National Institute for Oceanography and Atmospheric Science is acknowledged for financially supporting the Project with Grant Number of 391–011–06.


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Copyright information

© Iranian Chemical Society 2017

Authors and Affiliations

  • Ali Mehdinia
    • 1
    Email author
  • Simindokht Rostami
    • 2
  • Sahar Dadkhah
    • 2
  • Neda Sheijooni Fumani
    • 1
  1. 1.Department of Marine Living Science, Ocean Sciences Research CenterIranian National Institute for Oceanography and Atmospheric ScienceTehranIran
  2. 2.Department of Chemistry, Faculty of ScienceK. N. Toosi University of TechnologyTehranIran

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