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Fabrication of an ultrasensitive electrochemical sensor based on a mesoporous silica material functionalized by copper ion (SBA-15-Cu(II)) modified carbon paste electrode for determination of antibiotic ceftazidime and its application in pharmaceutical and biological samples

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A simple, sensitive and selective sensor based on carbon paste electrode modified by functionalized mesoporous silica material was developed for the electrochemical determination of ceftazidime (CFZ). The functionalized mesoporous silica was synthesized through a self-assembly process using triblock copolymer as template under acidic conditions and then incorporated in the carbon paste electrode as a modifier. This modifier was characterized by transmission electron microscopy, X-ray diffraction and scanning electron microscopy. The oxidation of CFZ was studied on modified carbon paste electrode using cyclic voltammetry, differential pulse voltammetry and electrochemical impedance spectroscopy (EIS). The results show that the oxidation peak current of CFZ on the modified carbon paste electrode was significantly improved compared to that obtained on the bare carbon paste electrode. Under optimum conditions, the sensor exhibited a linear response over the CFZ concentration range of 1–2500 nM, with a detection limit of 0.3 nM. The proposed sensor was successfully applied for monitoring of CFZ in the pharmaceutical and biological samples, and satisfactory results were obtained.

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Carbon paste electrode


X-ray diffraction


Scanning electron microscopy


Transmission electron microscopy


Cyclic voltammetric


Differential pulse voltammetric


Electrochemical impedance spectroscopy


  1. 1.

    t.e. The Merck Index, Merck, Rahway, N.J. (1983) p. 1913

  2. 2.

    K. Florey, Ceftazidime, analytical profiles of drug substances, vol. 19 (Academic Press, New York, 1990)

  3. 3.

    I.M.A.B.M. Drug Information 89. American Society of Hospital in Pharmacists, in Analytical Profiles of DrugSubstances, vol. 19, 1990, p. 111

  4. 4.

    C.D. Farrell, F.J. Rowell, R.H. Cumming, A rapid fluorescence ELISA for ceftazidime, Analytical Proceedings including Analytical Communications, Royal Society of Chemistry 1995, pp. 205–206

  5. 5.

    K. Arun, R. Saravanan, M. Balachandar, B. Kumuthavalli, B. Jayakar, UV-Spectrophotometric determination of ceftazidime in pure and pharmaceutical formulation. J. Chem. Pharm. Res. 2, 424–431 (2010)

  6. 6.

    B. Hiremath, B.H. Mruthyunjayaswamy, Development and validation of spectrophotometric methods for determination of ceftazidime in pharmaceutical dosage forms. Acta Pharm 58, 275–285 (2008)

  7. 7.

    S. Mahramyari, E. Pourbasheer, A. Banaei, M.R. Ganjali, P. Norouzi, Simultaneous spectrophotometric determination of ceftazidime and sulbactam using multivariate calibration methods. RSC Adv 4, 41039–41044 (2014)

  8. 8.

    A.D.H. Moreno, H.R.N. Salgado, Spectrophotometric determination of ceftazidime in pharmaceutical preparations using neocuproin as a complexing agent. Anal. Lett. 41, 2143–2152 (2008)

  9. 9.

    G. Ye, X. Cai, B. Wang, Z. Zhou, X. Yu, W. Wang et al., Simultaneous determination of vancomycin and ceftazidime in cerebrospinal fluid in craniotomy patients by high-performance liquid chromatography. J. Pharm. Biomed. Anal. 48, 860–865 (2008)

  10. 10.

    A. de Haro Moreno, H.R.N. Salgado, Development of a new high-performance liquid chromatographic method for the determination of ceftazidime. J. AOAC Int. 91, 739–743 (2008)

  11. 11.

    A. Isla, A. Arzuaga, J. Maynar, A. Gascon, M. Solinis, E. Corral et al., Determination of ceftazidime and cefepime in plasma and dialysate-ultrafiltrate from patients undergoing continuous veno-venous hemodiafiltration by HPLC. J. Pharm. Biomed. Anal. 39, 996–1005 (2005)

  12. 12.

    W. Xu, P. Gao, X. Zhang, L. Song, Evaluation of Ceftazidime contents in antibiotic discs by capillary electrophoresis. New Microbiol. 31, 377 (2008)

  13. 13.

    R. Wang, L. Chen, Z. Jia, J. Xie, X. Hu, Y. Li, Simultaneous enantiomeric separation of ceftazidime and cefriaxone sodium by capillary electrophoresis. Chin. J. Anal. Chem. 30, 1070–1073 (2002)

  14. 14.

    G. Deby-Dupont, C. Deby, A. Mouithys-Mickalad, M. Hoebeke, M. Mathy-Hartert, L. Jadoul et al., The antibiotic ceftazidime is a singlet oxygen quencher as demonstrated by ultra-weak chemiluminescence and by inhibition of AAP consumption. Biochim. Biophys. Acta 1379, 61–68 (1998)

  15. 15.

    D. Tarinc, B. Dogan-Topal, A. Golcu, S.A. Ozkan, Electrochemical investigation and determination of ceftazidime in pharmaceutical dosage forms and human urine. J. Anal. Chem. 69, 899–908 (2014)

  16. 16.

    B. Dogan, B. Uslu, S.A. Ozkan, P. Zuman, Electrochemical determination of HIV drug abacavir based on its reduction. Anal. Chem. 80, 209–216 (2008)

  17. 17.

    Ş. Demircan, S. Kır, S. Ozkan, Electroanalytical characterization of verapamil and its voltammetric determination in pharmaceuticals and human serum. Anal. Lett. 40, 1177–1195 (2007)

  18. 18.

    N.A. El-Maali, Voltammetric analysis of ceftazidime after preconcentration at various mercury and carbon electrodes: application to sub-ppb level determination in urine samples. Talanta 51, 957–968 (2000)

  19. 19.

    V.S. Ferreira, M.V.B. Zanoni, M. Furlan, A.G. Fogg, Differential pulse polarographic determination of ceftazidime in urine samples with and without prior extraction. Anal. Chim. Acta 351, 105–114 (1997)

  20. 20.

    V.S. Ferreira, M.V.B. Zanoni, A.G. Fogg, Cathodic stripping voltammetric determination of ceftazidime in urine at a hanging mercury drop electrode. Microchem. J. 57, 115–122 (1997)

  21. 21.

    V.S. Ferreira, M.V.B. Zanoni, A.G. Fogg, Indirect cathodic-stripping voltammetric determination of ceftazidime as a mercury salt. Anal. Chim. Acta 367, 255–259 (1998)

  22. 22.

    V.S. Ferreira, M.V.B. Zanoni, A.G. Fogg, Cathodic stripping voltammetric determination of ceftazidime with reactive accumulation at a poly-l-lysine modified hanging mercury drop electrode. Anal. Chim. Acta 384, 159–166 (1999)

  23. 23.

    M.M. Aleksić, N. Lijeskić, J. Pantić, V.P. Kapetanović, Electrochemical behavior and differential pulse voltammetric determination of ceftazidime, cefuroxime-axetil and ceftriaxone. Facta Univ. Ser. Phys. Chem. Technol. 11, 55–66 (2013)

  24. 24.

    S. Shahrokhian, R. Salimian, S. Rastgar, Pd–Au nanoparticle decorated carbon nanotube as a sensing layer on the surface of glassy carbon electrode for electrochemical determination of ceftazidime. Mater. Sci. Eng., C 34, 318–325 (2014)

  25. 25.

    S. Fathi, A novel and low cost electrochemical sensor for ceftazidime and cefazoline as antibiotic drugs based on nickel/SDS-poly (o-aminophenol) modified electrode. Russ. J. Electrochem. 50, 468–475 (2014)

  26. 26.

    M. Torkashvand, M. Gholivand, G. Malekzadeh, Construction of a new electrochemical sensor based on molecular imprinting recognition sites on multiwall carbon nanotube surface for analysis of ceftazidime in real samples. Sens. Actuators B Chem. 231, 759–767 (2016)

  27. 27.

    M.B. Gholivand, M. Amiri, Simultaneous detection of dopamine and acetaminophen by modified gold electrode with polypyrrole/aszophloxine film. J. Electroanal. Chem. 676, 53–59 (2012)

  28. 28.

    M.B. Gholivand, M. Amiri, Highly sensitive and selective determination methyldopa in the presence of ascorbic acid using OPPy/TY/Au modified electrode. J. Electroanal. Chem. 694, 56–60 (2013)

  29. 29.

    M. Gholivand, N. Karimian, Fabrication of a highly selective and sensitive voltammetric ganciclovir sensor based on electropolymerized molecularly imprinted polymer and gold nanoparticles on multiwall carbon nanotubes/glassy carbon electrode. Sens. Actuators B Chem. 215, 471–479 (2015)

  30. 30.

    M.B. Gholivand, L. Mohammadi-Behzad, Fabrication of a highly sensitive sumatriptan sensor based on ultrasonic-electrodeposition of Pt nanoparticles on the ZrO 2 nanoparticles modified carbon paste electrode. J. Electroanal. Chem. 712, 33–39 (2014)

  31. 31.

    M.B. Gholivand, M. Torkashvand, Electrooxidation behavior of warfarin in Fe 3 O 4 nanoparticles modified carbon paste electrode and its determination in real samples. Mater. Sci. Eng., C 48, 235–242 (2015)

  32. 32.

    J. Ortiz-Bustos, A. Martín, V. Morales, R. Sanz, R. García-Muñoz, Surface-functionalization of mesoporous SBA-15 silica materials for controlled release of methylprednisolone sodium hemisuccinate: Influence of functionality type and strategies of incorporation. Microporous and Mesoporous Materials, (2016)

  33. 33.

    E. Da’na, A. Sayari, Adsorption of heavy metals on amine-functionalized SBA-15 prepared by co-condensation: applications to real water samples. Desalination 285, 62–67 (2012)

  34. 34.

    S.-W. Song, K. Hidajat, S. Kawi, Functionalized SBA-15 materials as carriers for controlled drug delivery: influence of surface properties on matrix–drug interactions. Langmuir 21, 9568–9575 (2005)

  35. 35.

    S. Dehdashtian, M.B. Gholivand, M. Shamsipur, Z. Karimi, A nano sized functionalized mesoporous silica modified carbon paste electrode as a novel, simple, robust and selective anti-diabetic metformin sensor. Sens. Actuators B Chem. 221, 807–815 (2015)

  36. 36.

    D. Zhao, J. Feng, Q. Huo, N. Melosh, G.H. Fredrickson, B.F. Chmelka et al., Triblock copolymer syntheses of mesoporous silica with periodic 50–300 angstrom pores. Science 279, 548–552 (1998)

  37. 37.

    Y. Jiang, Q. Gao, H. Yu, Y. Chen, F. Deng, Intensively competitive adsorption for heavy metal ions by PAMAM-SBA-15 and EDTA-PAMAM-SBA-15 inorganic–organic hybrid materials. Microporous Mesoporous Mater. 103, 316–324 (2007)

  38. 38.

    N.A. El-Maali, A. Osman, A. Aly, G. Al-Hazmi, Voltammetric analysis of Cu (II), Cd (II) and Zn (II) complexes and their cyclic voltammetry with several cephalosporin antibiotics. Bioelectrochemistry 65, 95–104 (2005)

  39. 39.

    E. Laviron, General expression of the linear potential sweep voltammogram in the case of diffusionless electrochemical systems. J. Electroanal. Chem. Interfacial Electrochem. 101, 19–28 (1979)

  40. 40.

    P. Schmuki, Pits and Pores II: Formation, Properties, and Significance for Advanced Materials: Proceedings of the International Symposium, The Electrochemical Society 2001

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Correspondence to Sara Dehdashtian.

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Dehdashtian, S., Abdipur, Z. Fabrication of an ultrasensitive electrochemical sensor based on a mesoporous silica material functionalized by copper ion (SBA-15-Cu(II)) modified carbon paste electrode for determination of antibiotic ceftazidime and its application in pharmaceutical and biological samples. J IRAN CHEM SOC 14, 1699–1709 (2017). https://doi.org/10.1007/s13738-017-1111-3

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  • Ceftazidime
  • Mesoporous silica
  • Cyclic voltammetry
  • Electrochemical sensor