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Supramolecular solvent-based microextraction of warfarin from biological samples and its determination using HPLC

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Abstract

Extraction and determination of warfarin were performed using supramolecular solvent-based microextraction (SSME) and high performance liquid chromatography (HPLC). Effective factors on SSME including pH and temperature of sample, stirring speed, volume of the solvent, salt effect, and extraction time were investigated and optimized. Determination of warfarin was performed by HPLC using a mobile phase consisting of methanol and (0.5 %) phosphoric acid solution (80:20 v/v) and UV detector set at 320 nm. Under the optimized conditions pH of sample solution = 3, sample temperature = 30 °C, stirring speed = 800 rpm, volume of the solvent = 30 µL, without salt addition and extraction time = 30 min were obtained. The limit of detection was in the range of 0.4–14.5 ng mL−1. Linearity of the method was determined in the range of 14.5–300.0 ng mL−1 for plasma and 2.9–300.0 ng mL−1 for urine with coefficient of determination (R 2) ranging from 0.9975 to 0.9985. The method was applied successfully for determination of warfarin in plasma and urine samples.

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References

  1. R. Parkash, V. Wee, M.J. Gardner, J.L. Cox, K. Thompson, B. Brownell, D.R. Anderson, Can. J. Cardiol. 23, 457 (2007)

    Article  CAS  Google Scholar 

  2. G.W. Albers, J.E. Dalen, A. Laupacis, W.J. Manning, P. Petersen, D.E. Singer, Chest 119, 194S (2001)

    Article  CAS  Google Scholar 

  3. S. Sun, M. Wang, L. Su, J. Li, H. Li, D. Gu, J. Pharm. Biomed. Anal. 42, 218 (2006)

    Article  CAS  Google Scholar 

  4. W. Ju, K. Peng, S. Yang, H. Sun, M. Sampson, M.Z. Wang, Austin J. Anal. Pharm. Chem. 1, 8 (2014)

    Google Scholar 

  5. M.A. Radwan, G.A. Bawazeer, N.M. Aloudah, B.T. AlQuadeib, H.Y. Aboul-Enein, Biomed. Chromatogr. 26, 6 (2012)

    Article  CAS  Google Scholar 

  6. ​Technical report series 48th report, WHO Expert Committee on Biological Standardization. Guidelines for thrombo-plastins and plasma used to control oral anticoagulant therapy. Geneva, Switzerland, p 889 (1999)

  7. R. Lombardi, V. Chantarangkul, M. Cattaneo, A. Tripodi, Thromb. Res. 111, 281 (2003)

    Article  CAS  Google Scholar 

  8. C. Huang, J. Yang, Y. Du, L. Miao, Clin. Chim. Acta 393, 85 (2008)

    Article  CAS  Google Scholar 

  9. A. Osman, K. Arbring, T.L. Lindahl, J. Chromatogr. B 826, 75 (2005)

    Article  CAS  Google Scholar 

  10. I. Locatelli, V. Kmetec, A. Mrhar, I. Grabnar, J. Chromatogr. B 818, 191 (2005)

    Article  CAS  Google Scholar 

  11. P.R. Ring, J.M. Bostick, J. Pharm. Biomed. Anal. 22, 573 (2000)

    Article  CAS  Google Scholar 

  12. K.R. Henne, A. Gaedigk, G. Gupta, J.S. Leeder, A.E. Rettie, J. Chromatogr. B 710, 143 (1998)

    Article  CAS  Google Scholar 

  13. V.K. Boppana, W.H. Schaefer, M.J. Cyronak, J. Biochem. Biophys. Methods 54, 315 (2002)

    Article  CAS  Google Scholar 

  14. J. X. de Vries, E. Schmitz- Kummer, J. Chromatogr. A 644, 315 (1993)

  15. S.D. McAleer, H. Chrystyn, A.S. Foondun, Chirality 4, 488 (1992)

    Article  CAS  Google Scholar 

  16. C. Banfield, M. Rowland, J. Pharm. Sci. 73, 1392 (1984)

    Article  CAS  Google Scholar 

  17. Y.A. Chua, W.Z. Abdullah, S.H. Gun, Turk. J. Med. Sci. 42, 930 (2012)

    CAS  Google Scholar 

  18. Z. Zuo, S.K. Wo, C.M.Y. Lo, L. Zhou, G. Cheng, J.H.S. You, J. Pharm. Biomed. Anal. 52, 305 (2010)

    Article  CAS  Google Scholar 

  19. W. Naidong, P.R. Ring, C. Midtlien, X. Jiang, J. Pharm. Biomed. Anal. 25, 219 (2001)

    Article  CAS  Google Scholar 

  20. Z. Zhang, B.M. King, Y.N. Wong, Anal. Biochem. 298, 40 (2001)

    Article  CAS  Google Scholar 

  21. J. Hou, J. Zheng, S.A. Shamsi, J. Chromatogr. A 1159, 208 (2007)

    Article  CAS  Google Scholar 

  22. R.A. Coe, J.O. Rathe, J.W. Lee, J. Pharm. Biomed. Anal. 42, 573 (2006)

    Article  CAS  Google Scholar 

  23. W. Yau, E. Chan, J. Pharm. Biomed. Anal. 28, 107 (2002)

    Article  CAS  Google Scholar 

  24. P. Gareil, J.P. Gramond, F. Guyon, J. Chromatogr. B 615, 317 (1993)

    Article  CAS  Google Scholar 

  25. M. Balchen, A. Gjelstad, K.E. Rasmussen, S. Pedersen-Bjergaard, J. Chromatogr. A 1152, 220 (2007)

    Article  CAS  Google Scholar 

  26. M.M. Ghoneim, A. Tawfik, Anal. Chim. Acta 511, 63 (2004)

    Article  CAS  Google Scholar 

  27. F. Ruiz, S. Rubio, D. Pérez-Bendito, Anal. Chem. 78, 7229 (2006)

    Article  CAS  Google Scholar 

  28. N. Caballero-Casero, S. García-Fonseca, S. Rubio, Talanta 89, 377 (2012)

    Article  CAS  Google Scholar 

  29. M. Moradi, Y. Yamini, J. Sep. Sci. 35, 2319 (2012)

    Article  CAS  Google Scholar 

  30. A. Ballesteros-Gómez, M. Dolores Sicilia, S. Rubio, Anal. Chim. Acta 677, 108 (2010)

    Article  Google Scholar 

  31. F.J. López-Jiménez, S. Rubio, D. Pérez-Bendito, J. Chromatogr. A 1195, 25 (2008)

    Article  Google Scholar 

  32. M. Moradi, Y. Yamini, J. Chromatogr. A 1229, 30 (2012)

    Article  CAS  Google Scholar 

  33. S. Jafarvand, F. Shemirani, J. Sep. Sci. 34, 455 (2011)

    Article  CAS  Google Scholar 

  34. M.R. Hadjmohammadi, H. Ghambari, J. Pharm. Biomed. Anal. 61, 44 (2012)

    Article  CAS  Google Scholar 

  35. F. Rezaei, Y. Yamini, M. Moradi, B. Daraei, Anal. Chim. Acta 804, 135 (2013)

    Article  CAS  Google Scholar 

  36. E. Tahmasebi, Y. Yamini, A. Saleh, J. Chromatogr. B 877, 1923 (2009)

    Article  CAS  Google Scholar 

  37. D. Nagaraju, S. Huang, J. Chromatogr. A 1161, 89 (2007)

    Article  CAS  Google Scholar 

  38. E. Psillakis, N. Kalogerakis, Trends Anal. Chem. 22, 565 (2003)

    Article  CAS  Google Scholar 

  39. M.R. Hadjmohammadi, H. Ghambari, J. Chromatogr. B 899, 66 (2012)

    Article  Google Scholar 

  40. M. Jin, X. Chen, H. Chen, J. Liq. Chromatogr. Relat Technol. 29, 2641 (2006)

    Article  CAS  Google Scholar 

  41. M. Moradi, Y. Yamini, F. Rezaei, E. Tahmasebi, A. Esrafili, Analyst 137, 3549 (2012)

    Article  CAS  Google Scholar 

Download references

Conflict of interest

Mohammadreza Hadjmohammadi declares that he has no conflict of interest. Moazameh Payrovi declares that she has no conflict of interest.

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Authors and Affiliations

  1. Department of Chemistry, University of Mazandaran, NirooHavayi Boulevard, 47416-95447, Babolsar, Iran

    Moazameh Peyrovi & Mohammadreza Hadjmohammadi

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  1. Moazameh Peyrovi
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  2. Mohammadreza Hadjmohammadi
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Correspondence to Mohammadreza Hadjmohammadi.

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Peyrovi, M., Hadjmohammadi, M. Supramolecular solvent-based microextraction of warfarin from biological samples and its determination using HPLC. J IRAN CHEM SOC 12, 1253–1259 (2015). https://doi.org/10.1007/s13738-015-0589-9

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  • DOI: https://doi.org/10.1007/s13738-015-0589-9

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