Abstract
In this research, a simple method was developed for the separation and determination of l-tryptophan in aqueous solutions. The method consists of a solid-phase extraction (SPE) cleanup system utilizing a column packed with nickel–aluminum layered double hydroxide as a nano-sorbent, followed by a spectrofluorometric detection step. Some factors influencing the SPE efficiency, including pH, the sample flow rate, the elution conditions and the amount of nano-sorbent were evaluated and optimized. The selectivity of the presented SPE method with regard to l-tryptophan was studied in the presence of other amino acids and potentially interfering ions. Under optimum conditions, the calibration graph was linear in the range of 0.1–120.0 µg L−1 with a correlation coefficient of 0.9975. The limit of detection and relative standard deviation for l-tryptophan were 0.01 µg L−1 and 0.62 %, respectively. The maximum sorption capacity of the sorbent for l-tryptophan was 37.6 mg g−1. The method was successfully applied to determine l-tryptophan in several food samples with recoveries in the range of 96.2–104.2 % for the spiked samples.
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The financial support from the Research Council of Azarbaijan Shahid Madani University (ASMU, Iran (Grant no. 10799)) is gratefully acknowledged.
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Hossein Abdolmohammad-Zadeh declares that he has no conflict of interest. Seyed Morteza Hammami Oskooyi declares that he has no conflict of interest.
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Abdolmohammad-Zadeh, H., Hammami Oskooyi, S.M. Solid-phase extraction of l-tryptophan from food samples utilizing a layered double hydroxide nano-sorbent prior to its determination by spectrofluorometry. J IRAN CHEM SOC 12, 1115–1122 (2015). https://doi.org/10.1007/s13738-014-0572-x
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DOI: https://doi.org/10.1007/s13738-014-0572-x