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Microperoxidase-11/NH2-FSM16 as a H2O2-resistant heterogeneous nanobiocatalyst: a suicide-inactivation study

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Abstract

The catalytic activity of heme peptides is an area of intense investigation. They are utilized for exploring the fine details of structural and functional properties of an active site, and to create minimized and industrial catalysts. The peroxidase activity and kinetics of suicide-inactivation of microperoxidase-11/FSM16 as a heterogeneous nanobiocatalyst in oxidation reaction of guaiacol were studied in the presence of high concentration of hydrogen peroxide (2 mM), as its natural suicide-substrate. The substrate concentration was first-order in relation to aromatic substrate (AH), and the ratio of suicide-substrate (H2O2) was kept much higher than the benign substrate (guaiacol). The results of kinetic analysis confirmed a similar mechanism for suicide-peroxide inactivation of horseradish peroxidase (HRP), microperoxidase (MP-11) and MP-11/NH2-FSM16. Inactivation kinetic parameters, including intact activity of MP-11/NH2-FSM16, αi, and the apparent inactivation rate constant (k i) were obtained as 0.229 ± 0.009 min−1 and 0.651 ± 0.041 min−1 at [H2O2] = 2.0 mM, respectively, in 5.0 mM phosphate buffer solution (PBS; pH 7.0) at 27 °C. Our results indicated that covalent immobilization of microperoxidase onto NH2-FSM16 protected the heme group against peroxide inactivation resulting in generation of an efficient peroxide-resistant heterogeneous nanobiocatalyst.

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Acknowledgments

The financial support of Research Council of the University of Tehran and Iran National Science Foundation (INSF) is gratefully acknowledged.

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Authors and Affiliations

  1. Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran

    Yahya Sefidbakht, Farzaneh Farivar, Zainab Moosavi-Movahedi & Ali Akbar Moosavi-Movahedi

  2. Research Institute of Petroleum Industry, N.I.O.C, Tehran, Iran

    Khodadad Nazari

  3. Department of Ophthalmology and Visual Sciences, University of Wisconsin School of Medicine and Public Health, Madison, WI, 53792, USA

    Nader Sheibani

  4. Department of Pharmacology, University of Wisconsin School of Medicine and Public Health, Madison, USA

    Nader Sheibani

  5. Center of Excellence in Biothermodynamics, IBB, University of Tehran, Tehran, Iran

    Ali Akbar Moosavi-Movahedi

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  1. Yahya Sefidbakht
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Correspondence to Ali Akbar Moosavi-Movahedi.

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Sefidbakht, Y., Nazari, K., Farivar, F. et al. Microperoxidase-11/NH2-FSM16 as a H2O2-resistant heterogeneous nanobiocatalyst: a suicide-inactivation study. J IRAN CHEM SOC 9, 121–128 (2012). https://doi.org/10.1007/s13738-011-0040-9

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