Skip to main content
SpringerLink
Log in

Construction of dsDNA biosensor based on dendritic SiO2 nanoparticle and investigation on its interaction with benzo[a]pyrene

  • Original Paper
  • Published:
Journal of the Iranian Chemical Society Aims and scope Submit manuscript

Abstract

In this work, a new dsDNA biosensor was constructed to monitor the interaction of DNA and benzo[a]pyrene (BaP). Firstly, dendritic SiO2 nanoparticles were synthesized by silanization of SiO2 nanoparticles with (γ-(methacryloyloxy)propyl) trimethoxysilane and polymerization with acrylic acid. Then, due to the rich carboxyl groups of these nanoparticles, they were associated with the amino groups of a self-assembly membrane formed on the gold electrode by a sulfur-containing compound, 5-amino-3-mercapto-1,2,4-triazole. Finally, dsDNA was immobilized on the electrode surface by static adsorption with the aid of metallic ion. The whole immobilization steps were characterized by cyclic voltammogram and electrochemical impedance spectrum. After that, using methylene blue as a probe, the interaction of BaP with dsDNA was investigated. A linear relationship between the percentages of current decrease with the logarithm of BaP concentrations was found in the range from 0.33 to 133 μM.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Scheme 1
Scheme 2
Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8

Similar content being viewed by others

References

  1. X. Lin, H. Wan, Y. Zhang, J.H. Chen, Talanta 74, 944 (2008)

    Article  CAS  Google Scholar 

  2. M. Ravera, G. Bagni, M. Mascini, J.C. Dabrowiak, D. Osella, J. Inorg. Biochem. 101, 1023 (2007)

    Article  CAS  Google Scholar 

  3. S.S. Babkina, N.A. Ulakhovich, Bioelectrochemistry 63, 261 (2004)

    Article  CAS  Google Scholar 

  4. X.D. Su, Y.J. Wu, W. Knoll, Biosens. Bioelectron. 21, 719 (2005)

    Article  CAS  Google Scholar 

  5. N. Prabhakar, K. Arora, S.P. Singh, H. Singh, B.D. Malhotra, Anal. Biochem. 366, 71 (2007)

    Article  CAS  Google Scholar 

  6. Y. Miao, T.J. Cui, F.F. Leng, W.D. Wilson, Anal. Biochem. 374, 7 (2008)

    Article  CAS  Google Scholar 

  7. I.P. Chernov, K.A. Timchenko, S.B. Akopov, L.G. Nikolaev, E.D. Sverdlov, Anal. Biochem. 364, 60 (2007)

    Article  CAS  Google Scholar 

  8. S.Y. Niu, Q.Y. Li, R. Ren, S.S. Zhang, Biosens. Bioelectron. 24, 2943 (2009)

    Article  CAS  Google Scholar 

  9. S.C.B. Oliveira, O. Corduneanu, A.M. Oliveira-Brett, Bioelectrochemistry 72, 53 (2008)

    Article  CAS  Google Scholar 

  10. Y.Z. Zhang, K.Y. Zhang, H.Y. Ma, Anal. Biochem. 387, 13 (2009)

    Article  CAS  Google Scholar 

  11. M.S. Hejazi, M.H. Pournaghi-Azar, F. Ahour, Anal. Biochem. 399, 118 (2010)

    Article  CAS  Google Scholar 

  12. N. Nasirizadeh, H.R. Zare, M.H. Pournaghi-Azar, M.S. Hejazi, Biosens. Bioelectron. 26, 2638 (2011)

    Article  CAS  Google Scholar 

  13. F. Li, W. Chen, C.F. Tang, S.S. Zhang, Talanta 77, 1 (2008)

    Article  CAS  Google Scholar 

  14. M.H. Pournaghi-Azar, F. Ahour, M.S. Hejazi, Electroanalysis 21, 1822 (2009)

    Article  CAS  Google Scholar 

  15. K. Nakano, K. Nakamura, K. Iwamoto, N. Soh, T. Imato, J. Electroanal. Chem. 628, 113 (2009)

    Article  CAS  Google Scholar 

  16. P. Du, H.X. Li, W. Cao, Biosens. Bioelectron. 24, 3223 (2009)

    Article  CAS  Google Scholar 

  17. S.Y. Niu, M. Zhao, R. Ren, S.S. Zhang, J. Inorg. Biochem. 103, 43 (2009)

    Article  CAS  Google Scholar 

  18. T. Yang, W. Zhang, M. Du, K. Jiao, Talanta 75, 987 (2008)

    Article  CAS  Google Scholar 

  19. Y. Xu, X.Y. Ye, L. Yang, P.A. He, Y.Z. Fang, Electroanalysis 18, 1471 (2006)

    Article  CAS  Google Scholar 

  20. Y. Shacham-Diamand, E. Finkman, Y. Pinkas, N. Moriya, Appl. Phys. Lett. 59, 2953 (1991)

    Article  CAS  Google Scholar 

  21. W.J. Shi, S.Y. Ai, J.H. Li, L.S. Zhu, Chin. J. Anal. Chem. 36, 335 (2008)

    Article  CAS  Google Scholar 

  22. E. Laviron, J. Electroanal. Chem. 52, 355 (1974)

    Article  CAS  Google Scholar 

  23. H. Yu, Q. Sheng, J. Zheng, Electrochim. Acta 52, 4403 (2007)

    Article  CAS  Google Scholar 

  24. E. Laviron, J. Electroanal. Chem. 101, 19 (1979)

    Article  CAS  Google Scholar 

  25. G.X. Wang, Q.X. Pan, J.J. Zhang, H.S. Wang, Acta Chim. Sin. 64, 2259 (2006)

    CAS  Google Scholar 

  26. M. Liu, Q.X. Li, G.A. Rechnitz, Electroanalysis 12, 21 (2000)

    Article  CAS  Google Scholar 

  27. K.A. Fähnrich, M. Pravda, G.G. Guilbault, Biosens. Bioelectron. 18, 73 (2003)

    Article  Google Scholar 

  28. R. Doong, H.M. Shih, S.H. Lee, Sens. Actuat. B 111–112, 323 (2005)

    Article  Google Scholar 

Download references

Acknowledgments

The authors thank for the financial support of the National Basic Research Program of China (2009CB421601), Natural Science Foundation of China (21105030, 20827006), the Education Department for New Teacher of Guangdong Province, and the key Academic Program of 211 Project of South China Agricultural University (2009B010100001).

Author information

Authors and Affiliations

  1. Institute of Biomaterials, College of Sciences, South China Agricultural University, Guangzhou, 510642, People’s Republic of China

    Yingju Liu, Zhijun Ye, Chunping Wang, Mouhong Lin & Ying Luo

  2. School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou, 510275, People’s Republic of China

    Guang Shao

Authors
  1. Yingju Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Zhijun Ye
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Guang Shao
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Chunping Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Mouhong Lin
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Ying Luo
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yingju Liu.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Liu, Y., Ye, Z., Shao, G. et al. Construction of dsDNA biosensor based on dendritic SiO2 nanoparticle and investigation on its interaction with benzo[a]pyrene. J IRAN CHEM SOC 9, 129–136 (2012). https://doi.org/10.1007/s13738-011-0036-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13738-011-0036-5

Keywords

Search

Navigation