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Synthesis and Cytotoxicity Evaluation of Tropinone Derivatives

Natural Products and Bioprospecting volume 7, pages 215–223 (2017)Cite this article

Abstract

Sixteen tropinone derivatives were prepared, and their antitumor activities against five human cancer cells (HL-60, A-549, SMMC-7721, MCF-7 and SW480) were evaluated with MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxy methoxyphenyl)-2-(4-sulfopheny)-2H-tetrazolium] assay. Most of the derivatives exhibited better activities compared with tropinone at the concentration of 40 μM. Particularly, derivative 6 showed significant activities with IC50 values of 3.39, 13.59, 6.65, 13.09 and 12.38 μM respectively against HL-60, A-549, SMMC-7721, MCF-7 and SW480 cells, which suggested more potent activities than that of cis-dichlorodiamineplatinum (DDP).

Graphical Abstract

Introduction

Cancer, a diverse group of diseases characterized by those uncontrolled growth of tumor cells, is a leading cause of morbidity and mortality globally, which brings heavy economic burden to society and individuals [1, 2]. The conquest of cancer continued to pose great challenges to medical science since the pathogenesis is complicated and yet not well clarified [3–5]. With the understanding of cancer pathophysiology, some breakthrough therapies for the treatment of cancer were developed [6], but exploring novel types of antitumor drug is still needed.

Among the natural products studied in the 19th and early 20th centuries, tropane alkaloids attracted particular interest due to their potent and extensive biological activities [7], including regulating the secretion of monoamine neurotransmitter [8–11], glycine receptor [12], and acetylcholine receptor [13–15]. The structural scaffold of tropane is a bicyclic amine with a pyrrolidine and piperidine ring sharing a nitrogen atom and two carbon atoms. Tropinone as a natural tropane alkaloid mainly distributed in Solanaceae plants (Cyphomandra betacea) [16], and the total synthesis of tropinone in 1917 by Sir Robert Robinson represented a landmark achievement in organic synthesis [17]. α,β-Unsaturated ketone is a kind of important organic intermediate, which is widely used in the fields of medicine, chemistry, material science, biology and so on. Recent studies suggested that chalcones [18–20] and α,β unsaturated ketones (including coumarin [21], pyrimidine [22], thiosemicarbazide [23], imidazole [24], piperidine [25] analogues) had antitumor activities. Therefore, it is speculated that α,β unsaturated ketones with the structural scaffold of tropane may have antitumor activities. Thus we designed and synthesized a series of tropinone derivatives, and their preliminary biological evaluation was performed for their inhibitory activities in five human cancer cell lines (HL-60, A-549, SMMC-7721, MCF-7 and SW480) using MTS [3-(4, 5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfopheny)-2H-tetrazolium] method. The pattern of apoptosis in vitro against cancer depended upon cell line and dose of the compound [26, 27] and the dose was established according to the related literatures [28–30].

Results and Discussion

Chemistry

With an objective to obtain target compounds which have activities against five human cancer cells in vitro, tropinone was reacted with corresponding benzaldehyde or other aromatic aldehydes by Claisen-Schmidt condensation in the mixed solution of a catalytic amount of NaOH, and ethanol to generate compounds 1–16. The results suggested that introduction of 2,4-bis(phenylmethylene) groups improved activities compared with that of tropinone (Fig. 1).

Fig. 1
figure 1

Synthesis of the tropinone derivatives

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Biological Evaluation

Derivatives 2 and 8 were synthesized [31–34] and evaluated against Molt 4/C8, CEM cells and L1210 cells in vitro, which were effective indicators of derivatives having potential clinical utility. Derivatives 1–16 were tested for antitumor activities against five human cancer cells (HL-60, A-549, SMMC-7721, MCF-7 and SW480) by MTS method (Tables 1, 2).

Table 1 The inhibitory rates of tropinone derivatives against tumor cells in vitro
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Table 2 The IC50 (μM) values of derivatives 1, 6 and 9 against tumor cells in vitro
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Derivative 1 displayed potential inhibitory activity against HL-60 cell, which was very similar with that of derivative 9, and their inhibitory rates were up to 88.30 ± 3.33% and 81.56 ± 1.98% at the concentration of 40 μM, respectively. Derivatives 1 and 9 with unsubstituted and ortho- methoxyl substituted patterns at the phenyl ring, displayed inhibitory potency against HL-60 cell with the IC50 values of 13.62 and 18.97 μM, respectively. Compared with the tropinone, the inhibitory activities of derivative 6 (IC50 = 3.39 μM) showed a 3–9 fold enhancement and was better than that of the positive DDP. Derivatives 5, 10 and 14 possessed higher inhibitory activities than that of tropinone with the inhibitory rates of 55.69 ± 2.59, 59.19 ± 1.64 and 71.16 ± 2.43% at the concentration of 40 μM, respectively.

Derivatives 1 and 6 showed significantly inhibitory activities against A-549 cell with IC50 values of 16.78 and 13.59 μM, respectively. Derivatives 4, 9, 10 and 15 possessed moderate activities with inhibitory rates of 57.58 ± 2.99, 67.17 ± 2.80, 55.93 ± 0.96 and 54.41 ± 2.93% at the concentration of 40 μM, respectively.

Derivatives 1 and 9 displayed inhibitory potency against SMMC-7721 cell with IC50 values of 14.24 and 28.90 μM, respectively. Derivative 6 (IC50 = 6.65 μM) showed significant activity against SMMC-7721 cell, which exhibited more potential than that of DDP (IC50 = 13.86 μM).

Derivatives 1, 4, 6, 9 and 16 possessed activities against MCF-7 cell with inhibitory rates of 69.77 ± 1.70, 51.95 ± 0.22, 68.19 ± 1.38, 72.91 ± 0.12 and 60.74 ± 2.60% at the concentration of 40 μM, respectively. Particularly, Derivatives 1, 6 and 9 showed significant activities with IC50 values of 16.57, 13.09 and 16.31 μM, respectively.

Derivatives 4, 5, 9 and 16 demonstrated moderate inhibitory activity against SW480 cell with inhibitory rates of 67.73 ± 2.01, 54.20 ± 1.30, 87.84 ± 1.86% and 68.09 ± 1.12% at the concentration of 40 μM, respectively.

The Preliminary Structure–Activity Relationships (SARs)

SARs were discussed based on the bioassay results against five human cancer cells in vitro (Fig. 2). Derivative 1, with unsubstituted phenylmethylene group, exhibited better activities against five human cancer cells (IC50 = 13.62, 16.78, 14.24, 16.57 and 11.95, respectively). Derivatives 2, 3, 4 and 7 showed less potent activities, suggesting that the 2-halogenated or 2-methylation derivatives were unfavorable for maintaining activities. The chlorinated derivative 5 at meta-position of phenyl ring displayed slightly lower activities than that of bromated derivative 6 against five human cancer cells. Derivative 6 showed significant activities against HL-60, A-549, SMMC-7721, MCF-7 and SW480 cell (IC50 = 3.39, 13.59, 6.65, 13.09 and 12.38, respectively) and more potent activities than that of DDP. Derivative 9 with a methoxy group at the C-2 position of the phenyl ring indicated higher activities than that of dimethoxy substituted derivative 10 with 2,4-position at the phenyl ring. Inhibitory activities of derivatives 8, 11 and 12, with electron-donating group (–CH3) or an electron-acceptor groups (CF3, CN) at the para- phenyl ring, showed similar activity against five human cancer cells compared with that of tropinone. From the above results, it is suggested that 2,4-bis-phenylmethylene groups were favorable for inhibitory activities. When the 2,4-bis-phenylmethylene groups were changed to be 2,4-bis-4-heteroaryl-methylene groups, the inhibitory activities significantly decreased (derivatives 14-16 compared to 1-12), while 2,4-bis-naphthylmethylene groups (derivative 13) did not affect the inhibitory activities.

Fig. 2
figure 2

The inhibitory activities of the tropinone derivatives

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Conclusion

In summary, sixteen tropinone derivatives were synthesized and evaluated on HL-60, A-549, SMMC-7721, MCF-7 and SW480 cell lines in vitro. Among of them, derivatives (1, 4, 5, 6, 9, 10, 14, 15 and 16) exhibited higher cytotoxic activities. Particularly, derivatives 1, 6 and 9 had significantly improved activities against HL-60, A-549, SMMC-7721, MCF-7 and SW480 cells with IC50 values of 13.62, 16.78, 14.24, 16.57, 11.95 μΜ, 3.39, 13.59, 6.65, 13.09, 12.38 μΜ and 18.97, 29.23, 28.90, 21.14, 19.79 μΜ respectively. Structurally, the α, β-unsaturated ketone moiety was introduced into the tropinone scaffold, which should be responsible for enhanced activities. Among the optimized benzene ring derivatives, derivative 6 had higher cytotoxic activities than that of cis-DDP. The SARs of tropinone derivatives provided potential compounds for further investigation on antitumor regents screening.

Experimental Section

Materials and Instruments

The solvents were dried according to standard procedures. The organic solvents (analytical grade reagents) were purchased from Tianjin Chemical Reagent Co., Ltd (Tianjin, China). The tropinone and corresponding aldehydes were purchased from Alfa Aesar or J&K Scientific Ltd. 1HNMR and 13CNMR date were recorded in CDCl3 on a 400 MHz spectrometer (Bruker, Bremerhaven, Germany) with the tetramethylsilane (TMS) as the internal standard. Low-resolution mass spectra (MS) and high-resolution mass spectra (HRMS) were measured on Shimadzu liquid chromatography-mass spectrometry (LCMS)-ion trap (IT)-time of flight (TOF) (Shimadzu, Kyoto, Japan). All reaction were carried out under an air atmosphere and monitored by using thin-layer chromatography (TLC, 200-300 mesh, Qingdao Makall Group Co., Ltd; Qingdao, China). Melting points which are uncorrected were determined using a SGW® X-4B microscopic melting instrument from Shanghai Precision and Scientific Instrument Co., Ltd (China). The purity of the target compounds was determined by three solvent systems and by HPLC methods.

General Procedure for the Synthesis of the Tropinone Derivatives

Tropinone (2 mmol, 1 equiv.) and 10% NaOH (5 mL) were dissolved in ethanol (15 mL). To a solution of corresponding aldehydes (4.4 mmol, 2.2 equiv.) in ethanol (5 mL), the mixture was slowly dropped into the above solution at 0 °C for 20 min. The resulting solution was stirred at room temperature and monitored by TLC. Subsequently, the reaction mixture was neutralized with a solution of 5% HCl, extracted with EtOAc (3 × 30 mL) and washed with saturated Na2CO3 and saturated NaCl. The organic layer was dried over anhydrous Na2SO4 and concentrated to dryness under reduced pressure. Purification by column chromatography on silica gel Et2NH/MeOH/CHCl3 (2:6:92, v/v/v) to the target derivatives.

8-Methyl-2,4-bis(phenylmethylene)-8-aza-bicyclo[3.2.1]octan-3-one (1)

Yellow powder, mp 137.7–139.6 °C; yield 82%. 1H NMR (400 MHz, CDCl3) δ: 7.84 (s, 2H, H-9, 10), 7.45–7.26 (m, 10H, Ar), 4.40 (m, 2H, H-1, 5), 2.62–2.60 (m, 2H, H-6, 7), 2.30 (s, 3H, H-8), 2.04–2.03 (m, 2H, H-6, 7). 13C NMR (100 MHz, CDCl3) δ: 188.0 (s, C-3), 138.3 (s, C-2,4), 136.6 (d, C-9, 10), 135.1 (s, C-1′, 1″), 130.2 (d, C-4′, 4″), 128.8 (d, C-2′, 2″, 6′, 6″), 128.5 (d, C-3′, 3″, 5′, 5″), 60.8 (d, C-1, 5), 35.8 (s, C-8), 30.3 (t, C-6, 7). IR (KBr) ν: 3439, 2950, 1670, 1608, 1584, 1445, 1237, 1164, 946, 778, 692 cm-1. ESIMS: m/z 316 [M + H]+, HRESIMS: calc for C22H22NO [M + H]+ 316.1696, found 316.1669.

8-Methyl-2,4-bis(2′-fluoro-phenylmethylene)-8-aza-bicyclo[3.2.1]octan-3-one (2)

Yellow lamellar crystals (CHCl3: EtOH, 80:20, v/v), mp 155.9–156.0 °C; yield 86%, 1H NMR (400 MHz, CDCl3) δ: 7.84 (s, 2H, H-9,10), 7.38–7.10 (m, 8H, Ar–H), 4.22 (m, 2H, H-1, 5), 2.54–2.51 (m, 2H, H-6, 7), 2.30 (s, 3H, H-8), 2.01–1.97 (m, 2H, H-6, 7). 13C NMR (100 MHz, CDCl3) δ: 188.0 (s, C-3), 162.1 (d, C-2′), 159.6 (s, C-2″), 140.2 (s, C-2,4), 130.9 (d, C-9, 10), 130.8 (d, C-4′, 4″), 129.2 (d, C-6′, 6″), 123.9 (s, C-4′, 4″), 123.1 (s, C-1′, 1″), 115.9 (d, C-3′, 3″), 61.1 (d, C-1, 5), 35.4 (s, C-8), 29.9 (t, C-6, 7). IR (KBr) ν: 3439, 2943, 1672, 1612, 1589, 1435, 1218, 1056, 752, 685 cm−1. ESIMS: m/z 352 [M + H]+, HRESIMS: calc for C22H19NOF2 [M + H]+ 352.1507, found 352.1502.

8-Methyl-2,4-bis(2′-chloro-phenylmethylene)-8-aza-bicyclo[3.2.1]octan-3-one (3)

Yellow powder, mp 178.0–178.9 °C; yield 88%, 1H NMR (400 MHz, CDCl3) δ: 7.92 (s, 2H, H-9,10), 7.46–7.19 (m, 8H, Ar–H), 4.14 (m, 2H, H-1, 5), 2.49–2.47 (m, 2H, H-6, 7), 2.31 (s, 3H, H-8), 1.98–1.96 (m, 2H, H-6, 7). 13C NMR (100 MHz, CDCl3) δ: 188.2 (s, C-3), 139.8 (s, C-2, 4), 135.1 (s, C-2′, 2″), 133.7 (d, C-9, 10), 133.7 (s, C-1′, 1″), 130.5 (d, C-3′, 3″), 129.9 (d, C-4′, 4″), 129.8 (d, C-6′, 6″), 126.4 (d, C-5′, 5″), 60.6 (d, C-1, 5), 35.2 (s, C-8), 30.1 (t, C-6, 7). IR (KBr) ν: 3438, 2941, 1671, 1610, 1586, 1461, 1218, 1059, 753, 662 cm−1. ESIMS: m/z 384 [M + H]+, HRESIMS: calc for C22H19NOCl2 [M + H]+ 384.0916, found 384.0937.

8-Methyl-2,4-bis(2′-bromo-phenylmethylene)-8-aza-bicyclo[3.2.1]octan-3-one (4)

Yellow powder, mp 157.4–157.8 °C; yield 87%, 1H NMR (400 MHz, CDCl3) δ: 7.92 (s, 2H, H-9,10), 7.66–7.17 (m, 8H, Ar–H), 4.14 (m, 2H, H-1, 5), 2.48–2.47 (m, 2H, H-6, 7), 2.31 (s, 3H, H-8), 1.98–1.96 (m, 2H, H-6, 7). 13C NMR (100 MHz, CDCl3) δ: 188.2 (s, C-3), 139.7 (s, C-2, 4), 136.0 (d, C-9, 10), 135.5 (s, C-1′, 1″), 135.5 (s, C-2′, 2″), 133.7 (d, C-3′, 3″), 133.0 (d, C-5′, 5″), 129.9 (d, C-6′, 6″), 126.4 (d, C-4′, 4″), 60.6 (d, C-1, 5), 35.1 (s, C-8), 30.1 (t, C-6, 7). IR (KBr) ν: 3434, 2936, 1667, 1609, 1558, 1477, 1221, 1062, 942, 786, 688 cm−1. ESIMS: m/z 471 [M + H]+, HRESIMS: calc for C22H19NOBr2 [M + H]+ 471.9906, found 471.9928.

8-Methyl-2,4-bis(3′-chloro-phenylmethylene)-8-aza-bicyclo[3.2.1]octan-3-one (5)

Yellow powder, mp 136.9–137.8 °C; yield 86%, 1H NMR (400 MHz, CDCl3) δ: 7.71 (s, 2H, H-9,10), 7.34–7.21 (m, 8H, Ar–H), 4.30 (m, 2H, H-1, 5), 2.60–2.58 (m, 2H, H-6, 7), 2.28 (s, 3H, H-8), 2.00–1.96 (m, 2H, H-6, 7). 13C NMR (100 MHz, CDCl3) δ: 187.7 (s, C-3), 142.5 (d, C-9, 10), 139.6 (s, C-2, 4), 137.0 (s, C-3′, 3″), 130.3 (d, C-5′, 5″), 134.9 (d, C-5′, 5″), 129.4 (s, C-2′, 2″), 128.6 (d, C-4′, 4″), 127.1 (d, C-6′, 6″), 61.2 (d, C-1, 5), 36.2 (s, C-8), 30.5 (t, C-6, 7). IR (KBr) ν: 3437, 2936, 1667, 1609, 1563, 1477, 1221, 1062, 942, 786, 688 cm−1. ESIMS: m/z 384 [M + H]+, HRESIMS: calc for C22H19NOCl2 [M + H]+ 384.0916, found 384.0916.

8-Methyl-2,4-bis(3′-bromo-phenylmethylene)-8-aza-bicyclo[3.2.1]octan-3-one (6)

Yellow powder, mp 142.4–143.7 °C; yield 84%, 1H NMR (400 MHz, CDCl3) δ: 7.72 (s, 2H, H-9,10), 7.50–7.26 (m, 8H, Ar–H ‘), 4.32 (m, 2H, H-1, 5), 2.62–2.59 (m, 2H, H-6, 7), 2.30 (s, 3H, H-8), 2.02–1.97 (m, 2H, H-6, 7). 13C NMR (100 MHz, CDCl3) δ: 187.4 (s, C-3), 139.4 (d, C-2, 4), 137.0 (s, C-1′, 1″), 134.9 (d, C-9, 10), 132.9 (d, C-2′, 2″), 131.7 (d, C-4′, 4″), 130.0 (d, C-5′, 5″), 128.6 (d, C-6′, 6″), 122.6 (s, C-3′, 3″), 60.7 (d, C-1, 5), 35.9 (s, C-8), 30.1 (t, C-6, 7). IR (KBr) ν: 3435, 2929, 1668, 1608, 1556, 1470, 1220, 1058, 944, 787, 681 cm−1. ESIMS: m/z 471 [M + H]+, HRESIMS: calc for C22H19NOBr2 [M + H]+ 471.9906, found 471.9928.

8-Methyl-2,4-bis(2′-methyl-phenylmethylene)-8-aza-bicyclo[3.2.1]octan-3-one (7)

Yellow powder, mp 151.0–152.2 °C; yield 79%, 1H NMR (400 MHz, CDCl3) δ: 7.93 (s, 2H, H-9,10), 7.27–7.13 (m, 8H, Ar–H), 4.20 (m, 2H, H-1, 5), 2.51–2.48 (m, 2H, H-6, 7), 2.36 (s, 3H, H-8), 2.29 (s, 6H, Me), 2.00–1.98 (m, 2H, H-6, 7). 13C NMR (100 MHz, CDCl3) δ: 188.8 (s, C-3), 138.8 (s, C-2, 4), 138.0 (s, C-1′, 1″), 135.4 (d, C-9, 10), 134.3 (s, C-2′, 2″), 130.3 (d, C-4′, 4″), 129.2 (d, C-3′, 3″), 128.7 (d, C-6′, 6″), 125.6 (s, C-5′, 5″), 60.8 (d, C-1, 5), 35.6 (s, C-8), 30.3 (t, C-6, 7), 20.2 (s, C2′, 2″-Me). IR (KBr) ν: 3439, 2944, 1669, 1612, 1594, 1452, 1205, 1058, 925, 778 cm−1. ESIMS: m/z 344 [M + H]+, HRESIMS: calc for C24H25NO [M + H]+ 344.2009, found 344.1993.

8-Methyl-2,4-bis(4′-methyl-phenylmethylene)-8-aza-bicyclo[3.2.1]octan-3-one (8)

Yellow powder, mp 165.5–166.5 °C; yield 78%, 1H NMR (400 MHz, CDCl3) δ: 7.83 (s, 2H, H-9,10), 7.54 (d, J = 7.9 Hz, 4H, Ar–H), 7.23 (d, J = 7.9 Hz, 4H, Ar–H), 4.40 (m, 2H, H-1, 5), 2.63–2.60 (m, 2H, H-6, 7), 2.38 (s, 3H, H-8), 2.30 (s, 6H, Me), 2.04–1.99 (m, 2H, H-6, 7). 13C NMR (100 MHz, CDCl3) δ: 187.8 (s, C-3), 139.2 (s, C-2, 4), 137.5 (s, C-1′, 1″), 136.7 (d, C-9, 10), 132.2 (s, C-4′, 4″), 130.4 (d, C-3′, 3″, 5′, 5″), 129.3 (d, C-2′, 2″, 6′, 6″), 60.9 (d, C-1, 5), 35.7 (s, C-8), 30.3 (t, C-6, 7), 21.4 (s, C4′, 4″-Me). IR (KBr) ν: 3438, 2942, 1666, 1599, 1580, 1447, 1239, 1057, 935, 812 cm−1. ESIMS: m/z 344 [M + H]+, HRESIMS: calc for C24H25NO [M + H]+ 344.2009, found 344.1993.

8-Methyl-2,4-bis(2′-methoxyl-phenylmethylene)-8-aza-bicyclo[3.2.1]octan-3-one (9)

Yellow lamellar crystals (CHCl3: EtOH, 80:20, v/v), mp 155.5–155.7 °C; yield 70%, 1H NMR (400 MHz, CDCl3) δ: 8.02 (s, 2H, H-9,10), 7.35–6.90 (m, 8H, Ar–H), 4.27 (m, 2H, H-1, 5), 3.82 (s, 6H, OMe), 2.53–2.51 (m, 2H, H-6, 7), 2.30 (s, 3H, H-8), 2.02–1.99 (m, 2H, H-6, 7). 13C NMR (100 MHz, CDCl3) δ: 188.5 (s, C-3), 158.4 (s, C-2′, 2″), 138.2 (s, C-2, 4), 132.5 (d, C-9, 10), 130.5 (d, C-4′, 4″), 130.5 (d, C-6′, 6″), 124.3 (s, 1′, 1″), 120.0 (d, C-5′, 5″), 110.7 (d, C-3′, 3″), 61.0 (d, C-1, 5), 55.4 (s, C2′, 2″-OMe), 35.2 (s, C-8), 30.3 (t, C-6, 7). IR (KBr) ν: 3441, 2944, 1673, 1598, 1486, 1462, 1250, 1058, 757 cm−1. ESIMS: m/z 376 [M + H]+, HRESIMS: calc for C24H25NO3 [M + H]+ 376.1907, found 376.1906.

8-Methyl-2,4-bis(2′,4′-dimethoxy-phenylmethylene)-8-aza-bicyclo[3.2.1]octan-3-one (10)

Yellow oil, yield 79%,1H NMR (400 MHz, CDCl3) δ: 7.95 (s, 2H, H-9,10), 7.10–6.41 (m, 6H, Ar–H), 4.23 (m, 2H, H-1, 5), 3.78 (s, 12H, OMe), 2.53–2.49 (m, 2H, H-6, 7), 2.34 (s, 3H, H-8), 1.94–1.84 (m, 2H, H-6, 7). 13C NMR (100 MHz, CDCl3) δ: 188.0 (s, C-3), 161.7 (s, C-4′, 4″), 159.8 (s, C-2′, 2″), 136.3 (s, C-2, 4), 132.2 (d, C-9, 10), 131.1 (d, C-6′, 6″), 117.1 (s, C-1′, 1″), 104.1 (s, C-5′, 5″), 98.2 (d, C-3′, 3″), 60.9 (d, C-1, 5), 58.4 (s, C2′, 2″-OMe), 55.2 (s, C4′, 4″-OMe), 35.0 (s, C-8), 30.3 (t, C-6, 7). IR (KBr) ν: 3438.45, 2941.52, 1677.93, 1603.77, 1502.15, 1463.85, 1245.95, 1059.72, 810.43 cm−1. ESIMS: m/z 436 [M + H]+, HRESIMS: calc for C26H29NO5 [M + H]+ 436.2118, found 436.2128.

8-Methyl-2,4-bis(4′-trifluoromethyl-phenylmethylene)-8-aza-bicyclo[3.2.1]octan-3-one (11)

Yellow lamellar crystals (CHCl3: EtOH, 80:20, v/v), mp 172.8–173.2 °C; yield 88%, 1H NMR (400 MHz, CDCl3) δ: 7.81 (s, 2H, H-9,10), 7.68 (d, J = 7.8 Hz, 4H, Ar–H), 7.48 (d, J = 7.8 Hz, 4H, Ar–H), 4.33 (m, 2H, H-1, 5), 2.64–2.61 (m, 2H, H-6, 7), 2.30 (s, 3H, H-8), 2.05–2.02 (m, 2H, H-6, 7). 13C NMR (100 MHz, CDCl3) δ: 187.4 (s, C-3), 140.1 (s, C-2, 4), 138.4 (s, C-1′, 1″), 134.8 (d, C-9, 10), 130.3 (d, C-4′, 4″), 130.2 (d, C-3′, 3″, 5′, 5″), 125.5 (d, C-2′, 2″, 6′, 6″), 125.5 (s, C4′, 4″-CF3), 60.8 (d, C-1, 5), 35.9 (s, C-8), 30.1 (t, C-6, 7). IR (KBr) ν: 3440, 2935, 1672, 1609, 1587, 1411, 1244, 1065, 929, 842 cm−1. ESIMS: m/z 452 [M + H]+, HRESIMS: calc for C24H19NOF6 [M + H]+ 452.1444, found 452.1450.

8-Methyl-2,4-bis(4′-cyano-phenylmethylene)-8-aza-bicyclo[3.2.1]octan-3-one (12)

Yellow powder, mp 249.3–249.8 °C; yield 79%, 1H NMR (400 MHz, CDCl3) δ: 8.08 (s, 2H, H-9,10), 7.81–7.70 (m, 4H, Ar–H), 7.46–7.42 (m, 4H, Ar–H), 4.28 (m, 2H, H-1, 5), 2.63–2.60 (m, 2H, H-6, 7), 2.29 (s, 3H, H-8), 2.01–1.98 (m, 2H, H-6, 7). 13C NMR (100 MHz, CDCl3) δ: 187.1 (s, C-3), 140.7 (s, C-2, 4), 139.4 (s, C-1′, 1″), 134.2 (d, C-9, 10), 132.3 (d, C-3′, 3″, 5′, 5″), 130.5 (d, C-2′, 2″, 6′, 6″), 118.4 (s, C-4′, 4″), 112.3 (s, C4′, 4″-CN), 60.8 (d, C-1, 5), 36.0 (s, C-8), 29.9 (t, C-6, 7). IR (KBr) ν: 3431, 2942, 2227, 1670, 1605, 1585, 1501, 1241, 1060, 944, 835 cm−1. ESIMS: m/z 366 [M + H]+, HRESIMS: calc for C24H19N3O [M + H]+ 366.1601, found 366.1585.

8-Methyl-2,4-bis(2′-naphthylmethylene)-8-aza-bicyclo[3.2.1]octan-3-one (13)

Yellow powder, mp 257.6–258.1 °C; yield 78%, 1H NMR (400 MHz, CDCl3) δ: 8.02 (s, 2H, H-9,10), 7.90–7.86 (m, 8H, Ar–H), 7.54–7.52 (m, 6H, naphthalene-H), 4.54–4.53 (m, 2H, H-1, 5), 2.73–2.70 (m, 2H, H-6, 7), 2.48 (s, 3H, H-8), 2.19–2.17 (m, 2H, H-6, 7). 13C NMR (100 MHz, CDCl3) δ: 188.0 (s, C-3), 138.7 (s, C-2, 4), 136.7 (d, C-9, 10), 133.2 (s, C-1′, 1″), 133.1 (s, C-10′, 10″), 132.7 (s, C-9′, 9″), 130.3 (d, C-5′, 5″), 128.5 (d, C-4′, 4″), 128.2 (d, C-3′, 3″), 127.7 (d, C-7′, 7″), 127.4 (d, C-6′, 6″), 127.0 (d, C-8′, 8″), 126.6 (d, C-2′, 2″),61.1 (d, C-1, 5), 35.9 (s, C-8), 30.4 (t, C-6, 7). IR (KBr) ν: 3442, 2947, 1670, 1612, 1586, 1440, 1207, 1153, 1056, 940, 822, 747 cm−1. ESIMS: m/z 416 [M + H]+, HRESIMS: calc for C30H25NO [M + H]+ 416.2009, found 416.1996.

8-Methyl-2,4-bis(2′-thienylmethylene)-8-aza-bicyclo[3.2.1]octan-3-one (14)

Yellow powder, mp 163.6–164.5 °C; yield 74%, 1H NMR (400 MHz, CDCl3) δ: 7.92 (s, 2H, H-9,10), 7.52–7.11 (m, 6H, thiophene-H), 4.65 (m, 2H, H-1, 5), 2.61–2.58 (m, 2H, H-6, 7), 2.42 (s, 3H, H-8), 1.84–1.79 (m, 2H, H-6, 7). 13C NMR (100 MHz, CDCl3) δ: 186.6 (s, C-3), 138.3 (s, C-2, 4), 135.6 (s, C-2′, 2″), 133. 2 (d, C-9, 10), 130.1 (d, C-5′, 5″), 128.8 (d, C-3′, 3″), 127.9 (d, C-4′, 4″), 61.2 (d, C-1, 5), 36.2 (s, C-8), 29.7 (t, C-6, 7). IR (KBr) ν: 3437, 2944, 1655, 1589, 1450, 1417, 1239, 1166, 1040, 932, 853, 706 cm−1. ESIMS: m/z 328 [M + H]+, HRESIMS: calc for C18H17NOS2 [M + H]+ 328.0824, found 328.0808.

8-Methyl-2,4-bis(3′-thienylmethylene)-8-aza-bicyclo[3.2.1]octan-3-one (15)

Yellow powder, mp 169.9–170.8 °C; yield 77%, 1H NMR (400 MHz, CDCl3) δ: 7.78 (s, 2H, H-9,10), 7.74–7.22 (m, 6H, thiophene-H), 4.48 (m, 2H, H-1, 5), 2.63–2.61 (m, 2H, H-6, 7), 2.38 (s, 3H, H-8), 1.97–1.92 (m, 2H, H-6, 7). 13C NMR (100 MHz, CDCl3) δ: 187.4 (s, C-3), 136.8 (s, C-2, 4), 136.6 (s, C-3′, 3″), 130.1 (d, C-9, 10), 129.1 (d, C- 2′, 2″), 128.3 (d, C- 4′, 4″), 126.2 (d, C- 5′, 5″), 61.2 (d, C-1, 5), 36.0 (s, C-8), 30.0 (t, C-6, 7). IR (KBr) ν: 3444, 2934, 1673, 1608, 1582, 1380, 1240, 1204, 1152, 1058, 928, 789 cm−1. ESIMS: m/z 328 [M + H]+, HRESIMS: calc for C18H17NOS2 [M + H]+ 328.0824, found 328.0804.

8-Methyl-2,4-bis(2′quinolylmethylene)-8-aza-bicyclo[3.2.1]octan-3-one (16)

Yellow powder, mp 177.3–178.0 °C; yield 76%, 1H NMR (400 MHz, CDCl3) δ: 8.87 (d, J = 4.3 Hz, 2H, quinoline-H), 8.19 (s, 2H, H-9,10), 8.10–7.92 (m, 4H, quinoline-H), 7.69–7.49 (m, 4H, quinoline-H), 7.10 (d, J = 4.3 Hz, 2H, quinoline-H), 4.04 (m, 2H, H-1, 5), 2.38 (m, 2H, H-6, 7), 2.18 (s, 3H, H-8), 1.93–1.88 (m, 2H, H-6, 7). 13C NMR (100 MHz, CDCl3) δ: 187.3 (s, C-3), 149.6 (d, C-2′, 2″), 148.2 (s, C-4′, 4″), 142.4 (s, C-9′, 9″), 140.7 (s, C-2, 4), 1301.7 (d, C-9, 10), 130.0 (d, C-7′, 7″),129.8 (d, C-8′, 8″), 127.1 (d, C-6′, 6″), 126.7 (s, C-10′, 10″), 124.5 (d, C-5′, 5″), 120.7 (d, C-3′, 3″), 61.0 (d, C-1, 5), 35.820 (s, C-8), 29.9 (t, C-6, 7). IR (KBr) ν: 3441, 2943, 1683, 1612, 1580, 1563, 1503, 1417, 1388, 1204, 1166, 1061, 891, 845, 768 cm−1. ESIMS: m/z 418 [M + H]+, HRESIMS: calc for C28H23N3O [M + H]+ 418.1914, found 418.1932.

Biological Test Methods

The human tumor cell lines HL-60, SMMC-7721, A-549, MCF-7 and SW-480 were used, which were obtained from ATCC (Manassas, VA, USA). All cells were cultured in RPMI-1640 or DMEM medium (Hyclone, Logan, UT, USA), supplemented with 10% fetal bovine serum (Hyclone) and cultured with 95% O2/5% CO2 at 37 °C. Cell viability was assessed by conducting colorimetric measurements of the amount of insoluble formazan formed in living cells based on the reduction of 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfopheny)-2H-tetrazolium (MTS, Sigma, St. Louis, MO, USA) [35]. The cells were seeded in a Matrigel coated 96-well black plate with a plating volume of 100 μL/well at a density of 3000–15,000/well, and incubated in CO2 incubator to adhere for 12 h before the tested drugs were added. Tested derivatives and positive drug were dissolved in dimethyl sulfoxide (DMSO) and extracted a plating volume of 20 μL/well in the Matrigel coated 96-well clear plate. With cisplatin and paclitaxel as positive controls, each tumor cell line was exposed to the test derivatives at 40 μM concentrations in triplicate for 48 h. After the incubation, MTS (20 μL) was added to each well, and the incubation continued for 2–4 h at 37 °C. The optical density of lysate was measured at 492 nm in a 96-well by Multiskan FC (Thermo Scientific, US). The inhibition rates expressed as \({\bar{\text{X}}}\)  ± SD (n = 3) were obtained. The IC50 value of each derivative was calculated by Reed and Muench’s method [36].

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Acknowledgement

This work was supported by the Hundred Talents Program of the Chinese Academy of Sciences (CAS), the Youth Innovation Promotion Association, CAS and the Program of Yunling Scholarship.

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Affiliations

  1. State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, China

    Xiu-Juan Yin, Chang-An Geng, Xing-Long Chen, Chang-Li Sun, Tong-Hua Yang, Tian-Ze Li, Jun Zhou, Xue-Mei Zhang & Ji-Jun Chen

  2. Yunnan Key Laboratory of Natural Medicinal Chemistry, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, China

    Chang-An Geng, Tong-Hua Yang, Tian-Ze Li, Xue-Mei Zhang & Ji-Jun Chen

  3. University of Chinese Academy of Sciences, Beijing, 100049, China

    Xiu-Juan Yin & Xing-Long Chen

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Yin, XJ., Geng, CA., Chen, XL. et al. Synthesis and Cytotoxicity Evaluation of Tropinone Derivatives. Nat. Prod. Bioprospect. 7, 215–223 (2017). https://doi.org/10.1007/s13659-017-0124-z

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Keywords

  • Tropinone
  • Claisen-Schmidt reaction
  • MTS
  • Cytotoxicity