Abstract
A quantitative 1H NMR method (qHNMR) was used to measure the trigonelline content in the leaves of six species of the Annona genus. The methodology employed compared the intensities of the signals at δ 9.14 (H-2) and δ 0.00, the internal standard TSP-d4. This measuring method was able to establish the concentration of trigonelline in the range from 0.67 to 10.04 mg·g−1 depending on the investigated extract.
![](http://media.springernature.com/lw685/springer-static/image/art%3A10.1007%2Fs13659-013-0051-6/MediaObjects/13659_2013_51_Article_Fig1_HTML.gif)
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Blunden, G.; Cripps, A. L.; Gordon, S. M.; Mason, T. G.; Turner, C. H. Bot. Mar.1986, 29, 155–160.
Blunden, G.; Patel, A. V. Biochem. Syst. Ecol.2005, 33, 904–920.
Rochfort, S.; Parker, A. J.; Dunshea, F. R. Phytochemistry2008, 69, 299–322.
Corol, D. I.; Ravel, C.; Raksegi, M.; Bedo, Z.; Charmet, G.; Beale, M. H.; Shewry, P. R.; Ward, J. L. J. Agric. Food Chem.2012, 60, 5471–5481.
Sánchez-Hernández, L.; Castro-Puyana, M.; García-Ruiz, C.; Crego, A. L.; Marina, M. L. J. Agric. Food Chem.2010, 120, 921–928.
Tyihak, E.; Sarhan, A. R. T.; Cong, N. T.; Barna, B.; Kiraly, Z. Plant Soil1988, 109, 285–287.
Dini, I.; Tenore, G. C.; Trimarco, E.; Dini, A. Food Chem.2005, 98, 209–213.
Johns E. Berichte der Deutschen Chemischen Gesellschaft1985, 18, 2518–2523.
Mathur, M.; Kamal, R. Rev. Bras. Farmacogn.2012, 22, 994–1001.
Machado, A. R. T.; Campos, V. A. C.; Silva, W. R. J.; Campos, V. P.; Zeri, A. C. M.; Oliveira, D. F. Eur. J. Plant Pathol.2012, 134, 431–441.
Wishart, D. S. Trends Food Sci. Tech.2010, 19, 482–493.
Barbalho, M. S. Alvares Goulart, R.; Farinazzi-Machado, F. M. V.; Souza, M. S. S.; Cincotto Bueno, P. C. S.; Guiguer, E. L.; Araujo, A. C.; Groppo, M. Curr. Bioact. Compd.2012, 8, 277–286.
Pieri, V.; Belancic, A.; Morales, S.; Stuppner, H. J. Agric. Food Chem.2011, 59, 4378–4384.
Satheeshkumar, N.; Mukherjee, P. K.; Bhadra, S.; Saha, B. P. Phytomedicine2010, 17, 292–295.
Kim, H. K.; Choi, Y. A.; Verpoorte, R. Nat. Protoc.2010, 5, 36–49.
Author information
Authors and Affiliations
Corresponding author
Additional information
This article is published with open access at Springerlink.com
Rights and permissions
Open Access This article is distributed under the terms of the Creative Commons Attribution 2.0 International License (https://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
About this article
Cite this article
Machado, A.R.T., Lage, G.A., Medeiros, F.d.S. et al. Quantitative analysis of trigonelline in some Annona species by proton NMR spectroscopy. Nat. Prod. Bioprospect. 3, 158–160 (2013). https://doi.org/10.1007/s13659-013-0051-6
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s13659-013-0051-6