Abstract
This study explores the effects of halibut oil cream on different phases of wound healing and assess its potential as a supplement for promoting wound healing in rats. The study randomly assigned five groups of male wistar rats to receive different treatments: a vehicle-control group (water-soluble cream base), a positive-control group (Povidone-iodine ointment, PI-5%), and three halibut oil cream formulation (HBOF) treatment groups with strengths of 3, 9, and 27%. An excision wound model was used to induce injury and daily as well as terminal wound healing indices such as wound area contraction, relative wound area percentage, inflammatory and proliferative phases, oxidative stress, and cytokine levels were recorded. Compared to the vehicle control group, both the PI-5% and HBOF treatment groups significantly promoted wound healing. They demonstrated reduced wound size and oxidative stress in regenerated skin tissue. Histopathological examination revealed a marked increase in collagen production, and analysis of cytokine levels indicated enhanced efficacy. These effects can be attributed to the high hydroxyproline content of halibut oil. The findings of this study suggest that topically applying halibut oil cream may serve as a useful supplement for promoting wound healing in rats. The omega-3 fatty acids and other components present such as vitamin A and D in the cream formulation demonstrated beneficial effects on wound size reduction, oxidative stress reduction, collagen production, and cytokine efficacy.
Graphical abstract
Graphical Abstract depicting the experimental procedure for studying wound healing. Albino Wistar rats were utilized to create a 500 mm2 wound, with varying concentrations of HBOF applied topically from day (D) 1 to 15. The control group received treatment with a water-soluble cream base, while the standard group was treated with Povidone Iodine 5%. On day 16, the skin surrounding the wounds of all animals was carefully dissected, and biochemical, cytokine level, and histopathological evaluations were performed on the skin specimens. Throughout the study, measurements were taken for body weight, the inflammatory phase (D1-5), the proliferative phase (D5-15), and wound area contraction (D1-15).
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Abbreviations
- HBOF:
-
Halibut Oil Cream Formulation
- RT:
-
Retention Time
- D:
-
Day
- EPA:
-
Eicosapentaenoic acid
- DHA:
-
Docosahexaenoic acid
- SOD:
-
Superoxide Dismutase
- GSH:
-
Reduced Glutathione
- NBT:
-
Nitroblue Tetrazolium
- TMB:
-
3,3′,5,5′-Tetramethylbenzidine
- ROS:
-
Reactive Oxygen Species
- KOH:
-
Potassium hydroxide
- MPO:
-
Myeloperoxidase
- HCl:
-
Hydrochloric Acid
- ELISA:
-
Enzyme‐Linked Immunosorbent Assay
- TNF-α:
-
Tumor necrosis factor
- IL-6:
-
Interleukin-6
- IL-1β:
-
Interleukin-1β and TGF-β: Transforming growth factor‐beta
- PI-5%:
-
Povidone Iodine 5% w/w
- H&E Stain:
-
Hematoxylin and eosin Stain
- CCSEA:
-
Committee for the control and supervision of experiments on animals
- PRC:
-
Pretox Research Centre
- hrs:
-
Hours
- Secs:
-
Seconds
- SEM:
-
Standard Error Mean
- ANOVA:
-
Analysis of Variance
- HPLC:
-
High Performance Liquid Chromatography
- GC:
-
Gas Chromatography
- FID:
-
Flame Ionized Detector
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Acknowledgements
The authors are thankful to Shri JJT University, Jhunjhunu, Rajasthan, for providing the necessary facilities and support to carry out this research project. The authors are also grateful to Dr. Avijit Choudhury and Mr. Vishal Patel for their valuable support in carrying out the research work at Pretox Research Centre.
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STS: conceptualized, planned, and performed the project, developed the methodology, performed the formal analysis and data visualization, and wrote the original draft and edited the final manuscript. AK, SAA, and DK developed and supervised the methodology, performed the investigation and, edited and reviewed the manuscript. SKD conceptualized, visualized, data curation and supervised the project and edited and reviewed the manuscript.
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Animal experiment protocol was approved by the Institutional Ethics Animal Committee (Approval No: PRC/IAEC/2023/01/XIV/001), formed under the regulation of CCSEA, INDIA.
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S. T. Shukla has no conflict of interest. Anu Kaushik has no conflict of interest. Samiullah Allahbaksh Auti has no conflict of interest. Dinesh Kumar has no conflict of interest. Supriya Kumar Das has no conflict of interest.
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Shukla, S.T., Kaushik, A., Auti, S.A. et al. In-vivo assessment of wound healing activity of halibut oil cream in rat model of excision wound. ADV TRADIT MED (ADTM) (2024). https://doi.org/10.1007/s13596-024-00748-z
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DOI: https://doi.org/10.1007/s13596-024-00748-z