Abstract
Tetrapleura tetraptera Taub. (Fabaceae), commonly known as Aidan is ethnopharmacologically used for the management of health conditions such as diabetes mellitus, leprosy, epilepsy, and stroke in Nigeria. This study evaluated the anti-oxidoinflammatory properties of T. tetraptera methanol leaf extract (TTE) in lithium-pilocarpine-induced status epilepticus in Wistar rats. The extract was phytochemically screened and HPLC fingerprinting was performed. Animals were intraperitoneally administered with 127 mg/kg lithium chloride followed by 25 mg/kg pilocarpine 20 h later to induce status epilepticus. The animals were post treated with 50, 100 and 200 mg/kg TTE with 10 mg/kg valproic acid as the reference standard drug. Phytochemical screening of TTE confirmed the presence of tannins, phenols, alkaloids, terpenoids, flavonoids, saponins, glycosides and steroids. Quantitative phytochemical and antioxidant analyses of the extract indicated significant in vitro antioxidant and radical scavenging activity. HPLC analysis of the extract confirmed the presence of aridanin and polyphenols. TTE ameliorated redox imbalance by increasing markers for oxidative stress such as ferric reducing antioxidant power, glutathione level, catalase, glutathione S-transferase and superoxide dismutase activities. Moreover, TTE ameliorated pro-inflammatory events by reducing the level of the pro-inflammatory mediator nitric oxide, attenuating lipid peroxidation (which produces inflammatory lipid peroxidation-derived aldehydes), and decreasing the activities of xanthine oxidase and lactate dehydrogenase in the brain. These results indicated that the leaf of Tetrapleura tetraptera has therapeutic potential against status epilepticus by reversing oxidoinflammatory events. Tetrapleura tetraptera leaf extracts could be used to produce novel plant-based pharmaceuticals for treating status epilepticus and associated disorders.
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Abbreviations
- ABTS:
-
2,2′-Azino-bis (3-ethylbenzothiazoline-6-sulfonate) radical scavenging activity
- DPPH:
-
2,2-Diphenyl-1-picrylhydrazyl radical scavenging activity
- GSH:
-
Reduced glutathione
- H2O2 :
-
Hydrogen peroxide
- LDH:
-
Lactate dehydrogenase
- LPO:
-
Lipid peroxidation
- MDA:
-
Malondialdehyde
- NO:
-
Nitric oxide
- OH:
-
Hydroxyl radical
- RP:
-
Reducing power
- SE:
-
Status epilepticus
- SOD:
-
Superoxide dismutase
- TTE:
-
Tetrapleura tetraptera leaf extract
- XO:
-
Xanthine oxidase
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COO, OOO, ARA, JRA and ACA contributed to the design of the work. COO, OOO, ARA, JRA carried out the bench work. COO and OOO prepared the initial draft of the manuscript. ACA corrected the manuscript. MTO and ACA provided laboratory space and supervised the work. All authors agreed to the final form of the manuscript.
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The experiments were approved by the animal research ethics committee of FUTA (Ethical Approval No.: FUTA/ETH/21/04).
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Comfort O. Oladejo has no conflict of interest. Omowumi O. Ogundele has no conflict of interest. Abimbola R. Adeoti has no conflict of interest. Jesutola R. Atilola has no conflict of interest. M. Tolulope Olaleye has no conflict of interest. Afolabi C. Akinmoladun has no conflict of interest.
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Oladejo, C.O., Ogundele, O.O., Adeoti, A.R. et al. Tetrapleura tetraptera curtails oxidative and proinflammatory biochemical events in lithium-pilocarpine model of status epilepticus. ADV TRADIT MED (ADTM) 23, 1209–1220 (2023). https://doi.org/10.1007/s13596-022-00675-x
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DOI: https://doi.org/10.1007/s13596-022-00675-x