Abstract
Hill tract plant Tetrastigma bracteolatum (Wall.) (Family-Vitaceae) is traditionally used for headache, indigestion and stomach disorder by the tribal people in Bangladesh. In this study, the ethanolic leaves extract of TB was investigated for screening its phytochemical constituents and assessing the antioxidant, analgesic, antidiarrheal and antihyperglycemic potential. In DPPH free radical scavenging assay, the extract showed good radical scavenging potential with SC50 (concentration for 50% scavenging) value of 56 µg/ml. Analgesic activity test was performed by acetic acid-induced writhing method, where the extract inhibited writhing by 28.8% and 51.5% at the doses of 250 and 500 mg/kg bw, respectively. The extract inhibited defecation by 36.2% and 60.3% at the doses of 250 and 500 mg/kg bw in castor oil-induced diarrheal mice. In the evaluation of antihyperglycemic activity by oral glucose tolerance test (OGTT), the extract reduced blood glucose levels in a dose-dependent manner after 90 min, compared to the control group. The extract was also assessed for its in vitro α-glucosidase inhibition capacity but no demonstrable effect was observed. Fourteen chemical compounds were identified through GC–MS analysis of the extract with the highest amount of 1,2,3,4,4a,5,8,9,12,12a-decahydro-1,4-methanobenzocyclodecene (5.46%). Few compounds displayed better binding affinity with the active site of NADPH oxidase, nitric oxide synthase, M3 muscarinic acetylcholine receptor and sulfonylurea receptor than standard drugs in molecular docking studies. Therefore, the extract of TB may be used as a potential source of drug leads for various ailments.
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Abbreviations
- TB:
-
Tetrastigma bracteolatum
- DPPH:
-
1,1-Diphenyl-2-picrylhydrazyl
- mg/kg bw:
-
Milligram per kilogram body weight
- min:
-
Minute
- OGTT:
-
Oral glucose tolerance test
- NOX:
-
NADPH oxidase
- NOS:
-
Nitric oxide synthase
- SUR1:
-
Sulfonylurea receptor
- ROS:
-
Reactive oxygen species
- RNS:
-
Reactive nitrogen species
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Acknowledgements
The authors are grateful to Pharmacy Discipline, Khulna University for providing the laboratory facilities, instrumental support as well as chemicals and reagents required for conducting this research. Again, the authors would like to express their gratitude to the experts of Bangladesh National Herbarium for the identification of the plant species. The authors showed sincere gratitude to the Department of Pharmacy, Jahangirnagar University, for supplying the experimental mice.
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MNI: Conceptualization, methodology, writing- original draft preparation, conduction of all laboratory tests, literature search, statistical analysis, in silico analysis. HSD: Assistance in conduction of tests, in silico analysis. MMM: Assistance in conduction of tests, resources, formal analysis. RPB: Assistance in conduction of tests, validation. NNB: Writing and editing original paper. BB: GC-MS analysis and result interpretation. SKS: Project design, idea generation, resources, data curation, paper writing and reviewing, overall supervision.
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All animal experiments were conducted in accordance with the guidelines of the Khulna University Animal Ethics Committee under Khulna University Research Cell.
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Md. Naharul Islam has no conflict of interest. Hiron Saraj Devnath has no conflict of interest. Maisha Maliha Medha has no conflict of interest. Ritu Porna Biswas has no conflict of interest. Nripendra Nath Biswas has no conflict of interest. Biswajit Biswas has no conflict of interest. Samir Kumar Sadhu has no conflict of interest.
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Islam, M.N., Devnath, H.S., Medha, M.M. et al. In silico profiling of analgesic, antidiarrheal and antihyperglycemic properties of Tetrastigma bracteolatum (Wall.) leaves extract supported by in vivo studies. ADV TRADIT MED (ADTM) 23, 789–801 (2023). https://doi.org/10.1007/s13596-022-00641-7
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DOI: https://doi.org/10.1007/s13596-022-00641-7