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Properties of Solanum melongena green calyx against toxic effects of diabetes‐induced testopathy: a stereological and biochemical study

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Abstract

Solanum melongena green calyx (SMGC) with antioxidant features seems to have many therapeutic features. Diabetes mellitus (DM) is a blood sugar disorder that increases oxidative stress levels in various types of cells leading to testopathy. In this study we aimed to investigate the protective functions of hydro-alcoholic extract of SMGC on DM‐induced testicular damages. Mature male rats were randomly divided into five groups (n = 8); 1: control, 2: diabetic, 3: diabetic + 100 mg/kg of SMGC, 4: diabetic + 300 mg/kg of SMGC, and 5: diabetic + 500 mg/kg of SMGC. Intraperitoneal injection of streptozotocin (STZ, a single dose of 60 mg/kg) was used for DM induction. Testicular alterations were evaluated quantitatively based on stereological techniques and followed by antioxidant enzymes determination (catalase, superoxide dismutase, and glutathione peroxidase), and serum testosterone level. Malondialdehyde and Bcl‐2 protein expression were also assessed biochemically. DM caused significant harmful changes in testicular structure, inhibition of antioxidant enzymes activity, decreased testosterone levels, and increased lipid peroxidation (LP). The expression of Bcl‐2 protein was down-regulated in DM testis along with boosted apoptosis rate. Eight weeks of SMGC treatment (particularly in high doses) recovered structural variations significantly, restored the power of the antioxidant defense system, and modified the testosterone levels. These findings revealed that the SMGC is considered as an effective antioxidant agent to decrease the adverse effects of DM on male reproductive system.

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Acknowledgements

We gratefully acknowledge the Research Council of Kermanshah University of Medical Sciences. Funding was provided by Kolej Universiti Sains Perubatan Cyberjaya (Grant No. 95202).

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Authors and Affiliations

  1. Department of Anatomical Sciences, School of Medicine, Immunology of Infectious Diseases Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran

    Ahmad Shabanizadeh

  2. Department of Anatomical Sciences, Medical School, Kermanshah University of Medical Sciences, Kermanshah, Iran

    Shiva Roshankhah, Amir Abdolmaleki & Mohammad Reza Salahshoor

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  1. Ahmad Shabanizadeh
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  2. Shiva Roshankhah
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  3. Amir Abdolmaleki
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  4. Mohammad Reza Salahshoor
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Corresponding author

Correspondence to Mohammad Reza Salahshoor.

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Ethical statement

This study was approved by the Ethical Committee of Kermanshah University of Medical Sciences (IR.KUMS.REC.1395.202), and written informed consent was obtained from every patient who participated in the research.

Conflict of interest

Ahmad Shabanizadeh has no conflict of interest. Shiva Roshankhah has no conflict of interest. Amir Abdolmaleki has no conflict of interest. Mohammad Reza Salahshoor has no conflict of interest.

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Shabanizadeh, A., Roshankhah, S., Abdolmaleki, A. et al. Properties of Solanum melongena green calyx against toxic effects of diabetes‐induced testopathy: a stereological and biochemical study. ADV TRADIT MED (ADTM) 22, 569–579 (2022). https://doi.org/10.1007/s13596-021-00568-5

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  • DOI: https://doi.org/10.1007/s13596-021-00568-5

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