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Histomorphometric and collagen fibers organization in annular punctured induced intervertebral disc degeneration in rabbit model: restorative and antioxidant role of Brassica oleracea

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Abstract

The prevalence of low back pain associated with disc degeneration reduced quality of life with socio-economic impact. This study is aimed at investigating the histomorphometric and antioxidant response of Brassica oleracea extract in annular puncture-induced intervertebral disc degeneration using a rabbit model. Fifteen (15) New Zealand white rabbits were used in this study and were divided into five groups (n = 3). Group A (non-punctured) received distilled water as placebo; group B underwent annular puncture for 4 weeks; group C underwent annular puncture for 8 weeks; group D underwent annular puncture for 2 weeks before receiving 500 mg/kg bw of Brassica oleracea extract for 2 weeks; group E underwent annular puncture for 4 weeks before receiving 500 mg/kg bw of Brassica oleracea extract for 4 weeks. The rabbits were sacrificed at 4 and 8 weeks after treatment, the discs were extracted and the histological, stereological and biochemical analysis were performed. Histological results showed lack of NP cells, disorganization in the arrangement of collagen fibres in the punctured control group (groups B and C), which was restored by Brassica oleracea extract administration after 2 and 4 weeks of treatment in the punctured-treated groups (D and E). Oxidative stress markers (CAT, SOD and GSH) levels were significantly reduced with corresponding increase in MDA among punctured-control groups but restored after 2 and 4 weeks of treatment in the punctured-treated groups. Administration of Brassica oleracea extract revealed a preventive and restorative potential in the cytoarchitecture and antioxidant level of the disc induced by annular puncture.

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Abbreviations

AF:

Annulus Fibrosus

CLC:

Chondrocyte like cells

CAT:

Catalase

SOD:

Sodium dismutase

AGG:

Aggrecanase

COL 1:

Collagen type 1

COL 2:

Collagen type 2

NP:

Nucleus pulposus

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Acknowledgements

The authors are grateful to Omomoh, Bernand in herbarium section of Centre for Research and Development (CERAD) of the Federal University of Technology, Akure for the identification and authentication of Brassica oleracea; Dr. IjomoneNeuro Laboratory, Department of Human Anatomy, Federal University of Technology Akure Nigeria for the photomicrograph capturing of slides and Mr. Ige of Histology Laboratory, Department of Anatomy and Cell Biology, Obafemi Awolowo University, Ife, Nigeria for preparation of histology slide.

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This article was not funded by any institution.

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Authors and Affiliations

  1. Human Anatomy Department, Federal University of Technology Akure, Ondo State, Nigeria

    B. Ogunlade, S. A. Adelakun, B. K. Akinola & O. A. Adedotun

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  1. B. Ogunlade
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  2. S. A. Adelakun
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  3. B. K. Akinola
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  4. O. A. Adedotun
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Correspondence to B. Ogunlade.

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Ethical statement

The experimental procedures were conducted in accordance with the NIH guidelines for the care and use of laboratory animals in line with guidelines of the Department of Human Anatomy, Federal University of Technology, Akure and the Health Research and Ethical Committee of the College of Medicine, University of Lagos: CM/HREC/07/2019/129.

Conflict of interest

B. Ogunlade has no conflict of interest. S. A. Adelakun has no conflict of interest. B. K. Akinola has no conflict of interest. O. A. Adedotun has no conflict of interest.

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The article involve animal study which was approved by the ethical committee of the institution.

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Ogunlade, B., Adelakun, S.A., Akinola, B.K. et al. Histomorphometric and collagen fibers organization in annular punctured induced intervertebral disc degeneration in rabbit model: restorative and antioxidant role of Brassica oleracea. ADV TRADIT MED (ADTM) 21, 341–350 (2021). https://doi.org/10.1007/s13596-021-00560-z

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