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Influence of harvest season and different polarity solvents on biological activities, phenolic compounds and lipid-soluble pigment contents of Spirogyra sp. from Algeria

Abstract

Effects of extraction solvent and harvest period on phytochemical analysis and antioxidant and antimicrobial activities of extracts obtained from the freshwater macroalga, Spirogyra sp. were evaluated. The experiment lasted five months, where five crude extracts were obtained by maceration using different solvents: hexane, chloroform, dichloromethane, acetone and methanol. Higher values of yields were recorded in methanolic extracts on February (7.304%) and March (4.812%). All extracts were rich in proanthocyanidins compared to total phenolics and flavonoids. The total amounts of carotenoids were very low (0.060 ± 0001 to 0.171 ± 0.005 mg/g) compared to chlorophylls which were higher in February (6.365 ± 0.075 mg/g) and in March (5.501 ± 0.118 mg/g). The antioxidant activities indicated that the chloroformic and dichloromethanic extracts of February and March exhibited a good activity. The correlation using the Pearson test of phenolic compounds and antioxidant activity tests have confirmed the strong contribution of flavonoids and proanthocyanidins. The greatest antimicrobial activity was found in hexanic extracts at 0.1 to 1 mg/disk. The present findings suggest that Spirogyra sp. can be exploited not only for human health, but also for screening new economic natural antioxidants that could be used in foods, cosmetics and pharmaceutical industries.

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Acknowledgements

The authors sincerely thank Pr. Choayb HADJADJ, Department of English, Faculty of Letters and Languages, University of Tlemcen, Algeria, for his sincere help in spelling and linguistic revision and correction.

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Correspondence to Nabila Belyagoubi-Benhammou.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Larbi Belyagoubi has no conflict of interest. Nabila Belyagoubi‑Benhammou has no conflict of interest. Fawzia Atik-Bekkara has no conflict of interest. Djamel Eddine Abdelouahid has no conflict of interest.

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Belyagoubi, L., Belyagoubi-Benhammou, N., Atik-Bekkara, F. et al. Influence of harvest season and different polarity solvents on biological activities, phenolic compounds and lipid-soluble pigment contents of Spirogyra sp. from Algeria. ADV TRADIT MED (ADTM) 22, 359–369 (2022). https://doi.org/10.1007/s13596-021-00551-0

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Keywords

  • Spirogyra sp.
  • Freshwater
  • Phytochemical study
  • Antioxidant activity
  • Antimicrobial activity