Abstract
PCOS is considered as common endocrine diseases among the females in their regenerative age. The purpose of the study was to analyze phytochemicals by GC–MS followed by the pharmacological evaluation of Sukumaram Kasayam on PCOS induced rats. GC–MS analysis of Sukumaram Kasayam was accomplish to identify phytoconstituents. Twenty-four female rats were used (n = 6/group) in the study consisting of healthy control (Group-I). The experimental groups were divided into 3 subgroups, including rats with PCOS which received no treatment (Group-II), PCOS group (Group-III and Group-IV) was administered daily dose of Clomifene citrate 200 mg/kg and Sukumaram Kasayam 0.5 ml/kg for 3 weeks, respectively. In all experimental groups, oral dose of 1 mg/kg letrozole for 21 days to induce PCOS. After treatment period, rats were anesthetized, blood sex hormone and lipid profile was carried. The GC–MS analysis identified Butane, 1, 1-diethoxy-3-, (+)-2-Bornanone abundant compound in formulation. The PCOS induced rats were treated with Clomiphene citrate, Sukumaram Kasayam, the testosterone decreased significantly (P < 0.01), progesterone increased significantly (P < 0.01), estradiol increased significantly (P < 0.01), the serum LH decreased (P < 0.05), the FSH levels were decreased significantly (P < 0.01). In case of the lipid profile the result showed that the triglyceride, cholesterol, LDL decreased significantly (P < 0.01), whereas the HDL increased significantly (P < 0.01) in comparison to PCOS control rats. Results concluded that Sukumaram Kasayam has beneficial effect on levels of the testosterone, progesterone, estradiol, LH, and FSH in alleviating the complications of PCOS.
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Acknowledgements
The authors are grateful to the management members and Principal, Institute of Pharmacy & Technology, Salipur for providing all facilities and encouragement throughout the work. The authors would like to thanks the CIL/SAIF Panjab university, Chandigarh for carrying out the GC–MS analysis of Sukumaram Kasayam.
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This article contain studies with animals. The experimental protocol related to animal was duly approved by the Institutional Animal Ethics Committee with approval number 43/IAEC/IPT/19.
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Sujit Dash has no conflict of interest. Sunil Kumar Kanungo has no conflict of interest. Bishwanath Mishra has no conflict of interest. Amaresh Chandra Sahoo has no conflict of interest. Aswini Kumar Senapati has no conflict of interest. Sangyarani Pattnaik has no conflict of interest.
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Dash, S., Kanungo, S.K., Mishra, B. et al. Evaluation of classical ayurvedic medicine “Sukumaram Kasayam” in a rat model of letrozole induced polycystic ovaries. ADV TRADIT MED (ADTM) 21, 675–683 (2021). https://doi.org/10.1007/s13596-020-00474-2
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DOI: https://doi.org/10.1007/s13596-020-00474-2