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Comparative pharmacologic profiles of leaves and hypocotyls of a mangrove plant: Bruguiera gymnorrhiza

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Abstract

Ethanolic extracts of leaves and hypocotyls of a mangrove plant, Bruguiera gymnorrhiza (Family Rhizophoraceae), were investigated to identify the presence of different phytochemicals and to assess the antihyperglycemic, antidiarrheal, analgesic and antioxidative activity in the current study. Oral glucose tolerance test (OGTT) was performed to investigate the in vivo antihyperglycemic activity and leaves extract (LE) was found to possess significant antihyperglycemic property compared to hypocotyl extract (HE). Therefore, the leaves extract was considered for α-Glucosidase enzyme inhibitory activity test and substantial α-glucosidase enzyme inhibitory property was observed with an IC50 (concentration required to inhibit 50% enzyme) value of 2.670. Antioxidative activity of plant extracts was tested in vitro by evaluating their ability to scavenge DPPH, hydrogen peroxide and hydroxyl radical as well as superoxide anions. In all cases, LE showed greater activity than HE. In addition, LE demonstrated higher amount of total phenolics, flavonoids and tannins indicating higher antioxidant effect compared to HE. For the evaluation of antidiarrheal effect in castor oil induced diarrheal mice, both extracts showed significant inhibition of defecation at doses 250 and 500 mg/kg body weight. Finally, for acetic acid-induced writhing method to evaluate the analgesic activity, significant percentage inhibition of writhing (P < 0.05 vs. control) was observed for both extracts at lower and higher doses. Altogether, these studies justified the plant’s usage in folklore medication.

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Acknowledgements

Ministry of Education, Bangladesh funded this project as part of an Advanced Research Aid Program in education. The authors are also thankful to the Khulna University Research Cell (KURC) for partial funding. The authors would also like to express their gratitude towards the authority of Pharmacy Discipline, Khulna University for providing the laboratory facilities, experimental instruments and whole hearted support to carry out the research project.

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Authors and Affiliations

  1. Pharmacy Discipline, Life Science School, Khulna University, Khulna, 9208, Bangladesh

    Mimi Golder, Samir Kumar Sadhu, Biswajit Biswas & Tannami Islam

  2. Department of Pharmacy, Faculty of Biological Science and Technology, Jashore University of Science and Technology, Jashore, 7408, Bangladesh

    Biswajit Biswas

Authors
  1. Mimi Golder
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  2. Samir Kumar Sadhu
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  3. Biswajit Biswas
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Correspondence to Samir Kumar Sadhu.

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Ethical statement

All experimental procedures performed in current study involving animals were in accordance with the ethical standards of Animal Ethics Committee (AEC), Khulna University, Khulna-9208, Bangladesh [Ref: KUAEC-2019/07/04].

Conflict of interest

Mimi Golder has no conflict of interest. Samir Kumar Sadhu has no conflict of interest. Biswajit Biswas has no conflict of interest. Tannami Islam has no conflict of interest.

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Golder, M., Sadhu, S.K., Biswas, B. et al. Comparative pharmacologic profiles of leaves and hypocotyls of a mangrove plant: Bruguiera gymnorrhiza. ADV TRADIT MED (ADTM) 20, 395–403 (2020). https://doi.org/10.1007/s13596-019-00423-8

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  • DOI: https://doi.org/10.1007/s13596-019-00423-8

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