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Gallic acid protects against neurochemical alterations in transgenic Drosophila model of Alzheimer’s disease

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Abstract

This study evaluated the effect of gallic acid on some selected biomarkers in Drosophila melanogaster model of Alzheimer’s disease (AD). Transgenic D. melanogaster expressing human amyloid precursor protein and β-secretase (BACE 1) genes were used as AD flies while wild type (Oregon strain) flies served as the normal control flies. Both fly strains were exposed to gallic acid in their diet. Thereafter, the flies were sacrificed and homogenized. The homogenates were assayed for reactive oxygen species (ROS), malondialdehyde (MDA) and total thiol contents, as well as the activity of catalase. Also the activity of cholinesterases (ChEs) and β-secretase (BACE-1) were quantified. Results showed that the AD flies had significantly higher ChEs and BACE-1 activity, ROS and MDA contents, as well as lower total thiol level and catalase activity compared to the normal control flies. However, these biochemical impairments in AD control flies were significantly ameliorated in AD flies treated with gallic acid. Therefore, this study has shown that gallic acid could ameliorate elevated ChEs and BACE-1 activities, as well as oxidative stress induced by β-amyloid generation in D. melanogaster model of AD. This therefore, suggests gallic acid as a promising nutraceutical for the management of AD.

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Abbreviations

AChE:

Acetylcholinesterase

BChE:

Butyryllcholinesterase

AD:

Alzheimer’s disease

APP:

Amyloid precursor protein

BACE-1:

β-Secretase

DCF:

2′,7′-Dichloroflourescein

DEPPD:

N,N-Diethyl-para-phenylenediamine

DL-BAPNA:

N-α-Benzoyl-d,l-arginine p-nitroanilide

DMSO:

Dimethyl sulfoxide

RONS:

Reactive oxygen/nitrogen species

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Acknowledgements

The authors wish to appreciate Dr. Daniel R. Marenda of the Department of Biology, University of Drexel, Philadelphia, USA for the generous gift of the transgenic Alzheimer’s disease flies used for this study

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Authors and Affiliations

  1. Department of Biomedical Technology, Federal University of Technology, P.M.B. 704, Akure, Nigeria

    Opeyemi B. Ogunsuyi

  2. Department of Biochemistry, Federal University of Technology, P.M.B. 704, Akure, Nigeria

    Opeyemi B. Ogunsuyi, Ganiyu Oboh, Odunayo O. Oluokun & Adedayo O. Ademiluyi

  3. Department of Biochemistry and Molecular Biology, Obafemi Awolowo University, Ile-Ife, Nigeria

    Omodesola O. Ogunruku

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  1. Opeyemi B. Ogunsuyi
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  2. Ganiyu Oboh
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Correspondence to Ganiyu Oboh.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Opeyemi B. Ogunsuyi has no conflict of interest. Ganiyu Oboh has no conflict of interest. Odunayo O. Oluokun has no conflict of interest. Adedayo O. Ademiluyi has no conflict of interest. Omodesola O. Ogunruku has no conflict of interest.

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Ogunsuyi, O.B., Oboh, G., Oluokun, O.O. et al. Gallic acid protects against neurochemical alterations in transgenic Drosophila model of Alzheimer’s disease. ADV TRADIT MED (ADTM) 20, 89–98 (2020). https://doi.org/10.1007/s13596-019-00393-x

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