Skip to main content

Advertisement

SpringerLink
Log in

Phytochemical screening and evaluation of pharmacological activities of aqueous extract of Typhonium trilobatum (L.) Schott

  • Research Article
  • Published:
Oriental Pharmacy and Experimental Medicine Aims and scope Submit manuscript

Abstract

This study aimed at phytochemical analysis and evaluation of toxic and protective effects of the crude aqueous extract of Typhonium trilobatum. Toxicity analysis was done in Allium cepa, while anti-inflammatory, and membrane stabilizing and clotlysis capacity were tested by using egg albumin and human erythrocytes, respectively. The results suggest that T. trilobatum aqueous extract contains alkaloids, glycosides, tannins, saponins, flavonoids and reducing sugars. The crude extract exhibited toxicity in A. cepa in a time and concentration dependent manner. The highest root growth inhibition was observed at exposure time 72 h in 20% (v/v) concentration extract of the herb. The extract at low concentration (2.5 and 5%) reduced the root growth inhibition capacity at 48 h in comparison to 24 and 72 h, possibly by adaptation of DNA damage profile in the eukaryotic test system (A. cepa), which was further confirmed in anti-inflammatory and membrane stabilizing, and anti-atherothrombosis test. In the latter cases, the extract at 0.02–0.08% (v/v) significantly (p < 0.05, p < 0.01, p < 0.001) inhibited protein denaturation, membrane lysis, while enhanced clotlysis capacity in a concentration-dependent manner. In conclusion, the extract exerted toxic effects at high concentrations and protective effects at low concentrations. T. trilobatum may be one of the potential sources of phytotherapeutic agents, especially for the treatment of inflammatory and cardiovascular diseases and disorders.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Adams DS, Griffin LA, Nachajko WR, Reddy VB, Wei CM (1991) A synthetic DNA encoding a modified human urokinase resistant to inhibition by serum plasminogen activator inhibitor. J Biol Chem 266(13):8476–8482

    CAS  PubMed  Google Scholar 

  • Adeyemo OA, Farinmade AE (2013) Genotoxic and cytotoxic effects of food flavor enhancer, monosodium glutamate (MSG) using Allium cepa assay. Afr J Biotechnol 12:1459–1466

    CAS  Google Scholar 

  • Ali K, Ashraf A, Biswas NN (2012) Analgesic, anti-inflammatory and anti-diarrheal activities of ethanolic leaf extract of Typhonium trilobatum L. Asian Pac J Tropic Biomed 2:722–726

    Article  Google Scholar 

  • Arawwawala M, Thabrew I, Arambewela L, Handunnetti S (2010) Anti-inflammatory activity of Trichosanthes cucumerina Linn. in rats. J Ethnopharmacol 131(3):538–543

    Article  PubMed  Google Scholar 

  • Árvay J, Šnirc M, Hauptvogl M, Bilčíková J, Bobková A, Demková L, Hudáček M, Hrstková M, Lošák T, Král M, Kováčik A, Štefániková J (2018) Concentration of micro- and macro-elements in green and roasted coffee: influence of roasting degree and risk assessment for the consumers. Biol Trace Elem Res. https://doi.org/10.1007/s12011-018-1519-3

    Article  PubMed  Google Scholar 

  • Bag A, Bhattacharyya SK, Pal NK, Chattopadhyay R (2013) Anti-inflammatory, anti-lipid peroxidative, antioxidant and membrane stabilizing activities of hydroalcoholic extract of Terminalia chebula fruits. Pharm Biol 51(12):1515–1520

    Article  CAS  PubMed  Google Scholar 

  • Balunas MJ, Kinghorn AD (2005) Drug discovery from medicinal plants. Life Sci 78(5):431–432

    Article  CAS  PubMed  Google Scholar 

  • Begum F (2002) The present status of medicinal plants in Bangladesh. Iran J Pharm Res 3:34–34

  • Bester J, Matshailwe C, Pretorius E (2018) Simultaneous presence of hypercoagulation and increased clot lysis time due to IL-1β, IL-6 and IL-8. Cytokine 110:237–242

    Article  CAS  PubMed  Google Scholar 

  • Bhowmick R, Sarwar MH, Dewan SMR, Das A, Das B, Uddin MMN, Islam MS (2014) In vivo analgesic, antipyretic, and anti-inflammatory potential in Swiss albino mice and in vitro thrombolytic activity of hydroalcoholic extract from Litsea glutinosa leaves. Biol Res 47:56

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Biswas M, Sana NK (2014) Isolation, identification and partial purification of lectin from Typhonium trilobatum. IOSR J Pharm Biol Sci 9:41–46

    Google Scholar 

  • Biswas A, Bari M, Roy M, Vadra SK (2010) Inherited folk pharmaceuticals knowledge of tribal people in the Chittagong Hill tracks, Bangladesh. Indian J Tradit Knowl 9:77–89

    Google Scholar 

  • Booyse FM, Pan W, Grenett HE, Parks DA, Darley-Usmar VM, Bradley KM, Tabengwa EM (2007) Mechanism by which alcohol and wine polyphenols affect coronary heart disease risk. Ann Epidemiol 17(5 Suppl):S24–31

    Article  PubMed  Google Scholar 

  • Das HB, Mojumder K, Datta BK, Ray D (2009) Ethnobotanical uses of some plants by Tripuri and Reang tribes of Tripura. Nat Prod Rad 8:172–180

    Google Scholar 

  • Fusconi A, Repetto O, Bona E, Massa N, Gallo C, Dumas-Gaudot E, Berta G (2006) Effect of cadmium on meristem activity and nucleus ploidy in roots of Pisum sativum L. cv, Frisson seedlings. J Environ Exp Bot 58:253–260

    Article  CAS  Google Scholar 

  • Ghani A (1998) Medicinal plants of Bangladesh: chemical constituents and uses. Asiatic Soc Bangladesh, Dhaka

    Google Scholar 

  • Golia E, Limongelli G, Natale F, Fimiani F, Maddaloni V, Pariggiano I, Bianchi R, Crisci M, D’Acierno L, Giordano R, Di Palma G, Conte M, Golino P, Russo MG, Calabrò R, Calabrò P (2014) Inflammation and cardiovascular disease: from pathogenesis to therapeutic target. Curr Atheroscler Rep 16(9):435

    Article  CAS  PubMed  Google Scholar 

  • Haldar KM, Ghosh P, Chandra G (2011) Evaluation of target specific larvicidal activity of the leaf extract of Typhonium trilobatum against Culex quinquefasciatus Say. Asian Pac J Trop Biomed 1:199–203

    Article  Google Scholar 

  • Huerta C, Castellsague J, Varas-Lorenzo C, Garcia Rodriguez LA (2002) Nonsteroidal anti-inflammatory drugs and risk of ARF in the general population. Am J Kidney Dis 45:531–539

    Article  CAS  Google Scholar 

  • Hugenholtz GC, Macrae F, Adelmeijer J, Dulfer S, Porte RJ, Lisman T, Ariëns RA (2016) Procoagulant changes in fibrin clot structure in patients with cirrhosis are associated with oxidative modifications of fibrinogen. J Thromb Haemost 14(5):1054–1066

    Article  CAS  PubMed  Google Scholar 

  • Huq S, Ara H, Hassan A, Alam SS (2007) Comparative karyotype analysis of Typhonium trilobatum L. and its two morphological forms. Jpn Mendel Soc Cytologia 72:233–238

    Google Scholar 

  • Jaradat N, Zaid AN, Hussein F, Zaqzouq M, Aljammal H, Ayesh O (2017) Anti-lipase potential of the organic and aqueous extracts of ten traditional edible and medicinal plants in Palestine; a comparison study with orlistat. Medicines 4(4):89. https://doi.org/10.3390/medicines4040089

    Article  CAS  PubMed Central  Google Scholar 

  • Kandhasamy M, Arunachalam KD (2008) Efficacy of Typhonium trilobatum (L.) Schott tuber extracts on pathogenic bacteria. Egypt J Neurosurg 3:1–7

    Google Scholar 

  • Karaoğlan ES, Albayrak A, Kutlu Z, Bayır Y (2018) Gastroprotective and antioxidant effects of Eremurus spectabilis Bieb. methanol extract and its isolated component isoorientin on indomethacin induced gastric ulcers in rats. Acta Cir Bras 33(7):609–618

    Article  PubMed  Google Scholar 

  • Khan MA (1998) Wildlife biodiversity and its resource potential. In: Gain P (ed) Bangladesh environment: facing the 21st century. Society for Environmental and Human Development (SHED), Dhaka, pp 115–135

    Google Scholar 

  • Khan IN, Habib MR, Rahman MM, Mannan A, Sarker MMI, Hawlader S (2010) Thrombolytic potential of Ocimum sanctum L., Curcuma longa L., Azadirachta indica L. and Anacardium occidentale L. J Basic Clin Pharm 2(3):125–127

    Google Scholar 

  • Khansari PS, Sperlagh B (2012) Inflammation in neurological and psychiatric diseases. Inflammopharmacology 20(3):103–107

    Article  CAS  PubMed  Google Scholar 

  • Kim DC, Kang M, Biswas A, Yang CR, Wang X, Gao JX (2017) Effects of low dose ionizing radiation on DNA damage-caused pathways by reverse-phase protein array and Bayesian networks. J Bioinform Comput Biol 15(2):1750006

    Article  CAS  PubMed  Google Scholar 

  • Konuk M, Liman R, Ciğerci İH (2007) Determination of genotoxic effect of boron on Allium cepa root meristematic cells. Pak J Bot 39(1):73–79

    Google Scholar 

  • Kumar SS, Patra A (2018) Evaluation of phenolic composition, antioxidant, anti-inflammatory and anticancer activities of Polygonatum verticillatum (L.). J Integr Med 16(4):273–282

    Article  Google Scholar 

  • Kumar D, Parida SK, Achrya SN, Patel DD, Thakkar LP, Satyajit (2010) Evaluation of antimicrobial activity of various extracts of Typhonium trilobatum Schott rhizomes. Adv Pharmacol Toxicol 11(3):7–11

    Google Scholar 

  • Li P, Yang Y, Liu M (2011) Xuezhikang, extract of red yeast rice, inhibited tissue factor and hypercoagulable state through suppressing nicotinamide adenine dinucleotide phosphate oxidase and extracellular signal-regulated kinase activation. J Cardiovasc Pharmacol 58(3):307–318

    Article  CAS  PubMed  Google Scholar 

  • Lijnen HR, Van Hoef B, De Cock F, Okada K, Ueshima S, Matsuo O, Collen D (1991) On the mechanism of fibrin-specific plasminogen activation by staphylokinase. J Biol Chem 266(18):11826–11832

    CAS  PubMed  Google Scholar 

  • Majinda RRT (2018) An update of erythrinan alkaloids and their pharmacological activities. Prog Chem Org Nat Prod 107:95–159

    Article  CAS  PubMed  Google Scholar 

  • Mannan A, Kawser MJ, Ahmed AMA, Islam NN, Alam SMA, Emon MAEK, Gupta SD (2011) Assessment of antibacterial, thrombolytic and cytotoxic potential of Cassia alata seed oil. J Appl Pharm Sci 1(9):56–59

    Google Scholar 

  • Marder VJ (1993) Recombinant streptokinase: opportunity for an improved agent. Blood Coagul Fibrinolysis 4(6):1039–1040

    Article  CAS  PubMed  Google Scholar 

  • Melo PS, Arrivetti LOR, Alencar SM, Skibsted LH (2016) Antioxidative and prooxidative effects in food lipids and synergism with α-tocopherol of açaí seed extracts and grape rachis extracts. Food Chem 213:440–449

    Article  CAS  PubMed  Google Scholar 

  • Nicolini FA, Nichols WW, Mehta JL, Saldeen TG, Schofield R, Ross M, Player DW, Pohl GB, Mattsson C (1992) Sustained reflow in dogs with coronary thrombosis with K2P, a novel mutant of tissue-plasminogen activator. J Am Coll Cardiol 20(1):228–235

    Article  CAS  PubMed  Google Scholar 

  • Ogston D, Lea AG, Langhorne P, Wilson SB (1985) The influence of the polyphenols of cider on plasmin and plasminogen activators. Br J Haematol 60(4):705–713

    Article  CAS  PubMed  Google Scholar 

  • Olatunji LA, Michael OS, Adeyanju OA, Areola ED, Soladoye AO (2017) Anti-inflammatory and antithrombotic effects of nicotine exposure in oral contraceptive-induced insulin resistance are glucocorticoid-independent. Pharmacol Rep 69(3):512–519

    Article  CAS  PubMed  Google Scholar 

  • Pistollato F, Cano S, Elio I, Vergara M, Giampieri F, Battino M (2015) Plant-based and plant-rich diet patterns during gestation: beneficial effects and possible shortcomings. Adv Nutr 6(5):581–591

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Prasad S, Kashyap RS, Deopujari JY, Purohit HJ, Taori GM, Daginawala HF (2006) Development of an in vitro model to study clot lysis activity of thrombolytic drugs. Thromb J 4:14

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Qin R, Wang C, Chen D, Björn LO, Li S (2015) Copper-induced root growth inhibition of Allium cepa var. agrogarum L. involves disturbances in cell division and DNA damage. Environ Toxicol Chem 34:1045–1055

    Article  CAS  PubMed  Google Scholar 

  • Rahman F (1999) Bangladesh risk assessment and management bio safety capacity building initiative of IUCN Asia. Working paper, Ministry of Environment and Forest, Dhaka, Bangladesh

  • Rahmatullah M, Rahman L, Rehana F, Kalpana MA, Khatun MA, Jahan R (2010) A scientific evaluation of medicinal plants used in the folk medicinal system of five villages in Narsinghdi District, Bangladesh. Am-Eurasian J Sustain Agric 4:55–64

    Google Scholar 

  • Ray J, Prusty A, Maharana L (2015) Study of antidiabetic activity of Typhonium trilobatum in glucose loaded & alloxan induced hyperglycemic and normoglycemic rats. Pharmacophore 6(5):233–242

    Google Scholar 

  • Roy SK, Mishra PK, Nandy S, Datta R, Chakraborty B (2012a) Potential wound healing activity of the different extract of Typhonium trilobatum in albino rats. Asian Pac J Trop Biomed 2:S1477–S1486

    Article  Google Scholar 

  • Roy SK, Mishra PK, Nandy S, Patel VK (2012b) Assessment of antimicrobial activity of Typhonium trilobatum plant. Int J Pharm 2(3):625–630

    Google Scholar 

  • Sambola A, Ruiz-Meana M, Barba I, Del Blanco BG, Barrabés JA, Lip GY, Vilardosa Ú, Sansaloni S, Rello P, García-Dorado D (2017) Glycative and oxidative stress are associated with altered thrombus composition in diabetic patients with ST-elevation myocardial infarction. Int J Cardiol 243:9–14

    Article  PubMed  Google Scholar 

  • Sdayria J, Rjeibi I, Feriani A, Ncib S, Bouguerra W, Hfaiedh N, Elfeki A, Allagui MS (2018) Chemical composition and antioxidant, analgesic, and anti-inflammatory effects of methanolic extract of Euphorbia retusa in mice. Pain Res Manag 48:38. https://doi.org/10.1155/2018/4838413

    Article  Google Scholar 

  • Sen S, Chakraborty R, Rekha B, Revathi D, Ayyanna SC, Hemalatha G, Kumar Reddy GA, Hyndavi S, Ikhyatha Babu PJ, Prakash PR, Sridhar C (2013) Anti-inflammatory, analgesic, and antioxidant activities of Pisonia aculeata: folk medicinal use to scientific approach. Pharm Biol 51(4):426–432

    Article  CAS  PubMed  Google Scholar 

  • Seth CS, Chaturvedi PK, Misra V (2007) Toxic effect of arsenate and cadmium alone and in combination on Giant Duckweed (Spirodela polyrrhiza L.) in response to its accumulation. Environ Toxicol 22:539–549

    Article  CAS  PubMed  Google Scholar 

  • Sevcovicova A, Bodnarova K, Loderer D, Imreova P, Galova E, Miadokova E (2014) Dual activities of emodin–DNA protectivity vs mutagenicity. Neuro Endocrinol Lett 35:149–154

    CAS  PubMed  Google Scholar 

  • Shafa F, Shahriar M, Dain FA, Opo M, Akhter R, Hossain MM, Choudhury N (2016) Characterization of phytoconstituents, in vitro antioxidant activity and pharmacological investigation of the root extract of Typhonium trilobatum. Int J Pharm Sci Res 7(4):1694–1704

    CAS  Google Scholar 

  • Shahidullah AKM (2007) The role of medicinal plants in livelihood improvement and ecological sustainability in Bangladesh, University of Manitoba, p 34. http://hdl.handle.net/1993/29464

  • Shahriar M, Tithi NA, Akhter R, Kamal S, Narjish SN, Bhuiyan MA (2015) Phytochemical and Pharmacological investigation of the Typhonium trilobatum (L.) schott. World J Pharm Res 4(2):167–188

    Google Scholar 

  • Shanmugam S, Kalaiselvan PM, Selvalumar SK, Rajendran K (2011) Ethnomedicinal plants used to cure diarrhea and dysentery in Sivagangai district of Tamil Nadu, India. Int J Res Ayurveda Pharm 2:991–994

    Google Scholar 

  • Sharma R, Kumar R, Kodwani R, Kapoor S, Khare A, Bansal R, Khurana S, Singh S, Thomas J, Roy B, Phartyal R, Saluja S, Kumar S (2015) A review on mechanisms of anti tumor activity of chalcones. Anticancer Agents Med Chem 16(2):200–211

    Article  CAS  PubMed  Google Scholar 

  • Shih SC, Chang CW (2007) Nonsteroidal anti-inflammatory drug related gastrointestinal bleeding in the elderly. Int J Gerontol 1:40–45

    Article  Google Scholar 

  • Sofowora A (1993) Medicinal plants and traditional medicine in Africa. Spectrum Books, Ibadan, p 150

    Google Scholar 

  • Sreejith G, Latha PG, Shine VJ, Anuja GI, Suja SR, Sini S, Shyama S, Pradeep S, Shikha P, Rajasekharan S (2010) Anti-allergic, anti-inflammatory and anti-lipidperoxidant effects of Cassia occidentalis Linn. Indian J Exp Biol 48(5):494–498

    CAS  PubMed  Google Scholar 

  • Thomas MC (2000) Diuretics, ACE inhibitors and NSAIDs—the triple whammy. J Med 172:184–185

    CAS  Google Scholar 

  • Trease GE (1992) A text book of pharmacognosy, 13th edn. Baillier, Tindal and Caussel, London

    Google Scholar 

  • Ullah HMA, Zaman S, Juhara F, Akter L, Tareq SM, Masum EH, Bhattacharjee R (2014) Evaluation of antinociceptive, in vivo & in vitro anti-inflammatory activity of ethanolic extract of Curcuma zedoaria rhizome. BMC Complement Altern Med 14:346

    Article  PubMed  PubMed Central  Google Scholar 

  • Wallace JL (2001) Pathogenesis of NSAID induced gastroduodenal mucosal injury. Best Pract Res Clin Gastroenterol 15:691–703

    Article  CAS  PubMed  Google Scholar 

  • Webster PL, Macleod RD (1996) The root apical meristem and its margin. In: Waishel Y, Eshel A, Kafkafi U (eds) Plant roots. The hidden half, 2nd edn. Marcel Dekker, New York, pp 51–76

    Google Scholar 

  • World Health Organization (WHO) (1990a) Medicinal plants in Viet Nam. WHO Regional Publications, WHO Regional Office for the Western Pacific, Manila, p 3

    Google Scholar 

  • World Health Organization (WHO) (1990b) Medicinal plants in Viet Nam. WHO Regional Publications, Western Pacific Series No. 3, WHO Regional Office for the Western Pacific, Manila, ISBN 92 9061 101 4

  • Wu DH, Shi GY, Chuang WJ, Hsu JM, Young KC, Chang CW, Wu HL (2001) Coiled coil region of streptokinase gamma-domain is essential for plasminogen activation. J Biol Chem 276(18):15025–15033

    Article  CAS  PubMed  Google Scholar 

  • Yusuf M, Chowdhury JU, Wahab MA, Begum J (1994) Medicinal Plants of Bangladesh. Bangladesh Council of Scientific and Industrial Research, Dhaka, p 34

    Google Scholar 

  • Zhou J, Chao G, Li Y, Wu M, Zhong S, Feng Z (2016) Activation of NRF2/ARE by isosilybin alleviates Aβ25-35-induced oxidative stress injury in HT-22 cells. Neurosci Lett 632:92–97

    Article  CAS  PubMed  Google Scholar 

  • Zhou Y, Sun Y, Li P, Qin G, Cheng Q, Liu Y, Chen Y, Wang G (2018) Monoside antagonizes triptolide-induced hepatocyte apoptosis via the anti-oxidative stress pathway. Nan Fang Yi Ke Da Xue Xue Bao 38(8):949–955

    CAS  PubMed  Google Scholar 

Download references

Acknowledgements

We are thankful to the Department of Pharmacy, Banghabondhu Sheikh Mujibur Rahman Science and Technology University for providing the laboratory facilities to conduct this study. We are also owed to the Department for Management of Science and Technology Development & Faculty of Pharmacy, Ton Duc Thang University, Vietnam for funding this study.

Author information

Authors and Affiliations

  1. Department of Pharmacy, Banghabondhu Sheikh Mujibur Rahman Science and Technology University, Gopalganj, 8100, Bangladesh

    Tania Parvin, Mim Binti Akhlas, Fatema Khatun, Anzuman Akter, Md. Al Amin & Razina Rouf

  2. Department for Management of Science and Technology Development, Faculty of Pharmacy, Ton Duc Thang University, Ho Chi Minh City, 700000, Vietnam

    Muhammad Torequl Islam

  3. Faculty of Pharmacy, Ton Duc Thang University, Ho Chi Minh City, 700000, Vietnam

    Muhammad Torequl Islam

Authors
  1. Tania Parvin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Mim Binti Akhlas
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Fatema Khatun
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Anzuman Akter
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Md. Al Amin
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Muhammad Torequl Islam
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Razina Rouf
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Muhammad Torequl Islam.

Ethics declarations

Ethical statement

This study was approved by the Ethical Committee under the Department of Pharmacy, Bangabandhu Sheikh. Mujibur Rahman Science and Technology University (BSMRSTU) (Approval No. 2014/0109031).

Conflicts of interest

Tania Parvin has no conflict of interest. Mim Binti Akhlas has no conflict of interest. Fatema Khatun has no conflict of interest. Anzuman Akter has no conflict of interest. Md. Al Amin has no conflict of interest. Muhammad Torequl Islam has no conflict of interest. Razina Rouf has no conflict of interest.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Parvin, T., Akhlas, M.B., Khatun, F. et al. Phytochemical screening and evaluation of pharmacological activities of aqueous extract of Typhonium trilobatum (L.) Schott. Orient Pharm Exp Med 19, 445–454 (2019). https://doi.org/10.1007/s13596-019-00382-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13596-019-00382-0

Keywords

Search

Navigation