Abstract
This study was to prepare a novel in situ gel system for nasal delivery of poly-herbal extracts and study its efficacy on the allergic rhinitis model. The main purpose is to compare the efficacy of poly-herbal nasal drops and in situ gel. A temperature depended in situ gel was developed and characterized with gellan gum as a carrier. The system was stable kept at 40 ± 2 °C for 6 months, and the micrographic results showed that in situ gel was safety without mucous irritation when given daily for 26 days to mice with allergic rhinitis that the effect of drops for the treatment. Extracts in gellan gum produced obviously effect on allergic rhinitis at the doses of 20 g/body following intranasal administration, and the efficacy was significantly superior to that of the drops (p < 0.01). This study uses Kruskal–Wallis test analysis of variance (ANOVA) followed by Dunn’s test for the first time to develop, optimize the formulation and assess factors affecting the critical quality attributes. Histopathological study of the nasal mucosa suggested that the formulation was safe for nasal administration. The statistical difference in absolute bioavailability between drop and in situ gel by nasal route suggested that in situ gel had almost 21% increase in bioavailability for Moringa oleifera and for Embelia Ribes in combination there was a 16% increase over drop formulations. Thus the situ gel system is a promising approach for the intranasal delivery of polyherbal extracts for the therapeutic effect improvement.
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Alving K, Malinovschi A (2010) Basic aspects of exhaled nitric oxide. Exhaled Biomark 49:1–31
Bahekar PC, Shah JH, Ayer UB, Mandhane SN, Thennati R (2008) Validation of guinea pig model of allergic rhinitis by oral and topical drugs. Int Immunopharmacol 8:1540–1551
Bielory L, Lupoli K (1999) Herbal interventions in asthma and allergy. J Asthma 36:1–65
Church MK, Levi-Schaffer F (1997) The human mast cell. J Allergy Clin Immunol 99:155–160
Ciprandi G, Signori A, Cirillo I (2011) Relationship between bronchial hyperreactivity and bronchodilation in patients with allergic rhinitis. Ann Allergy Asthma Immunol 106(6):460–466
Fulkerson PC, Rothenberg ME (2013) Targeting eosinophils in allergy, inflammation and beyond. Nat Rev Drug Discov 12:117–129
Gonzalez-Segovia R, Quintanar JL, Salinas E, Ceballos-Salazar R, Aviles-Jimenez F, Torres-Lopez J (2008) Effect of the flavonoid quercetin on inflammation and lipid peroxidation induced by Helicobacter pylori in gastric mucosa of guinea pig. J Gastroenterol 43:441–447
Hebestreit H, Dibbert B, Balatti I et al (1998) Disruption of fas receptor signaling by nitric oxide in eosinophils. J Exp Med 187:415–425
Hu GR, Shen ZY, Shi SZ et al (1983) Effect of the kidney reinforcing regimen on serum IgE and T cell subsets in the treatment of asthma. J Tradit Chin Med 5:33–36
Hu GR, Shen ZY, Xu DS et al (1987) Lymphocyte adrenergic receptor function in asthmatic subjects with the treatment of Wen Yang Pill. Chun Kuo Chung His I Chieh Ho Tsa Chih 7:71–74
Joshi SS, Kuszynski CA, Bagchi D (2001) The cellular and molecular basis of health benefits of grape seed proanthocyanidin extract. Curr Pharm Biotechnol 2:187–200
Jung HW, Jung JK, Cho CW, Kang JS, Park YK (2012) Antiallergic effect of KOB03, a polyherbal medicine, on mast cell-mediated allergic responses in ovalbumin-induced allergic rhinitis mouse and human mast cells. J Ethnopharmacol 142(3):684–693
Kimata M, Inagaki N, Nagai H (2000) Effects of luteolin and other flavonoids on IgE-mediated allergic reactions. Planta Med 66:25–29
Leung AK, Hon KL (2008) Seasonal allergic rhinitis. Recent Pat Inflamm Allergy Drug Discov 2:175–185
Meltzer EO (2013) Pharmacotherapeutic strategies for allergic rhinitis: matching treatment to symptoms, disease progression, and associated conditions. Allergy Asthma Proc 34:301–311
Miranda KM, Espey MG, Wink DA (2001) A rapid, simple spectrophotometric method for simultaneous detection of nitrate and nitrite. Nitric Oxide 5:62–71
Nakano N, Nishiyama C, Tokura T et al (2008) Procyanidin C1 from apple extracts inhibits Fc epsilon RI-mediated mast cell activation. Int Arch Allergy Immunol 147:213–221
Schmolka IR (1972) Artificial skin: preparation and properties of Pluronic F-127 gels for treatment of burns. J Biomed Mater Res 6:571–582
Settipane RA, Schwindt C (2013) Allergic rhinitis. Am J Rhinol Allergy 27(3_suppl):S52–S5
Shen Y, Jia LN, Honma N, Hosono T, Ariga T, Seki T (2012) Beneficial effects of cinnamon on the metabolic syndrome, inflammation, and pain, and mechanisms underlying these effects a review. J Tradit Complement Med 2:27–32
Shii D, Nakagawa S, Yoshimi M, Katsuta O, Oda T, Nakamura M (2010) Inhibitory effects of cyclosporine an eye drops on symptoms in late phase and delayed-type reactions in allergic conjunctivitis models. Biol Pharm Bull 33:1314–1318
Srivastava A, Greenspan P, Hartle DK, Hargrove JL, Amarowicz R, Pegg RB (2010) Antioxidant and antiinflammatory activities of polyphenolics from Southeastern U.S. range blackberry cultivars. J Agric Food Chem 58:6102–6109
Tsunematsu M, Yamaji T, Kozutsumi D, Murakami R, Kimura S, Kino K (2007) Establishment of an allergic rhinitis model in mice for the evaluation of nasal symptoms. Life Sci 80:1388–1394
Wang H, Zhang J, Gao C, Zhu Y, Wang C, Zheng W (2007) Topical levamisole hydrochloride therapy attenuates experimental murine allergic rhinitis. Eur J Pharmacol 577:162–169
Yamasaki ML, Sasaki K, Mizutani N et al (2001) Pharmacological characterization of the leukocyte kinetics after intranasal antigen challenge in a guinea pig model of allergic rhinitis. Inflamm Res 50:474–482
Yun MY, Yang JH, Kim DK, Cheong KJ, Song HH, Kim DH, Cheong KJ, Kim YI, Shin SC (2010) Therapeutic effects of Baicalein on atopic dermatitis-like skin lesions of NC/Nga mice induced by dermatophagoides pteronyssinus. Int Immunopharmacol 10:1142–1148
Zanardo RC, Costa E, Ferreira HH et al (1997) Pharmacological and immunohistochemical evidence for a functional nitric oxide synthase system in rat peritoneal eosinophils. Proc Natl Acad Sci USA 94:14111–14114
Ziment I, Tashkin DP (2000) Alternative medicine for allergy and asthma. J Allergy Clin Immun 106(4):603–614
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All the studies were performed at Amity Institute of Pharmacy, Amity University Lucknow campus Uttar Pradesh. We are highly thankful to the institute for proving us the platform to conduct our studies.
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This article does not contain any studies with human participants performed by any of the authors. Animal handling and experimental procedures were performed according to the guideline for Care and Use of Animals Laboratory (No: AIP/IAEC/06/2017) was approved by the Institutional Animal Ethics Committee.
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This manuscript described has not been published before; not under consideration for publication anywhere else; and has been approved by all co-authors.
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Srivastava, R., Srivastava, S. & Singh, S.P. Comparative study of anti-allergic activity of two poly-herbal formulations in ova-challenged allergic rhinitis mice model. Orient Pharm Exp Med 19, 37–47 (2019). https://doi.org/10.1007/s13596-018-0345-7
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DOI: https://doi.org/10.1007/s13596-018-0345-7