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Phytochemical analysis and antioxidant potential of the crude extract of Allium oschaninii scape

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The present study focuses on the antioxidant potential of aqueous and ethanolic extracts of the scape of Allium oschaninii. Phytochemical analysis and total phenolic content for both the extracts and Total flavonoid content for ethanolic extract were determined. Anti-microbial activity of different concentrations of both the extracts against Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, and Bacillus cereus was investigated. The total antioxidant power (TAP), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH) and Ferric reducing ability of plasma (FRAP) assays were carried out. IC50 values of aqueous and ethanolic extract to scavenge ABTS and DPPH free radical were determined as 0.2524, 0.5467 and 0.6155, 0.9591 (mg/mL) respectively. FRAP and TAP results of both aqueous and ethanolic extracts were determined to be 2530.972 and 1199.86 (µmol of Fe2+ per 1000 µg of extract) and 84.13 ± 5.51 and 141.88 ± 40.49 (equivalent mg of ferulic acid per 150 mg dried sample) respectively. These results signify the antioxidant power potential of the scape of A.oschaninii.

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  • Aoyama S, Yamamoto Y (2007) Antioxidant activity and flavonoid content of Welsh onion (Allium fistulosum) and the effect of thermal treatment. Food Sci Technol Res 13:67–72

    Article  CAS  Google Scholar 

  • Arora DS, Kaur J (1999) Antimicrobial activity of spices. Int J Antimicrob Ag 12:257–262

    Article  CAS  Google Scholar 

  • Barile E, Bonanomi G, Antignani V, Zolfaghari B, Sajjadi E, Scala F, Lanzotti V (2007) Saponins from Allium minutiflorum with antifungal activity. Phytochemistry 68:593–603

    Article  Google Scholar 

  • Benkeblia N (2004) Antimicrobial activity of essential oil extracts of various onions (Allium cepa) and garlic (Allium sativum). LWT-Food Sci Technol 37:263–268

    Article  CAS  Google Scholar 

  • Chakraborty P, Kumar S, Dutta D, Gupta V (2009) Role of antioxidants in common health diseases. Research J Pharm Tech 2:238–244

    CAS  Google Scholar 

  • Curcic MG et al (2012) Biological effects, total phenolic content and flavonoid concentrations of fragrant yellow onion (Allium flavum L.). Med Chem 8:46–51

    Article  CAS  Google Scholar 

  • Dini I, Tenore GC, Dini A (2008) Chemical composition, nutritional value and antioxidant properties of Allium caepa L. Var. tropeana (red onion) seeds. Food Chem 107:613–621

    Article  CAS  Google Scholar 

  • Donnell G, Gibbons S (2007) Antibacterial activity of two canthin -6-one alkaloids from Allium neapolitanum. Phytother Res. 21:653–657

    Article  Google Scholar 

  • Doughari JH (2012) Phytochemicals: extraction methods, basic structures and mode of action as potential chemotherapeutic agents. In: Phytochemicals-A global perspective of their role in nutrition and health. InTech, Croatia, pp 1–32

  • Edeoga H, Okwu D, Mbaebie B (2005) Phytochemical constituents of some Nigerian medicinal plants. Afr J Biotechnol 4:685–688

    Article  CAS  Google Scholar 

  • Fidrianny I, Permatasari L, Wirasutisna KR (2013) Antioxidant activities from various bulbs extracts of three kinds allium using DPPH, ABTS assays and correlation with total phenolic, flavonoid, carotenoid content. Int J Res Pharm Sci 4:438–444

    Google Scholar 

  • Guru S, Manoj M, Vasanthi M, Achary A (2015) Antioxidant and free radical scavenging potential of crude sulphated polysaccharides from Turbinaria ornata. Biologia 70:27–33

    Google Scholar 

  • Issa M, Karabet F, Aljoubbeh M (2013) Total polypheonls, flavonoid content, kaempferol concentration and antioxidant activity of two onion Syrian (Spring And White). Int J ChemTech Res 5:2375–2380

    CAS  Google Scholar 

  • Jadon R, Dixit S (2014) Phytochemical extraction and antimicrobial activity of some medicinal plants on different microbial strains. J Med Plants Stud 2:58–63

    Google Scholar 

  • Kumar S (2011) Free radicals and antioxidants: human and food system. Adv Appl Sci Res 2:129–135

    Google Scholar 

  • Laghari AQ, Memon S, Nelofar A, Laghari AH (2011) Extraction, identification and antioxidative properties of the flavonoid-rich fractions from leaves and flowers of Cassia angustifolia. Am J Anal Chem 2:871

    Article  CAS  Google Scholar 

  • Lu X, Wang J, Al-Qadiri HM, Ross CF, Powers JR, Tang J, Rasco BA (2011) Determination of total phenolic content and antioxidant capacity of onion (Allium cepa) and shallot (Allium oschaninii) using infrared spectroscopy. Food Chem 129:637–644

    Article  CAS  Google Scholar 

  • Ma X et al (2011) Polyphenolic compounds and antioxidant properties in mango fruits. Sci Hortic-Amst 129:102–107

    Article  CAS  Google Scholar 

  • Mikaila P, Maadirad S, Moloudizargari M, Aghajanshakeri S, Sarahroodi S (2013) Therapeutic uses and pharmacological properties of garlic, shallot, and their biologically active compounds. Iran J Basic Med Sci 16:1031–1048

    Google Scholar 

  • Mollavali M, Bolandnazar S, Nazemieh H, Zare F, Aliasgharzad N (2015) The effect of mycorrhizal fungi on antioxidant activity of various cultivars of onion (Allium cepa L). Int J Biosci 6:66–79

    Google Scholar 

  • Mskhiladze L, Legault J, Lavoie S, Mshvildadze V, Kuchukhidze J, Elias R, Pichette A (2008) Cytotoxic steroidal saponins from the flowers of Allium leucanthum. Molecules 13:2925–2934

    Article  CAS  Google Scholar 

  • Nile SH, Park SW (2013) Total Phenolics, antioxidant and xanthine oxidase inhibitory activity of three colored onions (Allium cepa L.). Front Life Sci 7:224–228

    Article  CAS  Google Scholar 

  • Parvu M, Toiu A, Vlase L, Parvu EA (2013) Determination of some polyphenolic compounds from Allium species by HPLC-UV-MS. Nat Prod Res 24:1318–1324

    Article  Google Scholar 

  • Rouis-Soussi LS, Boughelleb-M’Hamdi N, El Ayeb-Zakhama A, Flamini G, Jannet HB, Harzallah-Skhiri F (2014) Phytochemicals, antioxidant and antifungal activities of Allium roseum var. grandiflorum subvar. typicum Regel. S Afr J Bot 91:63–70

    Article  Google Scholar 

  • Sahu M, Vermaand D, Harris K (2014) phytochemical analysis of the leaf, stem and seed extracts of Cajanus cajan L (Dicotyledoneae: Fabaceae). world. J Pharm Pharm Sci 3:694–733

    Google Scholar 

  • Saxena M, Saxena J, Pradhan A (2012) Flavonoids and phenolic acids as antioxidants in plants and human health. Int J Pharm Sci Rev Res 16:130–134

    CAS  Google Scholar 

  • Shenoy C, Patil M, Kumar R, Patil S (2009) Preliminary phytochemical investigation and wound healing activity of Allium cepa Linn (Liliaceae). Int J Pharm Pharm Sci 2:167–175

    Google Scholar 

  • Srivastava M, Tiwari R, Sharma N (2013) Assessment of phenol and flavonoid content in the plant materials. J New Biol Rep 2:163–166

    Google Scholar 

  • Ugochukwu SC, Uche A, Ifeanyi O (2013) Preliminary phytochemical screening of different solvent extracts of stem bark and roots of Dennetia tripetala G. Baker. Asian J Plant Sci Res 3:10–13

    Google Scholar 

  • Wang B-S, Lin S-S, Hsiao W-C, Fan J-J, Fuh L-F, Duh P-D (2006) Protective effects of an aqueous extract of Welsh onion green leaves on oxidative damage of reactive oxygen and nitrogen species. Food Chem 98:149–157

    Article  CAS  Google Scholar 

  • Weisburger J (1999) Mechanisms of action of antioxidants as exemplified in vegetables, tomatoes and tea. Food Chem Toxicol 37:943–948

    Article  CAS  Google Scholar 

  • Yousufi K (2012) To study antibacterial activity of Allium sativum, Zingiber officinale and Allium cepa by Kirby-Bauer method. IOSR J Pharm Biol Sci 4:6–8

    Google Scholar 

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Laboratory facilities for this project were provided by the Department of Biotechnology, Kamaraj College of Engineering and Technology. The study was not supported by any source of funding.

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Correspondence to Rengesh Balakrishnan.

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This manuscript described has not been published before; not under consideration for publication anywhere else; and has been approved by all co-authors.

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Alagarsamy, S., Chellappan, P., Jesuraj, M.T. et al. Phytochemical analysis and antioxidant potential of the crude extract of Allium oschaninii scape. Orient Pharm Exp Med 18, 309–316 (2018).

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