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Rosa damascena Mill. L. attenuates myocardial lysosomal membrane destabilization in isoproterenol induced oxidative stress

Abstract

Lysosomal membrane damage suggests that it would be the major contributing factor of myocardial infarction followed by necrosis. The present investigation was carried out on the defensive effect of ethanolic extract of Rosa damascena Mill. L. extract on alterations in myocardial lysosomal enzyme activity and membrane bound Na+/K+ ATPases against isoproterenol induced myocardial infarction in rats. On treatment with isoproterenol (100 mg/kg body weight) for two consecutive days, animals have shown a significant increase in serum creatinine kinase-MB, lactate dehydrogenase and lysosomal enzyme activity; whereas membrane bound Na+/K+ ATPases and tissue antioxidants were decreased. Pre-treatment with Rosa damascena Mill. L. extracts 200 and 400 mg/kg body weight respectively, for 28 days, significantly prevented the alterations and restored the CK-MB, LDH, tissue antioxidants and lysosomal enzyme activity to near-normal status in rats. These findings demonstrate that Rosa damascena could preserve lysosomal integrity through increasing the antioxidant enzyme levels and membrane bound Na+/K+ ATPases integrity. The cardio protective activity of Rosa damascena was further supported by histopathological examination of heart sections. Hence all these findings confirm cardio protective potential of ethanolic extract of Rosa damascena.

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Acknowledgements

Authors are thankful to principal and management of Sri Padmavathi School of Pharmacy for providing the laboratory facilities and ethical committee approval.

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Correspondence to Chitikela P. Pullaiah.

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Ethical Statement

All the experimental protocols were described and approved by the Institutional Animal Ethical Committee of Sri Padmavathi School of Pharmacy, Tirupati.

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All the authors declare that they have no conflicts of interest.

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Pullaiah, C.P., Narasimha Kumar, G.V., Jyothsna, K. et al. Rosa damascena Mill. L. attenuates myocardial lysosomal membrane destabilization in isoproterenol induced oxidative stress. Orient Pharm Exp Med 17, 373–380 (2017). https://doi.org/10.1007/s13596-017-0290-x

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Keywords

  • Antioxidant
  • Cardioprotection
  • Isoproterenol
  • Oxidative stress
  • Lysosomal enzyme