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Lasianthera Africana leaves inhibits α-amylase α-glucosidase, angiotensin-I converting enzyme activities and Fe2+-induced oxidative damage in pancreas and kidney homogenates

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Abstract

Lasianthera africana is a dark green leafy vegetable commonly consumed as food and used for treatment of several human ailments in Nigeria most especially south-east. This study investigated the phenolic composition and effects of methanol extract from L. africana leaves on enzymes linked to type-2 diabetes (α-amylase and α-glucosidase) and hypertension [angiotensin-1-converting enzymes (ACE)] as well as Fe2+-induced lipid peroxidation in rat’s pancreas and kidney homogenates were evaluated. Phenolic contents and constituents were also determined using colorimetric methods and high performance liquid chromatography-diode array detector (HPLC-DAD) respectively. Our findings revealed that the extract inhibited α-amylase, α-glucosidase and ACE activities with IC50 values of 0.192 and 0.21 mg/mL, and 57.06 μg/mL respectively. The extract also inhibited Fe2+-induced lipid peroxidation in rats’ pancreas and kidney homogenates. Our findings revealed that the extract is rich in phenolic acids such as caffeic acid (59.97 mg/g), chlorogenic acid (58.23 mg/g) and ellagic acid (31.16 mg/g) as well as flavonoids such as quercetin (42.98 mg/g). These results give a clue to the potential use of L. africana leaves as an alternative agent for the control of glucose absorption and lowering of blood pressure in diabetic patients with hypertension.

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Acknowledgements

Authors acknowledge every member of Functional Food and Nutracetical Laboratory Unit of Biochemistry departement, Federal Univervisty of Technology, Akure, where this work was carried out.

Author contributions

SAS, GO and SIO designed the study, participated in laboratory works, read the first draft and approved the final draft. SIO, TAO and AAA carried out sample preparation, laboratory experiment, collection and interpretation of data. AAB carried out the HPLC-DAD analysis.

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Authors and Affiliations

  1. Department of Biochemistry, Adekunle Ajasin University, P.M.B. 01, Akungba Akoko, Ondo State, Nigeria

    Sidiqat A. Shodehinde

  2. Functional Foods and Nutraceuticals Unit, Department of Biochemistry, Federal University of Technology, Akure, P.M.B. 704, Akure, 340001, Nigeria

    Sunday I. Oyeleye, Adeniyi A. Adebayo & Ganiyu Oboh

  3. Department of Biomedical Technology, Federal University of Technology, Akure, P.M.B. 704, Akure, 340001, Nigeria

    Sunday I. Oyeleye

  4. Nutrition and Toxicology Division, Food Technology Department, Federal Institute of Industrial Research, Oshodi, Lagos, 100261, Nigeria

    Tosin A. Olasehinde

  5. Phytochemical Research Laboratory, Department of Industrial Pharmacy, Federal University of Santa Maria, Build 26, room 1115, Santa Maria, CEP 97105-900, Brazil

    Aline A. Boligon

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  1. Sidiqat A. Shodehinde
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  2. Sunday I. Oyeleye
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  3. Tosin A. Olasehinde
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Correspondence to Sidiqat A. Shodehinde or Ganiyu Oboh.

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Ethical approval was obtained from the Animal ethical committee of the School of Science Federal University of Technology Akure with Approval No FUTA/SOS/1399. Handling of the animals was in accordance with the Guide for Care and Use of Laboratory Animals prepared by the National Academy of Science which was published by the National Institute of Health (USA)

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Shodehinde, S.A., Oyeleye, S.I., Olasehinde, T.A. et al. Lasianthera Africana leaves inhibits α-amylase α-glucosidase, angiotensin-I converting enzyme activities and Fe2+-induced oxidative damage in pancreas and kidney homogenates. Orient Pharm Exp Med 17, 41–49 (2017). https://doi.org/10.1007/s13596-017-0256-z

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