Immunomodulatory properties and biomarkers characterization of novel Withania somnifera based formulation supplemented with minerals in Sprague Dawley rats

  • Mahendra Kumar Trivedi
  • Mayank Gangwar
  • Sambhu Charan Mondal
  • Snehasis JanaEmail author
Research Article


Herbomineral formulations have been accepted world-wide due to its significant therapeutic outcomes. The study aimed to evaluate the immunomodulatory potential of novel herbomineral formulation (TEBEH) on Sprague Dawley rats using cyclophosphamide-induced immunosuppression. Major immune related parameters were monitored such haemagglutination assay, delayed type hypersensitivity reaction, cellular immune response (CD4+ and CD8+), IgG and IgM, hematological analysis, antioxidant profile, and estimation of major lipid biomarkers. The study results suggest herbomineral formulation significantly (p ≤ 0.05) improved the concentration of primary and secondary titre by 41.18 and 68%. However, the rat paw edema at 24 h showed increased edema by 77.78% (p ≤ 0.01). Herbomineral formulation exhibited increase in the CD4+ and CD8+ counts in animals by 28.03 and 60.70%, respectively. In addition, the concentrations of IgM and IgG were improved by 14.29 and 8.26% respectively. Besides, other supportive parameter such as hematology and antioxidant profile was significantly (p ≤ 0.001) improved, while free radical, lipid peroxidation was decreased. Moreover, the major lipid biomarkers were improved such as decreased triglycerides, LDL and VLDL by 15.88, 3.65, and 16.01%, respectively. Overall, the promising results of this study indicated that the herbomineral formulation, TEBEH can be employed to improve the cellular and humoral immunity along with improved hematological, antioxidant, and lipid profile.

Graphical abstract


Immunopotentiation Herbomineral product Cellular and humoral immunity Hematological parameters Lipid profile Antioxidant profile 



The authors extend their sincere thanks and grateful to Dabur Research Foundation, Ghaziabad, India for providing the facilities and support that enabled the successful completion of the work. Authors gratefully acknowledged to GVK Biosciences Pvt. Ltd., Hyderabad, India for their support in chemical characterization of ashwagandha root extract.

Compliance with ethical standards

Ethical Statement

All the animals used in this experiment were approved by Animal Ethics Committee approved protocol (IAEC/36/394) and the husbandry conditions maintained as per CPCSEA recommendations.

Conflicts of Interest

The authors declared no conflicts of interest in this work.


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Copyright information

© Institute of Korean Medicine, Kyung Hee University and Springer Science+Business Media Dordrecht 2017

Authors and Affiliations

  • Mahendra Kumar Trivedi
    • 1
  • Mayank Gangwar
    • 2
  • Sambhu Charan Mondal
    • 2
  • Snehasis Jana
    • 2
    Email author
  1. 1.Trivedi Global, Inc.HendersonUSA
  2. 2.Trivedi Science Research Laboratory Pvt. Ltd.BhopalIndia

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