Antioxidant, genoprotective and immunomodulatory potential of Vitex negundo leaves in experimental arthritis
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Vitex negundo is a medicinal plant used in Indian and folklore medicines to cure various ailments including arthritis. In the present study, the hydroethanolic extract of V. negundo leaves (VNE) were evaluated for antioxidant, genoprotective and immunomodulatory activity in Freund’s complete adjuvant (FCA) induced arthritis. VNE was evaluated for in vitro antioxidant activity using various parameters including plasmid nicking assay. VNE (200 mg/kg body weight) was orally administered to FCA induced arthritic rats to evaluate its genoprotective effects on peripheral blood leukocytes using the alkaline comet assay. Furthermore, the effects of VNE treatment on serum proinflammatory mediators such as tumor necrosis factor-α (TNF-α) and interleukin -1α (IL-1α) and the hematological parameters such as total RBC count, hematocrit and hemoglobin were measured in arthritic rats. The results showed that VNE exhibited potential in vitro antioxidant and DNA protecting activities in a concentration dependent manner. The phytoconstituents of VNE showed a strong and positive correlation with the antioxidant properties. DNA damage in the peripheral blood leukocytes of arthritic animals was significantly decreased after VNE treatment. VNE showed inhibition of serum proinflammatory cytokines (TNF-α and IL-1α) however, the inhibition was statistically not-significant. The arthritic animals showed significant increase in hemolysis, which was restored by VNE treatment. The results of the present study indicate that VNE may be a source of antioxidant, genoprotective with immunomodulatory activity, which may be attributed to its antioxidant phytoconstituents.
KeywordsVitex negundo Antioxidant Plasmid Freund’s adjuvant Comet assay TNF-α IL-1α
Compliance with ethical standards
All the experiments were ap proved and conducted as per the guidelines of the Institutional Animal Ethics Committee (IAEC) (No. Biochem /01/2013–14).
Conflict of Interest
The authors declare that there are no conflicts of interest.
- Halliwell B, Gutteridge JMC (1985) Chronic inflammation and autoimmune disease. In: free radicals in biology and medicine, clarendon press, 279Ioannides C (2000) xenobiotic metabolism and bioactivation by cytochromes P450. In: Wiseman H, Goldfarb P, Ridgway T, Wiseman A (eds) Biomolecular free radical toxicity: causes and prevention. Chichester, John Wiley and Sons Limited, p. 103Google Scholar
- Halliwell B, Gutteridge JMC (1999) Free radicals in biology and medicine, 3rd edn. Oxford University Press, OxfordGoogle Scholar
- Kumar PP, Kumaravel S, et al. (2010) Screening of antioxidant activity, total phenolics and GC-MS study of Vitex negundo. Afr J Biochem Res 4:191–195Google Scholar
- Lad H, Dixit D, et al. (2015) Antioxidant and antiinflammatory effects of Vitex negundo on Freund’s complete adjuvant induced arthritis. Int J Pharma Pharmaceutical Sci 7:81–85Google Scholar
- Lakshmanashetty RH, Nagaraj VB, et al. (2010) In vitro antioxidant activity of Vitex negundo L. Leaf Extracts Chiang Mai J Sci 37(3):489–497Google Scholar
- Mehta AB, Hoffbrand AV (1999) Haematological aspects of systemic disease. In: Hoffbrand AV, Catovsky D, Tuddenham EGD (eds) Postgraduate Haematology; Chapt 59, 5th edn. Blackwell Publishing Ltd, HobokenGoogle Scholar
- Panday GS, Chunekar KC (1998) Bhav prakash nighantu Varanasi: Chaukhambha Bharati Academy p. 344–345Google Scholar
- Sabnis M (2006) Chemistry and pharmacology of ayurvedic medicinal plants, 1st edn. Varanasi: Chaukambha Amarbharati Prakashan; p. 363–366Google Scholar
- Singh H, Dixit A, et al. (2015) Comparative evaluation of total phenolic content, total flavonoid content and DPPH free radical scavenging activity of different plant parts of Vitex negundo. Int J Pharm Pharm Sci 7:144–147Google Scholar
- Tandon VR (2005) Medicinal uses and biological activities of Vitex negundo. Nat Pro Rad 4:162–165Google Scholar
- Telang RS, Chatterjee S, et al. (1999) Studies on analgesic and anti-inflammatory activities of Vitex negundo Linn. Ind J Pharmacol 31:363–366Google Scholar
- Zargar M, Azizah AH, et al. (2011) Bioactive compounds and antioxidant activity of different extracts from Vitex negundo leaf. J Med Plants Res 5:2525–2532Google Scholar