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Inhibitory activity of the protein carbonylation and hepatoprotective effect of the ethanol-soluble extract of Caesalpinia coriaria Jacq

Abstract

Free radical oxygen species cause protein carbonylation, an irreversible oxidative damage associated with diseases such as liver injury, diabetes mellitus, arthritis, cancer and Alzheimer. To determine the inhibitory activity of the protein carbonylation and the hepatoprotective effect of ethanol extract of the fruits of Caesalpinia coriaria Jacq (Fabaceae). Scavenging activity of 2,2-diphenyl-1- picrylhydrazyl radical (DPPH) and the radical cation 2,2’-azino-bis(3-ethylbenzothiazoline-sulfonic acid) (ABTS•+) of the extract of C. coriaria at 500 mg / L, was evaluated in a preliminary test. Inhibitory activity of the protein carbonylation isolated from mouse liver using Western methodology with dinitrophenylhydrazine (DNPH) probes was evaluated. The hepatoprotective activity of the extract was evaluated in vivo in Wistar rats using carbon tetrachloride (CCl4) as toxic orally. The degree of hepatoprotection was determined by measuring the serum liver enzymes, aspartate aminotransferase (AST) and alanine aminotransferase (ALT) and by histopathological analysis of rat liver. The rate of capture of the radicals DPPH and ABTS•+ by the extract of C. coriaria was higher than 90 % in both cases. The extract also showed a better inhibitory effect on the carbonylation of the proteins than butyl hydroxytoluene (BHT) used as positive control. The extract of C. coriaria produced a significant reduction of AST concentration (from 1667,67 ± 394,27 to 718,00 ± 24,85 U/L ) and ALT (from 967,67 ± 118,30 to 625.67 ± 60,98 U/L), indicating liver cell damage attenuation. The extract of C. coriaria produced an important antioxidant activity and moderate hepatoprotective effect against CCl4-induced toxicity.

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References

  • Adisakwattana S, Thilavech T, Chusak Ch (2014) Mesona Chinensis Benth extract prevents AGE formation and protein oxidation against fructose-induced protein glycation in vitro. BMC Complementary and Alternative Medicine [Online]. Available at:http://www.biomedcentral.com/1472-6882/14/130Accessed. 12 May 2015

  • Anandhi D, Revathi K (2013) Phytochemical analysis of Caesalpinia coriaria (Jacq) wild. Int J Biosci Res 2(1):1–7

    Google Scholar 

  • Arts IC, Hollman PC, Feskens EJ (2011) Catechin intake might explain the inverse relation between tea consumption and ischemic heart disease: the Zutphen elderly study. Am J Clin Nutr 74:227–232

    Google Scholar 

  • Bachi A, Dalle-Done I, Scaloni A (2013) Redox proteomics: chemical principles, methodological approaches and biological/biomedical promises. Chem Rev 113:596–698

    CAS  Article  PubMed  Google Scholar 

  • Berlett BS, Stadtman ER (1997) Protein oxidation in aging, disease, and oxidative stress. J Biol Chem 272(33):20313–20316

    CAS  Article  PubMed  Google Scholar 

  • Blokhina O, Virolainen E, Fagerstedt KV (2003) Antioxidants, oxidative damage and oxygen deprivation stress: a review. Ann Bot 91:179–194

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  • Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

    CAS  Article  PubMed  Google Scholar 

  • Chang YC, Chuang LM (2010) The role of oxidative stress in the pathogenesis of type 2 diabetes: from molecular mechanism to clinical implication. Am J Transl Res 2(3):316–331

    CAS  PubMed  PubMed Central  Google Scholar 

  • Codreanu SG, Liebler DC (2015) Novel approaches to identify protein adducts produced by lipid peroxidation. Free Radic Res 1–7

  • Curtis JM, Hahn WS, Long EK et al (2012) Protein carbonylation and metabolic control systems. Trends Endocrinol Metab 23(8):399–406

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  • Dalle-Donne I, Giustarini D, Colombo R et al (2003) Protein carbonylation in human diseases. Trends Mol Med 9(4):169–176

    CAS  Article  PubMed  Google Scholar 

  • Dewanto V, Wu X, Adom KK et al (2002) Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity. J Agric Food Chem 50:3010–3014

    CAS  Article  PubMed  Google Scholar 

  • Di Domenico F, Coccia R, Butterfield DA et al (2011) Circulating biomarkers of protein oxidation for Alzheimer disease: expectations within limits. Biochim Biophys Acta 1814:1785–1795

    Article  PubMed  Google Scholar 

  • Estévez M (2011) Protein carbonyls in meat systems: a review. Meat Sci 89:259–279

    Article  PubMed  Google Scholar 

  • Freeman BA, Crapo JD (1982) Biology of disease: free radicals and tissue injury. Lab Investig 47:412–426

    CAS  PubMed  Google Scholar 

  • Halliwell B (1994) Free radicals, antioxidants, and human disease: curiosity, cause, or consequence? Lancet 344(8924):721–724

    CAS  Article  PubMed  Google Scholar 

  • Hense HW, Stendera M, Borsc W et al (1993) Lack of an association between serum vitamin E and myocardial infarction in a population with high vitamin E levels. Atherosclerosis 103(1):21–28

    CAS  Article  PubMed  Google Scholar 

  • Hertog MGL, Feskens EJM, Hollman PCH et al (1993) Dietary antioxidant flavonoids and risk of coronary heart disease: the Zutphen elderly study. Lancet 342:1007–1011

    CAS  Article  PubMed  Google Scholar 

  • Hopps E, Noto D, Caimi G et al (2010) A novel component of the metabolic syndrome: the oxidative stress. Nutr Metab Cardiovasc Dis 20:72–77

    CAS  Article  PubMed  Google Scholar 

  • Lee C-P, Shih P-H, Hsu C-L et al (2007) Hepatoprotection of tea seed oil (Camellia oleifera Abel.) against CCl4-induced oxidative damage in rats. Food Chem Toxicol 45:888–895

    CAS  Article  PubMed  Google Scholar 

  • Lokeswari N, Sujatha P (2011) Isolation of tannins from Caesalpinia coriaria and effect of physical parameters. Int Res J Pharm 2(2):146–152

    Google Scholar 

  • Lushchak VI (2014) Free radicals, reactive oxygen species, oxidative stress and its 5 classification. Chem Biol Interact. doi:10.1016/j.cbi.2014.10.016, Article in press

    PubMed  Google Scholar 

  • Madhu Kiran P, Vijaya Raju A, Ganga Rao B (2012). Investigation of hepatoprotective activity of Cyathea gigantea (Wall. ex. Hook.) leaves against paracetamol-induced hepatotoxicity in rats. Asian Pac J Trop Biomed 352–356

  • Markesbery WR (1997) Oxidative stress hypothesis in Alzheimer’s disease. Free Radic Biol Med 23:134–147

    CAS  Article  PubMed  Google Scholar 

  • Mendez D, Linares M, Diez A et al (2011) Stress response and cytoskeletal proteins involved in erythrocyte membrane remodelling upon Plasmodium falciparum invasion are differentially carbonylated in G6PD A deficiency. Free Radic Biol Med 50(10):1305–1313

    CAS  Article  PubMed  Google Scholar 

  • Møller IM, Rogowska-Wrzesinska A, Rao RSP (2011) Protein carbonylation and metal-catalyzed protein oxidation in a cellular perspective. J Proteomics 74:2228–2242

    Article  PubMed  Google Scholar 

  • Navarro VJ, Senior JR (2006) Drug-related hepatotoxicity. N Engl J Med 354(7):731–739

    CAS  Article  PubMed  Google Scholar 

  • Niki E (2009) Lipid peroxidation: physiological levels and dual biological effects. Free Radic Biol Med 47:469–484

    CAS  Article  PubMed  Google Scholar 

  • Orhan IE, Sener B, Musharraf SG (2012) Antioxidant and hepatoprotective activity appraisal of four selected Fumaria species and their total phenol and flavonoid quantities. Exp Toxicol Pathol 64:205–209

    CAS  Article  PubMed  Google Scholar 

  • Ravikumar S, Gnanadesigan M (2012) Hepatoprotective and antioxidant properties of rhizophora mucronata mangrove plant in CCl4 intoxicated rats. J Exp Clin Med 4(1):66–72

    Article  Google Scholar 

  • Re R, Pellegrini N, Proteggente A et al (1999) Antioxidant activity applying an improved ABTS radical cation decollorization assay. Free Radic Biol Med 26:1231–1237

    CAS  Article  PubMed  Google Scholar 

  • Seki S, Kitada T, Sakaguhi H et al (2003) Pathological significance of oxidative cellular damage in human alcoholic liver disease. Histopathology 42:365–371

    CAS  Article  PubMed  Google Scholar 

  • Shaker E, Mahmoud H, Mnaa S (2010) Silymarin, the antioxidant component and Silybum marianum extracts prevent liver damage. Food Chem Toxicol 48:803–806

    CAS  Article  PubMed  Google Scholar 

  • Shukla S, Mehta A, John J et al (2009) Antioxidant activity and total phenolic content of ethanolic extract of Caesalpinia bonducella seeds. Food Chem Toxicol 47:1848–1851

    CAS  Article  PubMed  Google Scholar 

  • Sies H (1997) Oxidative stress: oxidants and antioxidants. Exp Physiol 82:291–295

    CAS  Article  PubMed  Google Scholar 

  • Silva B, Andrade P, Valentao P et al (2004) Quince (Cydonia oblonga Miller) fruit (pulp, peel, and seed) and jam: antioxidant activity. J Agric Food Chem 52:4705–4712

    CAS  Article  PubMed  Google Scholar 

  • Sivasankari K, Janaky S, Sekar T (2011) Antioxidant status of leaves of Caesalpinia bonduc. Int J Pharm Appl 2(4):262–266

    Google Scholar 

  • Vargas F, Rivas C, Nursama A et al (2007) Reacciones de radicales libres con relevancia biológica en la teoría del envejecimiento. Av Quím 2(2):3–15, Universidad de los Andes Mérida Venezuela

    CAS  Google Scholar 

  • Voulgaridoua G-P, Anestopoulosa I, Francob R et al (2011) DNA damage induced by endogenous aldehydes: current state of knowledge. Mutat Res 711:13–27

    Article  Google Scholar 

  • Yochum L, Kushi LH, Meyer K et al (1999) Dietary flavonoid intake and risk of cardiovascular disease in postmenopausal women. Am J Epidemiol 149:943–949

    CAS  Article  PubMed  Google Scholar 

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Acknowledgments

The authors thank to the University of Cartagena for funding this study; we also thank to the University Hospital of the Caribbean and to the Clinical Laboratory Eduardo Fernandez, for technical and scientific support. We also thank Mr. Moises Carrascal Medina and Miss Adriana Tinoco for collecting the plant material used in this study.

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Correspondence to F. Díaz Castillo.

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All experiments were performed at the University of Cartagena and the Laboratory of Pathology of the University Hospital of Caribbean, according to the guidelines of the International Council for Laboratory Animal and were approved by the ethical committee of the University Hospital of Caribbean, Cartagena, Colombia (Act No. 04, approval date 14 may 2012).

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The authors declare no conflict of Interest.

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Pájaro González, Y., Méndez Cuadro, D., Fernández Daza, E. et al. Inhibitory activity of the protein carbonylation and hepatoprotective effect of the ethanol-soluble extract of Caesalpinia coriaria Jacq. Orient Pharm Exp Med 16, 225–232 (2016). https://doi.org/10.1007/s13596-016-0228-8

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  • DOI: https://doi.org/10.1007/s13596-016-0228-8

Keywords

  • Hepatoprotective activity
  • Antioxidants
  • Western blot
  • Proteins carbonylation
  • Liver enzymes
  • Aspartate aminotransferase
  • Alanine aminotransferase