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Inhibitory activity of the protein carbonylation and hepatoprotective effect of the ethanol-soluble extract of Caesalpinia coriaria Jacq

Oriental Pharmacy and Experimental Medicine volume 16, pages 225–232 (2016)Cite this article

Abstract

Free radical oxygen species cause protein carbonylation, an irreversible oxidative damage associated with diseases such as liver injury, diabetes mellitus, arthritis, cancer and Alzheimer. To determine the inhibitory activity of the protein carbonylation and the hepatoprotective effect of ethanol extract of the fruits of Caesalpinia coriaria Jacq (Fabaceae). Scavenging activity of 2,2-diphenyl-1- picrylhydrazyl radical (DPPH•) and the radical cation 2,2’-azino-bis(3-ethylbenzothiazoline-sulfonic acid) (ABTS•+) of the extract of C. coriaria at 500 mg / L, was evaluated in a preliminary test. Inhibitory activity of the protein carbonylation isolated from mouse liver using Western methodology with dinitrophenylhydrazine (DNPH) probes was evaluated. The hepatoprotective activity of the extract was evaluated in vivo in Wistar rats using carbon tetrachloride (CCl4) as toxic orally. The degree of hepatoprotection was determined by measuring the serum liver enzymes, aspartate aminotransferase (AST) and alanine aminotransferase (ALT) and by histopathological analysis of rat liver. The rate of capture of the radicals DPPH• and ABTS•+ by the extract of C. coriaria was higher than 90 % in both cases. The extract also showed a better inhibitory effect on the carbonylation of the proteins than butyl hydroxytoluene (BHT) used as positive control. The extract of C. coriaria produced a significant reduction of AST concentration (from 1667,67 ± 394,27 to 718,00 ± 24,85 U/L ) and ALT (from 967,67 ± 118,30 to 625.67 ± 60,98 U/L), indicating liver cell damage attenuation. The extract of C. coriaria produced an important antioxidant activity and moderate hepatoprotective effect against CCl4-induced toxicity.

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Acknowledgments

The authors thank to the University of Cartagena for funding this study; we also thank to the University Hospital of the Caribbean and to the Clinical Laboratory Eduardo Fernandez, for technical and scientific support. We also thank Mr. Moises Carrascal Medina and Miss Adriana Tinoco for collecting the plant material used in this study.

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Authors and Affiliations

  1. Research Group in Welfare Pharmacy and Pharmacology, Atlántic University, Barranquilla, Colombia

    Y. Pájaro González

  2. Analytical Chemistry and Biomedicine Group, University of Cartagena, Cartagena, Colombia

    D. Méndez Cuadro

  3. Faculty of Medicine, University of Cartagena, Cartagena, Colombia

    E. Fernández Daza

  4. Biological Evaluation of Promising Substances Group, University of Cartagena, Cartagena, Colombia

    L. A. Franco Ospina

  5. Laboratory of Pathology of the University Hospital of Caribbean, University of Cartagena, Cartagena, Colombia

    C. Redondo Bermúdez

  6. Laboratory of Phytochemical and Pharmacological Researchs, University of Cartagena, Cartagena, Colombia

    F. Díaz Castillo

  7. Faculty of Pharmaceutical Sciences, University of Cartagena, Cartagena, Colombia

    F. Díaz Castillo

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  1. Y. Pájaro González
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  2. D. Méndez Cuadro
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  3. E. Fernández Daza
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  4. L. A. Franco Ospina
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  5. C. Redondo Bermúdez
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  6. F. Díaz Castillo
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Corresponding author

Correspondence to F. Díaz Castillo.

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Ethical statement

All experiments were performed at the University of Cartagena and the Laboratory of Pathology of the University Hospital of Caribbean, according to the guidelines of the International Council for Laboratory Animal and were approved by the ethical committee of the University Hospital of Caribbean, Cartagena, Colombia (Act No. 04, approval date 14 may 2012).

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Pájaro González, Y., Méndez Cuadro, D., Fernández Daza, E. et al. Inhibitory activity of the protein carbonylation and hepatoprotective effect of the ethanol-soluble extract of Caesalpinia coriaria Jacq. Orient Pharm Exp Med 16, 225–232 (2016). https://doi.org/10.1007/s13596-016-0228-8

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Keywords

  • Hepatoprotective activity
  • Antioxidants
  • Western blot
  • Proteins carbonylation
  • Liver enzymes
  • Aspartate aminotransferase
  • Alanine aminotransferase