Oriental Pharmacy and Experimental Medicine

, Volume 13, Issue 2, pp 127–131 | Cite as

Rhododendron arboreum (Ericaceae): immunomodulatory and related toxicity studies

  • Pankaj Kumar Sonar
  • Ranjit Singh
  • Anju Verma
  • Shailendra K. Saraf
Research Article

Abstract

In the present study, an attempt was made to evaluate the extract of the leaves of Rhododendron arboreum for immunomodulatory activity and related hepatotoxicity. Alcoholic extract of the leaves of R. arboreum was prepared by hot percolation method. Levamisole was used as the standard drug. The parameters like, humoral immune response, cell mediated immune response and total leukocyte counts (TLC) were assessed in the antigenically challenged mice with sheep RBC (SRBC). Liver function tests like total bilirubin, SGPT and SGOT were also evaluated. The, orally administered alcoholic extract of the leaves showed a significant suppression of the immune responses, in a dose dependent manner. The extract at the maximum dose (100 mg/kg) was found to possess higher immunosuppressant effect in comparison with control and levamisole (p < 0.001). No significant difference was observed in the weights of liver and spleen of extract-treated mice in comparison with levamisole and vehicle treated mice. Liver function tests of extract-treated mice were also statistically insignificant in comparison with levamisole and vehicle-treated mice. Thus, it can be concluded that the alcoholic extract of R. arboreum is an effective and safe immunosuppressive agent.

Keywords

R. arboreum Haemagglutination DTH Phagocytosis SRBC 

References

  1. Bafna AR, Mishra SH (2006) Immunostimulatory effect of methanol extract of Curculigo orchioides on immunosuppressed mice. J Ethnopharmacol 104(1–2):1–4PubMedCrossRefGoogle Scholar
  2. Bergmeyer HU, Scheibe P, Wahlefeld AW (1978) Optimization of methods for aspartate aminotransferase and alanine aminotransferase. Clin Chem 24(1):58–73PubMedGoogle Scholar
  3. Bergmeyer HU, Hoder M, Rej R (1986) Approved recommendation on International Federation of Clinical Chemistry methods for the measurement of catalytic concentration of enzymes. J Clin Chem Clin Biochem 24(7):497–510PubMedGoogle Scholar
  4. Bhandary MR, Kawabata J (2008) Antidiabitic activity of laligurans (Rhododendron arboreum Sm.) flower. J Food Sci Technol Nepal 4:61–63Google Scholar
  5. Dhar ML, Dhar MM, Dhawan BN, Ray C (1968) Screening of Indian medicinal plants for biological activity. Ind J Exp Biol 6:232–247Google Scholar
  6. Evans WC (2009) Carbohydrate. In: Evans WC (ed) Trease and Evans pharmacognosy, 16th edn. Saunders Elsevier Limited, New York, pp 196–197Google Scholar
  7. Fakeye OT, Pal A, Bawankule DU, Khanuja SPS (2008) Immunomodulatory effect of extracts of Hibiscus sabdariffa L. (Family Malvaceae) in a mouse model. Phytother Res 22(5):664–668PubMedCrossRefGoogle Scholar
  8. Jendraaaik J, Grof P (1938) Vereinfachte photometrische Methoden zur Beetimmung des Blutbilirubin. Biochem Z 297:81–89Google Scholar
  9. Jinyvargese K, Kapse ST, Kulkarni SR (2005) Immunostimulant activity of Adhatoda vasica, Lawsonia inermis and Alkanna tinctoria. Indian Drugs 42(6):345–352Google Scholar
  10. Joharapurkar AA, Wanjari MM, Dixit PV, Zambad SP, Umathe SN (2004) Pyrogallol: a novel tool for screening immunomodulators. Indian J Pharmacol 36(6):355–359Google Scholar
  11. Koko WS, Mesaik MA, Yousaf S, Galal M, Choudhary MI (2008) In vitro immunomodulating properties of selected Sudanese medicinal plants. J Ethnopharmacol 118(1):26–34PubMedCrossRefGoogle Scholar
  12. Larrey D (2008) Hépatotoxicité des immunosuppresseurs. conduite diagnostique hepatotoxicity of immunosuppressants. Diagnostic approach. Gastroen Clin Biol 32(5 Pt 2):S194–S204CrossRefGoogle Scholar
  13. Midlekoop TB, Lababie RP (1983) Evaluation of Asoka aristha, an indigenous medicine in Sri Lanka. J Ethnopharmacol 8(3):13–320Google Scholar
  14. Prakash T, Fadadu SD, Sharma UR, Surendra V, Goli D, Stamina P et al (2008) Hepatoprotective activity of leaves of Rhododendron arboreum in CCl4 induced hepatotoxicity in rats. J Med Plants Res 2(11):315–320Google Scholar
  15. Sharma UR, Surendra V, Jha SK, Nitesh SC, Prakash T, Goli D (2009) Evaluation of anti-inflammatory activity of Rhododendron arboreum herb extract on experimental animal. Arch Pharm Sci Res 1:58–61Google Scholar
  16. Sonar PK, Khan S, Singh R, Saraf SK (2012a) Isolation, characterization and activity of the flowers of Rhododendron arboreum (Ericaceae). E-J Chem 9(2):631–636CrossRefGoogle Scholar
  17. Sonar PK, Singh R, Bansal P, Balapure AK, Saraf SK (2012b) R. arboreum flower and leaf extracts: RP-HPTLC screening, isolation, characterization and biological activity. RASAYAN J Chem 5(2):165–172Google Scholar
  18. Toscano E, Cotta J, Robles M, Lucena MA, Andrade RJ (2010) Hepatotoxicity induced by new immunosuppressants. Gastroenterol Hepatol 33(1):54–65PubMedCrossRefGoogle Scholar
  19. Verma N, Singh AP, Amresh G, Sahu PK, Rao CV (2011) Protective effect of ethyl acetate fraction of Rhododendron arboreum flowers against carbon tetrachloride-induced hepatotoxicity in experimental models. Indian J Pharmacol 43(3):291–295PubMedCrossRefGoogle Scholar
  20. Vogel AI (2000) Reactions and characterization of selected classes of organic compounds. In: Vogel AI (ed) Elementry practical organic chemistry part 2: Quantitative organic analysis, 2nd edn. CBS Publishers and Distributors, India, pp 89–91Google Scholar

Copyright information

© Institute of Oriental Medicine, Kyung Hee University 2013

Authors and Affiliations

  • Pankaj Kumar Sonar
    • 1
    • 4
  • Ranjit Singh
    • 2
  • Anju Verma
    • 3
  • Shailendra K. Saraf
    • 1
  1. 1.Faculty of PharmacyBabu Banarasi Das Northern India Institute of TechnologyLucknowIndia
  2. 2.Government College of PharmacyShimlaIndia
  3. 3.Divison of PharmacognosySaroj Institute of Technology and ManagementLucknowIndia
  4. 4.Pharmaceutical Chemistry, Faculty of PharmacyBabu Banarasi Das Northern India Institute of TechnologyLucknowIndia

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