Abstract
Ginsenoside Rb1 has been determined to exert diverse neuromodulatory effects including a stimulatory effect on synaptic formation. Synaptophysin and microtubule-associated protein 2 (MAP2) are synaptic proteins which play vital roles in neurotransmission regulation and synaptic structural stability. The present data revealed that ginsenoside Rb1 enhanced mRNA expression of synaptic markers, synaptophysin and MAP2, in primary cultured rat hippocampal neurons. Our results newly add synaptophysin and MAP2 to the list of genes of which mRNA expression is modulated by ginsenosides. Our finding contributes further understanding of medical usefulness of ginsenoside Rb1 targeting hippocampal network alteration which is commonly observed in aging and neurodegenerative disorders.
References
Benishin CG, Lee R, Wang LC, Liu HJ (1991) Effects of ginsenoside Rb1 on central cholinergic metabolism. Pharmacology 42:223–229
Dehmelt L, Halpain S (2005) The MAP2/Tau family of microtubule-associated proteins. Genome Biol 6:204
Kim S, Ahn K, Oh TH, Nah SY, Rhim H (2002) Inhibitory effect of ginsenosides on NMDA receptor-mediated signals in rat hippocampal neurons. Biochem Biophys Res Commun 296:247–254
Kim S, Kim T, Ahn K, Park WK, Nah SY, Rhim H (2004) Ginsenoside Rg3 antagonizes NMDA receptors through a glycine modulatory site in rat cultured hippocampal neurons. Biochem Biophys Res Commun 323:416–424
Kim SO, You JM, Yun SJ, Son MS, Nam KN, Hong JW, Kim SY, Choi SY, Lee EH (2010) Ginsenoside rb1 and rg3 attenuate gluocorticoid-induced neurotoxicity. Cell Mol Neurobiol 30:857–862
Kim YC, Kim SR, Markelonis GJ, Oh TH (1998) Ginsenosides Rb1 and Rg3 protect cultured rat cortical cells from glutamate-induced neurodegeneration. J Neurosci Res 53:426–432
Kim YG, Lee YI (2012) Differential expressions of synaptogenic markers between primary cultured cortical and hippocamapl neurons. Exp Neurobiol 21:61–67
Lian XY, Zhang Z, Stringer JL (2006) Anticonvulsant and neuroprotective effects of ginsenosides in rats. Epilepsy Res 70:244–256
Lim JH, Wen TC, Matsuda S, Tanaka J, Maeda N, Peng H, Aburaya J, Ishihara K, Sakanaka M (1997) Protection of ischemic hippocampal neurons by ginsenoside Rb1, a main ingredient of ginseng root. Neurosci Res 28:191–200
Liu L, Gia TH, Wu H, Lee MR, Gu L, Wang C, Yun BS, Wang Q, Ye S, Sung CK (2011) Ginsenoside Rb1 improves spatial learning and memory by regulation of cell genesis in the hippocampal subregions of rats. Brain Res 1382:147–154
Mook-Jung I, Hong HS, Boo JH, Lee KH, Yun SH, Cheong MY, Huh K, Jung MW (2001) Ginsenoside Rb1 and Rg1 improve spatial learning and increase hippocampal synaptophysin level in mice. J Neurosci Res 63:509–515
Neves G, Cooke SF, Bliss TV (2008) Synaptic plasticity, memory and the hippocampus: a neural network approach to causality. Nat Rev Neurosci 9:65–75
Park JK, Namgung U, Lee CJ, Park JO, Jin SH, Kwon OB, Ko SR, Kim SW, Kang EJ, Ko JH, Lee SM, Kim DH, Won MH (2005) Calcium-independent CaMKII activity is involved in ginsenoside Rb1-mediated neuronal recovery after hypoxic damage. Life Sci 76:1013–1025
Pfaffl MW, Horgan GW, Dempfle L (2002) Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR. Nucleic Acids Res 30:e36
Radad K, Gille G, Moldzio R, Saito H, Rausch WD (2004a) Ginsenosides Rb1 and Rg1 effects on mesencephalic dopaminergic cells stressed with glutamate. Brain Res 1021:41–53
Radad K, Gille G, Moldzio R, Saito H, Ishige K, Rausch WD (2004b) Ginsenosides Rb1 and Rg1 effects on survival and neurite growth of MPP + -affected mesencephalic dopaminergic cells. J Neural Transm 111:37–45
Rudakewich M, Ba F, Benishin CG (2001) Neurotrophic and neuroprotective actions of ginsenosides Rb(1) and Rg(1). Planta Med 67:533–537
Sakanaka M, Zhu P, Zhang B, Wen TC, Cao F, Ma YJ, Samukawa K, Mitsuda N, Tanaka J, Kuramoto M, Uno H, Hata R (2007) Intravenous infusion of dihydroginsenoside Rb1 prevents compressive spinal cord injury and ischemic brain damage through upregulation of VEGF and Bcl-xL. J Neurotrauma 24:1037–1054
Salim KN, McEwen BS, Chao HM (1997) Ginsenoside Rb1 regulates ChAT, NGF, and trkA mRNA expression in the rat brain. Brain Res Mol Brain Res 47:177–182
Tohda C, Matsumoto N, Zou K, Meselhy MR, Komatsu K (2004) Aβ(25-25)-induced memory impairment, axonal atrophy, and synaptic loss are ameliorated by M1, a metabolite of protopanaxadiol-type saponins. Neuropsychopharmacology 29:860–868
Ubhi K, Masliah E (2012) Alzheimer’s disease: recent advances and future perspectives. J Alzheimers Dis. doi:10.3233/JAD-2012-129028
Wang L, Kisaalita WS (2011) Administration of BDNF/ginsenosides combination enhanced synaptic development in human neural stem cells. J Neurosci Methods 194:274–282
Yuan QL, Yang CX, Xu P, Gao XQ, Deng L, Chen P, Sun ZL, Chen QY (2007) Neuroprotective effects of ginsenoside Rb1 on transient cerebral ischemia in rats. Brain Res 1167:1–12
Zhu J, Jiang Y, Wu L, Lu T, Xu G, Liu X (2012) Suppression of local inflammation contributes to the neuroprotective effects of ginsenoside Rb1 in rats with cerebral ischemia. Neuroscience 202:342–351
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This study was supported by a grant (20081531) from Kyung Hee University in the program year 2009.
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Nam, K.N., Jung, WS., Park, JH. et al. Ginsenoside Rb1 increases synaptophysin and microtubule-associated protein 2 mRNA expression in primary cultures of rat hippocampal neurons. Orient Pharm Exp Med 13, 231–234 (2013). https://doi.org/10.1007/s13596-012-0101-3
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DOI: https://doi.org/10.1007/s13596-012-0101-3