Anti-histamine release and anti-inflammatory activities of aqueous extracts of citrus fruits peels
- 642 Downloads
In this study, aqueous extracts of peels of best known citrus fruits namely grapefruit (Citrus paradisi), lemon (C. limon), lime (C. aurantifolia) and orange (C. sinensis) were used. Depending on polyphenols content, the extracts were graded as orange > lemon > lime > grapefruit. Effects of the extracts on the release of histamine from rat peritoneal exudate cells (PECs) was measured to know anti-allergic activity. All extracts inhibited the release of histamine from rat PECs induced by the calcium ionophore A23187. Heating of the extracts at 100 °C for 10 min enhanced inhibition of histamine release. Effect of the oral administration of aqueous extract of grapefruit peel on the effusion of Chicago sky blue dye into the intraperitoneal cavity of mice was measured to know the anti-inflammatory activity. The extract potentially suppressed inflammation in the mice cavity as like indometacin, an anti-inflammatory drug. Therefore, the extracts of citrus peels are useful for developing anti-allergic and anti-inflammatory foods and supplements.
KeywordsAnti-allergy Anti-histamine release Anti-inflammation Citrus peel Polyphenols
The authors thank Dr. Mikako Takasugi in Department of Applied Chemistry and Biochemistry, Faculty of Engineering, Kyushu Sangyo University for guidance with measuring the release of histamine from rat PECs.
- Ho SC, Lin CC (2008) Investigation of heat treating conditions for enhancing the anti-inflammatory activity of citrus fruit (Citrus reticulata) peels. J Agric Food Chemistry 56:7976–7982Google Scholar
- Hossain SJ, Aoshima H, El-Sayed M, Ahmed F (2009) Antioxidative and anti-histamine-release activities of Excoecaria agallocha L. Pharmacologyonline 2:927–936Google Scholar
- Hossain SJ, Basar MH, Rokeya B, Arif KMT, Sultana MS, Rahman MH (2012) Evaluation of antioxidant, antidiabetic and antibacterial activities of the fruit of Sonneratia apetala (Buch.-Ham.). Orient Pharm Exp Med. doi: 10.1007/s13596-012-0064-4
- Mubassara S, Takasugi M, Iga R, Hossain SJ, Aoshima H (2011) Inhibition of the histamine and leukotriene B4 release from rat peritoneal exudates cells by six Bangladeshi plants. Pharmacologyonline 2:76–85Google Scholar
- Murakami A, Nakamura Y, Torikai K, Tanaka T, Koshiba T, Koshimizu K, Kuwahara S, Takahashi Y, Ogawa K, Yano M, Tokuda H, Nishino H, Mimaki Y, Sashida Y, Kitanaka S, Ohigashi H (2000) Inhibitory effect of citrus nobiletin on phorbol ester-induced skin inflammation, oxidative stress, and tumor promotion in mice. Cancer Res 60:5059–5066PubMedGoogle Scholar
- Takasugi M, Shimada K, Yamada K (2002) Studies on inhibitory effect of soy isoflavones on release of allergy-related mediators from rat peritoneal exudates cells. Soy Protein Res 5:99–102Google Scholar
- Younginger JW (1992) Anaphylaxis. Ann Allergy 69:87–96Google Scholar