Evaluation of phytochemical and antimicrobial properties of Commelina diffusa Burm. f.
- 359 Downloads
The results revealed that different fractions of Commelina diffusa Burm. f. produced significant (p < 0.001, p < 0.01, p < 0.05, p < 0.02 and p < 0.5) inhibitory activity against the test bacteria and fungi. Methanolic fraction produced the highest zone of inhibition against the test bacteria (11 to 19 mm) which was followed by diethyl etheric extract (3 to 9 mm) and petroleum etheric extract (1 to 2 mm). Again, the methanolic extract showed the highest activity (the lowest MICs; 15.62 to 62.5 μg/μl) against seven species among the 11. In case of diethyl etheric and petroleum etheric fractions MICs were found to be (15.62 to 125 μg/μl) and (31.25 to 500 μg/μl), respectively. The diethyl etheric fraction showed the highest zone of inhibition against the fungi (15 to 19 mm) which was followed by methanolic extract (12 to 19 mm) and petroleum etheric extract (1 to 9 mm). Against the fungi the diethyl etheric extract showed the highest activity (i.e. the lowest MICs; 15.62 to 31.25 μg/μl). In case of methanolic and petroleum etheric fractions minimum inhibitory concentrations were found to be (15.62 to 125 μg/μl) and (31.25 to 250 μg/μl), respectively.
KeywordsCommelina diffusa Burm. f. Commelinaceae Antibacterial Antifungal Phytochemical
Sincere thanks for the cooperation to the Head, Department of Microbiology, University of Chittagong, Bangladesh for providing the test organisms to carry out the research work and Mr. Mhammed Mohiuddin for taxonomic identification of the sample.
- Bauer AW, Kirby WMM, Sherris JC, Tuck M (1996) Antibiotics susceptibility testing by a standardized single disc method. Am J Clin Pathol 45:493–496Google Scholar
- David BLAc (1998) Medicine at your feet: healing plants of the Hawaiian Kingdom Commelina diffusa (Honohono). Publishing web: www.medicineatyourfeet.com. Accessed 1998
- Islam MA, Alam MM, Choudhury ME, Kobayashi N, Ahmed MU (2008) Determination of minimum inhibitory concentration (MIC) of cloxacillin for selected isolates of methicillin-resistant Staphylococcus aureus (mrsa) with their antibiogram. Bangladesh J Vet Med 6(1):121–126Google Scholar
- Mahesh B, Satish S (2008) Antimicrobial activity of some important medicinal plant against plant and human pathogens. World J Agric Sci 4(S):839–843Google Scholar
- Pathmanathan MK, Uthayarasa K, Jeyadevan JP, Jeyaseelan EC (2010) In vitro antibacterial activity and phytochemical analysis of some selected medicinal plants. Int J Pharm Biol Arch 1(3):291–299Google Scholar
- Rahman AU, Choudhary MI, Thomson WJ (2005) Bioassay techniques for drug development. Taylor & Francis e-library, LondonGoogle Scholar
- Vogel AI (2000) Column and thin layer chromatography. In: Mendham J, Denney RC (eds) Text book of quantitative chemical analysis, 6th edn. Prentice Hall, New Jersey, pp 279–288Google Scholar
- Wahab OM, Ayodele AE, Moody JO (2010) TLC phytochemical screening in some Nigerian Loranthaceae. J Pharmacogn Phytother 2(5):64–70Google Scholar