Photosynthesis and growth responses of Fraxinus mandshurica Rupr. seedlings to a gradient of simulated nitrogen deposition
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During an N-deposition simulation experiment, we showed that low to medium addition of N had beneficial effects on growth and photosynthetic rates of Fraxinus mandshurica Rupr. seedlings, while beyond a threshold of 80 kg N ha −1 year −1 , performance plateaued and even declined at higher immissions.
Temperate forests are shifting from naturally N-limited toward N-saturated status with increasing N deposition. Yet, our knowledge regarding how seedling growth and physiology respond to excessive N input in temperate tree species remains very limited.
The objective of this study was to examine growth and photosynthetic responses of F. mandshurica seedlings to a gradient of simulated N deposition.
We conducted a 4-year study to investigate growth and photosynthetic responses of F. mandshurica seedlings to a large gradient of simulated N deposition (0, 20, 40, 60, 80, 100, and 120 kg N ha−1 year−1). Biomass accumulation and allocation, photosynthetic gas exchange, expression, and activities of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) in leaves were determined during the fourth growing season. Soil biochemical properties were measured to link them to the alterations in growth and photosynthetic traits across the N addition gradient.
Seedling growth and photosynthesis were dependent upon the rates of N deposition. The maximum rate of carboxylation (V c,max) and the net photosynthetic rate under saturating light (A sat) reached a maximum under 60 kg N ha−1 year−1. By contrast, high-level N inputs (100 and 120 kg N ha−1 year−1) resulted in suboptimal values in biomass and photosynthetic activity. Nitrogen deposition also modulated the activity and expression of Rubisco in leaves with a maximum around 80–100 kg N ha−1 year−1. Redundancy analysis (RDA) showed that the changes of seedling growth and photosynthesis along the gradient of N deposition were mostly attributed to the variations of soil pH and total N content.
Our data suggest that the threshold of N deposition is about 80 kg N ha−1 year−1 for F. mandshurica seedlings in this region. Excessive N input decreased performance on the seedling growth and photosynthesis.
KeywordsFraxinus mandshurica Photosynthetic response Nitrogen deposition Rubisco Biomass production
We thank the staff from CBFERS for their assistance in collecting field data.
Some metadata are available at http://doi.org/10.5281/zenodo.939384
This work was funded by the National Natural Science Foundation of China (31722013, 31500222, 31670412) the key project of Ministry of Science and Technology of China (2016YFA0600803), the project QYZDJ-SSW-DQC027 and the Hundred Talents Program from the Chinese Academy of Sciences.
Compliance with ethical standards
The authors declare that they have no competing interests.
- Aber JD, Goodale CL, Ollinger SV, Smith ML, Magill AH, Martin ME, Hallett RA, Stoddard JL (2003) Is nitrogen deposition altering the nitrogen status of northeastern forests? Bio Science 53:375–389Google Scholar
- Bobbink R, Hicks K, Galloway J, Spranger T, Alkemade R, Ashmore M, Bustamante M, Cinderby S, Davidson E, Dentener F, Emmett B, Erisman JW, Fenn M, Gilliam F, Nordin A, Pardo L, De Vries W (2010) Global assessment of nitrogen deposition effects on terrestrial plant diversity: a synthesis. Ecol Appl 20(1):30–59. https://doi.org/10.1890/08-1140.1 CrossRefPubMedGoogle Scholar
- Boxman AW, Blanck K, Brandrud TE, Emmett BA, Gundersen P, Hogervorst RF, Kjonaas OJ, Persson H, Timmermann V (1998) Vegetation nd soil biota response to experimentally-changed nitrogen inputs in coniferous forest ecosystems of the NITREX project. For Ecol Manag 101(1-3):65–79. https://doi.org/10.1016/S0378-1127(97)00126-6 CrossRefGoogle Scholar
- Carroll JA, Caporn SJM, Johnson D, Morecroft MD, Lee JA (2003) The interactions between plant growth, vegetation structure and soil processes in semi–natural acidic and calcareous grasslands receiving long–term inputs of simulated pollutant nitrogen deposition. Environ Pollution 21:363–376CrossRefGoogle Scholar
- Ethier GJ, Livingston NJ, Harrison DL, Black TA, Moran JA (2006) Low stomatal and internal conductance to CO2 versus Rubisco deactivation as determinants of the photosynthetic decline of ageing evergreen leaves. Plant Cell Environ 29(12):2168–2184. https://doi.org/10.1111/j.1365-3040.2006.01590.x CrossRefPubMedGoogle Scholar
- Fenn ME, Poth MA, Aber JD, Baron JS, Bormann BT, Johnson DW, Lemly AD, McNulty SG, Ryan DF, Stottlemyer R (1998) Nitrogen excess in North American ecosystems: predisposing factors, ecosystem responses, and management strategies. Ecol Appl 8(3):706–733. https://doi.org/10.1890/1051-0761(1998)008[0706:NEINAE]2.0.CO;2 CrossRefGoogle Scholar
- Galloway JN, Aber JD, Erisman JW, Seitzinger SP, Howarth RW, Cowling EB, Cosby BJ (2003) The nitrogen cascade. Bioscience 53(4):341–356. https://doi.org/10.1641/0006-3568(2003)053[0341:TNC]2.0.CO;2 CrossRefGoogle Scholar
- Hyvönen R, Agren GI, Linder S, Persson T, Cotrufo MF, Ekblad A, Freeman M, Grelle A, Janssens IA, Jarvis PG, Kellomäki S, Lindroth A, Loustau D, Lundmark T, Norby RJ, Oren R, Pilegaard K, Ryan MG, Sigurdsson BD, Strömgren M, van Oijen M, Wallin G (2007) The likely impact of elevated (CO2), nitrogen deposition, increased temperature and management on carbon sequestration in temperate and boreal forest ecosystems: a literature review. New Phytol 173(3):463–480. https://doi.org/10.1111/j.1469-8137.2007.01967.x CrossRefPubMedGoogle Scholar
- Kinose Y, Azuchi F, Uehara Y, Kanomata T, Kobayashi A, Yamaguchi M, Izuta T (2014) Modeling of stomatal conductance to estimate stomatal ozone uptake by Fagus crenata, Quercus serrata, Quercus mongolica var. crispula and Betula platyphylla. Environ Pollut 194:235–245. https://doi.org/10.1016/j.envpol.2014.07.030 CrossRefPubMedGoogle Scholar
- Kinose Y, Fukamachi Y, Okabe S, Hiroshima H, Watanabe M, Izuta T (2017) Photosynthetic responses to ozone of upper and lower canopy leaves of Fagus crenata Blume seedlings grown under different soil nutrient conditions. Environ Pollut 223:213–222. https://doi.org/10.1016/j.envpol.2017.01.014 CrossRefPubMedGoogle Scholar
- Lambers H, Chapin FS, Pons TL (1998) Plant physiological ecology. Springer Verlag, New York 540, DOI: https://doi.org/10.1007/978-1-4757-2855-2
- Li D, Mo J, Fang Y, Cai X, Xue J, Xu G (2004) Effects of simulated nitrogen deposition on growth and photosynthesis of Schima superba, Castanopsis chinensis and Cryptocarya concinna seedlings. Acta Ecol Sin 24:876–882Google Scholar
- Nakaji T, Fukami M, Dokiya Y, Izuta T (2001) Effects of high nitrogen load on growth, photosynthesis and nutrient status of Cryptomeria japonica and Pinus densiflora seedlings. Tree Str Funct 15:453–461Google Scholar
- Stitt M, Schulze ED (1994) Does Rubisco control the rate of photosynthesis and plant growth? An exercise in molecular ecophysiology. Plant Cell Environ 17(5):465–487. https://doi.org/10.1111/j.1365-3040.1994.tb00144.x CrossRefGoogle Scholar
- Wang M, Shi S, Lin F, Hao Z, Jiang P, Dai G (2012) Effects of soil water and nitrogen on growth and photosynthetic response of Manchurian ash (Fraxinus mandshurica) seedlings in northeastern China. PLoS One 7(2):e30754. https://doi.org/10.1371/journal.pone.0030754 CrossRefPubMedPubMedCentralGoogle Scholar
- Warren CR, Dreyer E, Adams MA (2003) Photosynthesis-Rubisco relationships in foliage of Pinus sylvestris in response to nitrogen supply and the proposed role of Rubisco and amino acids as nitrogen stores. Trees 17:359–366Google Scholar
- Wortman E, Tomaszewski T, Waldner P, Schleppi P, Thimonier A, Eugster W, Buchmann N, Sievering H (2012) Atmospheric nitrogen deposition and canopy retention influences on photosynthetic performance at two high nitrogen deposition Swiss forests. Tellus B 64(1):17216. https://doi.org/10.3402/tellusb.v64i0.17216 CrossRefGoogle Scholar
- Zhang M, Guan DX, Han SJ, Wu J, Zhang J, Jin M, Dai G (2005) Climatic dynamics of broadleaved Korean pine forest in Changbai Mountain during the last 22 years. Chin J Ecol 24:1007–1012Google Scholar
- Zhou WM, Guo Y, Zhu BK, Wang XY, Zhou L, Yu DP, Dai L (2015) Seasonal variations of nitrogen flux and composition in a wet deposition forest ecosystem on Changbai Mountain. Acta Ecol Sin 35:158–164Google Scholar