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Safranal transference from ewe's milk to cheese and whey and antifungal properties of fortified whey

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Dairy Science & Technology

Abstract

Growth of undesirable moulds is one of the major causes of defects in modern cheese production, and it is necessary to discover natural antifungal substances that are non-toxic to humans. Saffron spice has been successfully used as a new ingredient in some Italian or Spanish cheeses, but transference of safranal (the main compound responsible for saffron aroma) during cheesemaking has not yet been studied. In this study, the distribution of safranal in cheese, whey and r equesón (Spanish whey cheese) and the antifungal potential of whey fractions against the mould Penicillium verrucosum were studied. To this end, a duplicate fabrication of cheeses was performed with raw ewe's milk with the addition of 40 μg safranal.kg−1 or control milk (no safranal addition). Safranal was more concentrated in cheese and requesón (78.91 and 76.95 μg.kg−1, respectively) than in whey and requesón whey (35.19 and 34.64 μg.kg−1, respectively). These liquid fractions showed good antifungal properties and inhibited mould with a safranal concentration of around 35 μg.kg−1, while a solution of 80 μg.kg−1 was required for a standard safranal solution to obtain a similar inhibition. These results reveal that whey has a synergistic effect with safranal in preventing cheese spoilage by moulds, which encourages making full use of this problematic by-product.

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References

  • Bakkali F, Averbeck S, Averbeck D, Idaomar M (2008) Biological effects of essential oils—a review. Food Chem Toxicol 46:446–475

    Article  CAS  Google Scholar 

  • Baratta MT, Dorman HJD, Deans SG, Figueiredo AC, Barroso JG (1998) Antimicrobial and antioxidant properties of some commercial essential oils. Flavour Fragance J 13:235–244

    Article  CAS  Google Scholar 

  • Belitz HD, Grosch W, Schieberle P (2009) Food contamination. In: Food chemistry, 4th edn. Springer, Berlin, pp. 472–475

  • Carpino S, Rapisarda T, Belvedere G, Licitra G (2008) Volatile fingerprint of Piacentinu cheese produced with different tools and type of saffron. Small Rum Res 79:16–21

    Article  Google Scholar 

  • Clément M, Tremblay J, Lange M, Thibodeau J, Belhumeur P (2008) Purification and identification of bovine cheese whey fatty acids exhibiting in vitro antifungal activity. J Dairy Sci 91:25–35

    Article  Google Scholar 

  • González-Chávez SA, Arévalo-Gallegos S, Rascón-Cruz Q (2009) Lactoferrin: structure, function and applications. Int J Antimicrobial Agents 33:301–308

    Article  Google Scholar 

  • Hayaloglu AA, Kirbag S (2007) Microbial quality and presence of moulds in Kuflu cheese. Int J Food Microbiol 115:376–380

    Article  CAS  Google Scholar 

  • Hernández-Ledesma B, Ramos M, Gómez-Ruiz JA (2011) Bioactive components of ovine and caprine cheese whey. Small Rum Res 101:196–204

    Article  Google Scholar 

  • Hosseinzadeh H, Noraei NB (2009) Anxiolytic and hypnotic effect of Crocus sativus aqueous extract and its constituents, crocin and safranal, in mice. Phytother Res 23:768–774

    Article  CAS  Google Scholar 

  • Hyldgaard M, Mygind T, Meyer RL (2012) Essential oils in food preservation: mode of action, synergies, and interactions with food matrix components. Front Microbiol 3:1–24

    Article  Google Scholar 

  • Karimi E, Oskoueian E, Hendra R, Jaafar HZE (2010) Evaluation of Crocus sativus L. stigma phenolic and flavonoid compounds and its antioxidant activity. Mol 15:6244–6256

    Article  CAS  Google Scholar 

  • Kühn J, Zhu XQ, Considine T, Singh H (2007) Binding of 2-nonanone and milk proteins in aqueous model systems. J Agri Food Chem 55:3599–3604

    Article  Google Scholar 

  • Kühn J, Considine T, Singh H (2008) Binding of flavor compounds and whey protein isolate as affected by heat and high pressure treatments. J Agri Food Chem 56:10218–10224

    Article  Google Scholar 

  • Kure CF, Skaar I (2000) Mould growth on the Norwegian semi-hard cheeses Norvegia and Jarlsberg. Int J Food Microbiol 62:133–137

    Article  CAS  Google Scholar 

  • Licón CC, Carmona M, Llorens S, Berruga MI, Alonso GL (2010) Potential healthy effects of saffron spice (Crocus sativus L. stigmas) consumption. Functional Plant Sci Biotechnol 4:64–73

    Google Scholar 

  • Licón CC, Carmona M, Rubio R, Molina A, Berruga MI (2012a) Preliminary study of saffron (Crocus sativus L. stigmas) color extraction in a dairy matrix. Dyes Pigments 92:1355–1360

    Article  Google Scholar 

  • Licón CC, Hurtado De Mendoza J, Maggi L, Berruga MI, Martín Aranda RM, Carmona M (2012b) Optimization of headspace sorptive extraction for the analysis of volatiles in pressed ewes' milk cheese. Int Dairy J 23:53–61

    Article  Google Scholar 

  • Montagna MT, Santacroce MP, Spilotros G, Napoli C, Minervini F, Papa A, Dragoni I (2004) Investigation of fungal contamination in sheep and goat cheese in southern Italy. Mycopathologia 158:245–249

    Article  CAS  Google Scholar 

  • Neaves P, Williams AP (2012) Microbiological surveillance and control in cheese manufacture. In: Law BA, Tamine AY (eds) Technology of cheesemaking. Wiley, Oxford, pp 384–412

    Google Scholar 

  • Pintado C, De Miguel A, Acevedo O, Nozal L, Novella JL, Rotger R (2011) Bactericidal effect of saffron (Crocus sativus L.) on Salmonella enterica during storage. Food Control 22:638–642

    Article  CAS  Google Scholar 

  • Sarić ZO, Dizdarević TA (2011) Occurrence of yeast and moulds in cheese and measures for growth control. Prehrambena industrija-Mleko i Mlečni Proizvodi 1:8–11

    Google Scholar 

  • Sørhaug T (2011) Yeasts and molds. Spoilage molds. In: Fuquay JW (ed) Encyclopedia of dairy sciences 2nd edn. Academic Press, San Diego, pp 780–784

Download references

Acknowledgments

This work has been financially supported by the Consejería de Educación y Ciencia of the JCCM with Research Project PBI08-0245-8453 and funding for C. C. Licón. The authors wish to thank G. Alonso for his valuable advice while conducting the study and Julián Molero for his technical support.

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Correspondence to María I. Berruga.

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Celia M. Librán and Carmen C. Licón contributed equally to this work.

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Librán, C.M., Licón, C.C., Serrano-Díaz, J. et al. Safranal transference from ewe's milk to cheese and whey and antifungal properties of fortified whey. Dairy Sci. & Technol. 94, 83–89 (2014). https://doi.org/10.1007/s13594-013-0145-2

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  • DOI: https://doi.org/10.1007/s13594-013-0145-2

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