Dairy Science & Technology

, Volume 92, Issue 5, pp 439–453 | Cite as

Pink discolouration defect in commercial cheese: a review

  • D. F. M. Daly
  • P. L. H. McSweeney
  • J. J. Sheehan
Review Paper


Development of a pink color defect is an intermittent but persistent defect in a wide range of ripened cheese varieties (Swiss, Cheddar, Grana, and Italian types) which may or may not contain an added colorant, e.g., annatto. Pink discoloration results in downgrading or rejection of a product with consequential economic loss to producers. Pink discolorations can manifest in a number of ways, e.g., patches at the surface or within the cheese block, or as a uniform pink border occurring below the surface of cheese blocks. Little consensus exists as to what the discolorations and their underlying causes are. This review seeks to provide an overview and interpretation of the underlying factors associated with the defect for both research and commercial audiences. In cheeses without added colorant, pink discoloration has been associated with: certain strains of thermophilic lactobacilli and propionic acid bacteria, Maillard reactions, and microbial pigments (e.g., carotenoids and phenolic compounds), which may be responsible for development of pink-brown or dark brown discoloration. In cheeses with added colorant (usually annatto), the development of pink discoloration has been associated with: alteration of annatto colorant due to factors such as varying pH levels within the cheese matrix (particularly < pH 5.4), oxidation of bixin in storage under high intensity fluorescent lights in display cabinets, presence of oxygen, variations in redox potential, interactions between nitrates and annatto present in plastic surface coating and also due to interactions between colorants, and the use of high heat treatment during processed cheese manufacture.


Pink Discoloration Cheese Defect Annatto Pigment 



些含添加或不添加色素(如胭脂红)的成熟干酪(瑞士、切达、grana和意大利干酪)中出现的粉红色褪色问题是这类干酪经常出现的问题。粉红色褪色导致干酪产品质量下降,同时给生产商造成重大经济损失。粉红色褪色有多种表现形式,如干酪表面出现斑块、内部有断块,干酪断块表面下部边缘出现不均匀的粉红色。关于褪色以及褪色的原因目前还没达成共识。本文概述并诠释影响粉红色褪色的一些相关因素。对于未添加色素的干酪,粉红色褪色与某些嗜热乳酸菌、丙酸菌;美拉德反应和微生物色素(例如类胡萝卜素和酚类化合物)有关,这些因素可能是引起粉褐色或暗褐色褪色的原因。对于添加色素(如胭脂红)的干酪,粉红色褪色与胭脂红颜色的变化有关,而引起该变化的因素有干酪基质pH值的变化(特别是pH值<5.4) ; 干酪在存储和销售过程中高强度荧光造成的胭脂素氧化作用;氧气的存在;氧化还原电位的变化;硝酸盐和胭脂红在塑料包装纸下的相互作用,也可能是由于色素间的相互作用以及干酪生产过程中的高温处理而引起的。


粉红色 褪色 干酪 质量缺陷 胭脂红 色素 



This project was funded by the Irish Co-operative Organisation Society, Ltd.


  1. Aksu Z, Tuğba Eren A (2005) Carotenoids production by the yeast Rhodotorula mucilaginosa: use of agricultural wastes as a carbon source. Process Biochem 40:2985–2991CrossRefGoogle Scholar
  2. Andersen CM, Wold JP, Mortensen G (2006) Light-induced changes in semi-hard cheese determined by fluorescence spectroscopy and chemometrics. Int Dairy J 16:1483–1489CrossRefGoogle Scholar
  3. Anonymous (2000) Keeping cheese out of the pink. Food Product Design (February) 9:23Google Scholar
  4. Asperger H (1986) Fermentierte milchprodukte und einflüsse von hygienekeimen—2 Teil: Qualitätsfchler und verderben auf grund mikrobieller ursachen. Ernahrun/Nutrition 10:227–232Google Scholar
  5. Barnicoat CR (1937) The reactions and properties of annatto as a cheese colour, with particular reference to the chemistry of cheese discoloration. J Dairy Res 8:61–73CrossRefGoogle Scholar
  6. Barnicoat CR (1950) Cheese discoloration: oxidation of bixin in annatto-coloured cheeses promoted by sulphydryl compounds. J Dairy Res 17:209–213CrossRefGoogle Scholar
  7. Betzold N (2004) Pink discoloration of mozzarella cheese. Thesis, University of Wisconsin, WisconsinGoogle Scholar
  8. Bley ME, Johnson ME, Olson NF (1985) Factors affecting nonenzymatic browning of process cheese. J Dairy Sci 68:555–561CrossRefGoogle Scholar
  9. Bockelmann W (1997) Surface-ripened cheeses. In: 5th International Cheese Symposium, Cork, Ireland pages 121–132Google Scholar
  10. Bockelmann W, Hoppe-Seyler T (2001) The surface flora of bacterial smear-ripened cheeses from cow's and goat's milk. Int Dairy J 11:307–314CrossRefGoogle Scholar
  11. Bottazzi V, Cappa F, Scolari G, Parisi MG (2000) Occurring of pink discolouration in Grana cheese made with one-strain starter culture/Comparsa di colorazione rossa in formaggio Grana prodotto con starter monocoltura. Sci Tec Latt Casearia 51:67–74Google Scholar
  12. Brennan NM, Cogan TM, Loessner M, Scherer S (2004) In: Fox PF, McSweeney PLH, Cogan TM, Guinee TP (eds) Cheese: chemistry, physics and microbiology, Vol. 2: Major cheese groups 3rd edn. Elsevier. LondonGoogle Scholar
  13. Britton G, Hendry GA, Houghton JD (1996) Carotenoids, Natural food colorants. Chapman & Hall, New YorkCrossRefGoogle Scholar
  14. Carini S, Lodi R, Giussani G (1979) Pink discoloration of Fontina cheese .1. Microbial factors. Latte 4:914–920Google Scholar
  15. Carreira A, Paloma L, Loureiro V (1998) Pigment producing yeasts involved in the brown surface discoloration of ewes' cheese. Int J Food Microbiol 41:223–230CrossRefGoogle Scholar
  16. Chang SF, Ayres JW, Sandine WE (1985) Analysis of cheese for histamine, tyramine, tryptamine, histidine, tyrosine, and tryptophan. J Dairy Sci 68:2840–2846CrossRefGoogle Scholar
  17. Colchin LM, Owens SL, Lyubachevskaya G, Boyle-Roden E, Rankin SA (2001) Modified atmosphere packed Cheddar cheese shreds: influence of fluorescent light exposure and gas type on color and production of volatile compounds. J Agric Food Chem 49:2277–2282CrossRefGoogle Scholar
  18. Delgado-Vargas F, Paredes-López O (1996) Carotenoids, in natural food colorants, Natural colorants for food and nutraceutical uses. CRC Press, New YorkGoogle Scholar
  19. Dincheva E (1979) Pink discoloration of Kachkaval cheese Khranitelna Promishlenost 28:30–31Google Scholar
  20. Dufossé L (2009) Pigments, Microbial. In: Schaechter M (ed) Encyclopedia of microbiology, 3rd edn. Academic, OxfordGoogle Scholar
  21. Dufossé L, de Echanove MC (2004) The last step in the biosynthesis of aryl carotenoids in the cheese ripening bacteria Brevibacterium linens ATCC 9175 (Brevibacterium aurantiacum subsp. nov.) involves a cytochrome P450-dependent monooxygenase. Food Res Int 38:967–973CrossRefGoogle Scholar
  22. Eliskases-Lechner F, Ginzinger W (1995) The yeast flora of surface-ripened cheeses. Milchwissenschaft 50:458–462Google Scholar
  23. Ferchichi M, Hemme D, Bouillanne C (1986) Influence of oxygen and pH on methanethiol production from l-methionine by Brevibacterium linens CNRZ 918. Appl Env Microbiol 51:725–729Google Scholar
  24. Forge M, Guiraud JP, Galzy P (1977) Study of a fault in manufacture of Roquefort cheese. Lait 57:24–36CrossRefGoogle Scholar
  25. Gabier AC, Gourdon P, Reitz J, Leveau JY, Bouix M (2005) Intracellular physiological events of yeast Rhodotorula glutinis during storage at +4 degrees C. Int J Food Microbiol 105:97–109CrossRefGoogle Scholar
  26. Galaup P, Flamin C, Carlet E, Dufossé L (2005) HPLC analysis of the pigments produced by the microflora isolated from the ‘Protected Designation of Origin’ French red-smear soft cheeses Munster, Epoisses, Reblochon and Livarot. Food Res Int 38:855–860CrossRefGoogle Scholar
  27. Gavrish EY, Krauzova VI, Potekhina NV, Karasev SG, Plotnikova EG, Altyntseva OV (2004) Three new species of brevibacteria, Brevibacterium antiquum sp. nov., Brevibacterium aurantiacum sp. nov., and Brevibacterium permense sp. nov. Micro 73:176–183CrossRefGoogle Scholar
  28. Giuliano G, Rosati C, Bramley PM (2003) To dye or not to dye: biochemistry of annatto unveiled. Trends Biotechnol 21:513–516CrossRefGoogle Scholar
  29. Govindarajan S, Morris HA (1973) Pink discoloration in Cheddar cheese. J Food Sci 38:675–678CrossRefGoogle Scholar
  30. Hong CM, Wendorff WL, Bradley RL (1995a) Effects of packaging and lighting on pink discoloration and lipid oxidation of annatto-colored cheeses. J Dairy Sci 78:1896–1902CrossRefGoogle Scholar
  31. Hong CM, Wendorff WL, Bradley RL (1995b) Factors affecting light-induced pink discoloration of annatto-colored cheese. J Food Sci 60:94–97CrossRefGoogle Scholar
  32. Juric M, Bertelsen G, Mortensen G, Petersen MA (2003) Light-induced colour and aroma changes in sliced, modified atmosphere packaged semi-hard cheeses. Int Dairy J 13:239–249CrossRefGoogle Scholar
  33. Kang EJ, Campbell RE, Bastian E, Drake MA (2010) Invited review: annatto usage and bleaching in dairy foods. J Dairy Sci 93:3891–3901CrossRefGoogle Scholar
  34. Kohl W, Achenbach H, Reichenbach H (1983) The pigments of Brevibacterium linens: aromatic carotenoids. Phytochemistry 22:207–210CrossRefGoogle Scholar
  35. Kosikowski FV (1982) In: Kosikowski F.V. and Assoc. (ed) Cheese and fermented milk Foods, 2nd edn. Brooktendale, New YorkGoogle Scholar
  36. Kristensen D, Orlien V, Mortensen G, Brockhoff P, Skibsted LH (2000) Light-induced oxidation in sliced Havarti cheese packaged in modified atmosphere. Int Dairy J 10:95–103CrossRefGoogle Scholar
  37. Kristensen D, Hansen CL, Arndal A, Trinderup RA, Skibsted LH (2001) Influence of light and temperature on the colour and oxidation stability of processed cheese. Int Dairy J 11:837–843CrossRefGoogle Scholar
  38. Leclercq-Perlat MN, Oumer A, Buono F, Bergere JL, Spinnler HE, Corrieu G (2000) Behavior of Brevibacterium linens and Debaryomyces hansenii as ripening flora in controlled production of soft smear cheese from reconstituted milk: protein degradation. J Dairy Sci 83:1674–1683CrossRefGoogle Scholar
  39. Lo TWC, Westwood ME, McLellan AC, Selwood T, Thornalley PJ (1994) Binding and modification of proteins by methylglyoxal under physiological conditions—a kinetic and mechanistic study with n-alpha-acetylarginine, n-alpha-acetylcysteine, and n-alpha-acetyllysine, and bovine serum-albumin. J Bio Chem 269:32299–32305Google Scholar
  40. Martley FG, Michel V (2001) Pinkish colouration in Cheddar cheese—description and factors contributing to its formation. J Dairy Res 68:327–332CrossRefGoogle Scholar
  41. McSweeney PLH (2007) In: McSweeney PLH (ed) Cheese problems solved. Woodhead, CambridgeGoogle Scholar
  42. Moir GM (1933) Discoloration in New Zealand Cheddar cheese. Muddy, bleached and pink defects. II. Biochemical investigations. J Dairy Res 4:238–245CrossRefGoogle Scholar
  43. Morgan GFV (1933) Discoloration in New Zealand Cheddar cheese. Muddy, pink and bleached defects. I. Bacteriological investigations. J Dairy Res 4:226–237CrossRefGoogle Scholar
  44. Mortensen G, Sorensen J, Stapelfeldt H (2002) Light-induced oxidation in semihard cheeses. Evaluation of methods used to determine levels of oxidation. J Agric Food Chem 50:4364–4370CrossRefGoogle Scholar
  45. Mortensen G, Bertelsen G, Mortensen BK, Stapelfeldt H (2004) Light-induced changes in packaged cheeses—a review. Int Dairy J 14:85–102CrossRefGoogle Scholar
  46. Najar SV, Bobbio FO, Bobbio PA (1988) Effects of light, air, anti-oxidants and pro-oxidants on annatto extracts (Bixa orellana). Food Chem 29:283–289CrossRefGoogle Scholar
  47. O'Connell JE, Fox PF (2001) Significance and applications of phenolic compounds in the production and quality of milk and dairy products: a review. Int Dairy J 11:103–120CrossRefGoogle Scholar
  48. Paramita A, Broome MC (2008) Pink discolouration in Romano style cheese—role of < alpha > -dicarbonyls and ammonia. Proceedings of the 5th IDF Symposium on Cheese Ripening, Bern, Switherland: 130Google Scholar
  49. Park HS, Reinbold GW, Hammond EG (1967) Role of Propionibacteria in split defect of Swiss cheese. J Dairy Sci 50:820–823CrossRefGoogle Scholar
  50. Pelaez C, Northolt MD (1988) Factors leading to pink discoloration of the surface of Gouda cheese. Neth Milk and Dairy J 42:323–336Google Scholar
  51. Piantanida L, Vallone L, Cantoni C (1996) Colorazioni superficiali del formaggio Taleggio. Industrie Alimentari 35:147–148Google Scholar
  52. Piergiovanni L, Prati F (1983) Non-enzymatic discoloration in processed cheese. Industrie Alimentari 22:497–497Google Scholar
  53. Scotter MJ, Castle L, Honeybone CA, Nelson C (2002) Method development and analysis of retail foods for annatto food colouring material. Food Additives and Contaminants 19:205–222CrossRefGoogle Scholar
  54. Seiler H (1986) Identification of cheese-smear coryneform bacteria. J Dairy Res 53:439–449CrossRefGoogle Scholar
  55. Shannon EL, Olson NF (1969) Rapid screening test to predict the tendency of lactic starter cultures to produce pink discoloration in Italian cheese. J Dairy Sci 52:1678–1680CrossRefGoogle Scholar
  56. Shannon EL, Olson NF, von Elbe JH (1966) Pink discoloration in Italian varieties of cheese (Abstr.). J Dairy Sci 49:711Google Scholar
  57. Shannon EL, Olson NF, von Elbe JH (1968) Pink discoloration in Italian varieties of cheese. J Dairy Sci 51:613–614CrossRefGoogle Scholar
  58. Shannon EL, Olson NF, von Elbe JH (1969) Effect of lactic starter culture on pink discoloration and oxidation-reduction potential in Italian cheese. J Dairy Sci 52:1557–1561CrossRefGoogle Scholar
  59. Shannon EL, Olson NF, Deibel RH (1977) Oxidative metabolism of lactic acid bacteria associated with pink discoloration in Italian cheese. J Dairy Sci 60:1693–1697CrossRefGoogle Scholar
  60. Sheehan JJ, Fenelon MA, Wilkinson MG, McSweeney PLH (2007a) Effect of cook temperature on starter and non-starter lactic acid bacteria viability, cheese composition and ripening indices of a semi-hard cheese manufactured using thermophilic cultures. Int Dairy J 17:704–716CrossRefGoogle Scholar
  61. Sheehan JJ, Oliveira JC, Kelly AL, Mc Sweeney PLH (2007b) Effect of cook temperature on primary proteolysis and predicted residual chymosin activity of a semi-hard cheese manufactured using thermophilic cultures. Int Dairy J 17:826–834CrossRefGoogle Scholar
  62. Shumaker EK, Wendorff WL (1998) Factors affecting pink discoloration in annatto-colored pasteurized process cheese. J Food Sci 63:828–831CrossRefGoogle Scholar
  63. Smith K (2004) Whey processing CDR technical review: bleaching. Madison: Center for Dairy Research, WisconsinGoogle Scholar
  64. Thomas MA (1969) Browning reaction in Cheddar cheese. Aust J Dairy Technol 24:185–189Google Scholar
  65. Trobetas A, Badeka A, Kontominas MG (2008) Light-induced changes in grated Graviera hard cheese packaged under modified atmospheres. Int Dairy J 18:1133–1139CrossRefGoogle Scholar
  66. Valdés-Stauber N, Scherer S, Seiler H (1997) Identification of yeasts and coryneform bacteria from the surface microflora of brick cheeses. Int J Food Microbiol 34:115–129CrossRefGoogle Scholar
  67. Wilkinson MG, Meehan HF, Guinee TP (2000) Cheese diversificaction into speciality cheeses: approaches and models in Ireland and New Zealand. Farm Food 101:19–24Google Scholar
  68. Zehren VL, Nusbaum DD (2000) Processed cheese, 2nd edn. Cheese Reporter, MadisonGoogle Scholar

Copyright information

© INRA and Springer-Verlag, France 2012

Authors and Affiliations

  • D. F. M. Daly
    • 1
  • P. L. H. McSweeney
    • 2
  • J. J. Sheehan
    • 1
  1. 1.Teagasc Food Research CentreCo. CorkIreland
  2. 2.School of Food and Nutritional SciencesUniversity CollegeCorkIreland

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