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Long-term fertilization with urban and animal wastes enhances soil quality but introduces pharmaceuticals and personal care products

Abstract

Applying organic wastes such as manure or sewage sludge on agricultural fields improves soil physical structure and fertility, while giving purpose to otherwise disposed and burned waste products. Yet, xenobiotics, and especially pharmaceuticals and personal care products, may be present in these wastes and thereby enter the soil ecosystem. To investigate their occurrence and their potential effect on soil fauna, the CRUCIAL experimental fields, Denmark, were used. Here we combine, for the first time, a chemical wide-scope suspect screening analysis with a study of soil nematode faunal response on soils, which were fertilized with cattle manure, sewage sludge, human urine, and mineral fertilizers over 14 years. The suspect screening analysis was performed with a nanoflow ultra-high performance liquid chromatography coupled with high-resolution tandem mass spectrometry. The abundance of soil indigenous nematodes was determined in each treatment at two time-points, followed by laboratory tests with the nematode species Caenorhabditis elegans to assess its reproduction capacity in the soils. A total of 12 pharmaceuticals and personal care products were identified as being up- or down-concentrated in the different treatments compared to controls (mineral fertilizers): 8 and 2 were found to be up-concentrated in sludge- and urine-treated soils, respectively. Our results, however, showed a significantly greater abundance of nematodes in soils amended with sludge and manure, while C. elegans had the lowest reproduction capacity in the controls. The reproduction capacity tests did not reveal any chronic toxicity. A principal component analysis showed a clear correlation between nematode abundance and soil organic matter, water content, nutrient contents, and porosity. Overall, urban and animal wastes enhanced soil quality. Pharmaceuticals were detected, but adverse effects on the measured endpoints could not be demonstrated. Although further investigations are needed to examine other associated risks, recycling nutrients from urban areas into fertilizers can contribute to a more sustainable fertilization strategy.

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Availability of data and material

The datasets generated and analyzed during the current study are available in the CRUCIAL-NEMATODES-2020 repository at https://doi.org/10.17894/ucph.cda2671e-34a9-4461-9b63-9710556919b1.

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Acknowledgements

This work was carried out with technical support from the Tåstrup campus of the University of Copenhagen. We would also like to acknowledge the help of Anja Weibel in carrying out the soil nematode extractions, Cephas Mwanasaka for his help and support in the field, as well as Sif Burlin Svendsen and Suman Kharel from Aarhus University for providing pharmaceutical standards.

Funding

The authors gratefully acknowledge financial support from the Aarhus University Research Foundation (AUFF-T-2017-FLS-7–4) and the Danish Environmental Protection Agency under research grant agreement (HITLIST, MST-667–00207).

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Contributions

Conceptualization: N.C, J.V, J.M., and M.H.; methodology: N.C, M.H, J.V., and T.K.O.G.; investigation: J.V. and T.K.O.G.; resources: N.C, M.H., and J.M.; writing: J.V., T.K.O.G., N.C, J.M., and M.H.

Corresponding author

Correspondence to Jeanne Vuaille.

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The authors declare no competing interests.

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Vuaille, J., Gravert, T.K.O., Magid, J. et al. Long-term fertilization with urban and animal wastes enhances soil quality but introduces pharmaceuticals and personal care products. Agron. Sustain. Dev. 42, 1 (2022). https://doi.org/10.1007/s13593-021-00726-8

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  • DOI: https://doi.org/10.1007/s13593-021-00726-8

Keywords

  • Fertilization
  • Urban wastes
  • Ecotoxicology
  • Nematodes
  • High-resolution mass spectrometry
  • Suspect screening