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Bioprotection and alternative fertilisation of petunia using mycorrhiza in a soilless production system

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Abstract

Ornamental crop production is increasing worldwide and petunia is nowadays a major bedding plant with high economic impact. To meet growing demands, a high production of quality nursery plants is needed. However, production in greenhouses is confronted by two main problems: fertiliser-induced salinity and pathogen attack like by Thielaviopsis basicola which both cause high losses. Moreover, there is increasing consumer demand for crops produced by environment-friendly practices. The application of arbuscular mycorrhizal (AM) fungi can present an appropriate approach to avoid excessive use of fertilisers and pesticides. Here, we report studies of the interaction between Petunia hybrida and the AM fungus Glomus mosseae in abiotic and biotic stress situations. Root colonisation by G. mosseae, although very low, was able to compensate phosphate deficiency in the substrate and resulted in increased plant dry matter, water and phosphorus content in the shoots. However, these positive effects were attenuated at high salt concentrations (abiotic stress). In contrast, disease symptoms caused by the root pathogen T. basicola (biotic stress) were significantly reduced in G. mosseae-colonised plants. Such protective effects were not achieved with the AM fungi Glomus intraradices and Gigaspora rosea, and a negative effect of the pathogen on root colonisation by G. intraradices was observed. This shows for the first time under nursery practice conditions that G. mosseae can compensate the negative impact of lower levels of phosphate fertilisation on plant growth in a soilless production system and induce bioprotection of petunia against T. basicola. Based on the different effects observed with the three AM fungi, petunia can provide an experimental system to define the molecular mechanisms underlying mycorrhiza-induced resistance against root pathogens.

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References

  • Al-Karaki G.N., Hammad R., Rusan M. (2001) Response of two tomato cultivars differing in salt tolerance to inoculation with mycorrhizal fungi under salt stress. Mycorrhiza 11, 43–47.

    Article  CAS  Google Scholar 

  • Bodker L., Kjoller R., Kristensen K., Rosendahl S. (2002) Interactions between indigenous arbuscular mycorrhizal fungi and Aphanomyces euteiches in field-grown pea. Mycorrhiza 12, 7–12.

    Article  PubMed  CAS  Google Scholar 

  • Breuillin F., Schramm J., Hajirezaei M., Ahkami A., Favre P., Druege U., Hause B., Bucher M., Kretzschmar T., Bossolini E., Kuhlemeier C., Martinoia E., Franken P., Scholz U., Reinhardt D. (2010) Phosphate systemically inhibits development of arbuscular mycorrhiza in Petunia hybrida and represses genes involved in mycorrhizal functioning. Plant J. 64, 1002–1017.

    Article  PubMed  CAS  Google Scholar 

  • Chavez W., Di Benedetto A., Civeira G., Lavado R. (2008) Alternative soilless media for growing Petunia hybrida and Impatiens wallerana: Physical behavior, effect of fertilization and nitrate losses. Bioresource Technol. 99, 8082–8087.

    Article  CAS  Google Scholar 

  • David C.H. (2008) Suppression of Thielaviopsis basicola by two fungicides applied to sandy loam soils in New Mexico. Plant Disease 64, 1011–1012.

    Google Scholar 

  • Dreistadt S.H. (2001) Integrated pest management for floriculture and nurseries. University of California, Davis, CA.

    Google Scholar 

  • Fakhro A., Andrade-Linares D.R., Susanne von Bargen S.; Bandte M., Büttner C., Grosch R., Schwarz D., Franken P. (2010) Impact of Piriformospora indica on tomato growth and on interaction with fungal and viral pathogens. Mycorrhiza 20, 191–200.

    Article  PubMed  Google Scholar 

  • Garmendia I., Goicoechea N., Aguirreolea J. (2004) Effectiveness of three Glomus species in protecting pepper (Capsicum annuum L.) against verticillium wilt. Biol. Control 31, 296–305.

    Article  Google Scholar 

  • Gaur A., Adholeya A. (2000) Growth and flowering in Petunia hybrida, Callistephus chinensis and Impatiens balsamina inoculated with mixed AM inocula or chemical fertilizers in a soil of low P fertility. Sci. Hort. 84, 151–162.

    Article  CAS  Google Scholar 

  • Gerats T., Vandenbussche M. (2005) A model system comparative for research: Petunia. Trends Plant Sci. 10, 251–256.

    Article  PubMed  CAS  Google Scholar 

  • Gianinazzi S., Gollotte A., Binet M.N., van Tuinen D., Redecker D., Wipf D. (2010) Agroecology: the key role of arbuscular mycorrhizas in ecosystem services. Mycorrhiza 20, 519–530.

    Article  PubMed  Google Scholar 

  • Grillas S., Lucas M., Bardopoulou E., Sarafopoulos S. (2001) Perlite based soilless culture systems: Current commercial applications and prospects. Acta Hort. 548, 105–113.

    CAS  Google Scholar 

  • Gruda N. (2009) Do soilless culture systems have an influence on product quality of vegetables? J. Appl. Bot. Food Qual. 82, 141–147.

    Google Scholar 

  • Huang Z., He C.X., He Z.Q., Zou Z.R., Zhang Z.B. (2010) The effects of arbuscular mycorrhizal fungi on reactive oxyradical scavenging system of tomato under salt tolerance. Agri. Sci. China 9, 1150–1159.

    Article  CAS  Google Scholar 

  • Jeffries P., Gianinazzi S., Perotto S., Turnau K., Barea J.M. (2003) The contribution of arbuscular mycorrhizal fungi in sustainable maintenance of plant health and soil fertility. Biol. Fert. Soil 37, 1–16.

    Google Scholar 

  • Johnson J. (1916) Host plants of Thielavia basicola. J. Agri. Res. 7, 289–300.

    Google Scholar 

  • Kang J.G., van Iersel M.W. (2009) Managing fertilization of bedding plants: A comparison of constant fertilizer concentrations versus constant leachate electrical conductivity. Hortscience 44, 151–156.

    Google Scholar 

  • Latef A.A.H.A., Chaoxing H. (2011) Effect of arbuscular mycorrhizal fungi on growth, mineral nutrition, antioxidant enzymes activity and fruit yield of tomato grown under salinity stress. Sci. Hort. 127, 228–233.

    Article  Google Scholar 

  • Lerat S., Lapointe L., Gutjahr S., Piche Y., Vierheilig H. (2003) Carbon partitioning in a split-root system of arbuscular mycorrhizal plants is fungal and plant species dependent. New Phytol. 157, 589–595.

    Article  Google Scholar 

  • Oyarzun P., Gerlagh M., Hoagland A.E. (1993) Pathogenic fungi involved in root-rot of peas in the Netherlands and their physiological specialization. Neth. J. Plant Pathol. 99, 23–33.

    Article  Google Scholar 

  • Paulitz T.C., Belanger R.R. (2001) Biological control in greenhouse systems. Annu. Rev. Phytopathol. 39, 103–133.

    Article  PubMed  CAS  Google Scholar 

  • Phillips J.M., Hayman D.S. (1970) Improved procedures for clearing roots and staining parasitic and vesicular-arbuscular mycorrhizal fungi for rapid assessment of infection. Transact. Brit. Mycol. Soc. 55, 158–161.

    Article  Google Scholar 

  • Pozo M.J., Azcon-Aguilar C., Dumas-Gaudot E., Barea J.M. (1999) [beta]-1,3-Glucanase activities in tomato roots inoculated with arbuscular mycorrhizal fungi and/or Phytophthora parasitica and their possible involvement in bioprotection. Plant Sci. 141, 149–157.

    Article  CAS  Google Scholar 

  • Rosendahl C.N., Rosendahl S. (1990) The role of vesicular-arbuscular mycorrhiza in controlling damping-off and growth reduction in cucumber caused by Pythium ultimum. Symbiosis 9, 363–366.

    Google Scholar 

  • Ruiz-Lozano J.M., Azcon R. (2000) Symbiotic efficiency and infectivity of an autochthonous arbuscular mycorrhizal Glomus sp from saline soils and Glomus deserticola under salinity. Mycorrhiza 10, 137–143.

    Article  CAS  Google Scholar 

  • Schneider J.H.M., Salazar O., Rubio V., Keijer J. (1997) Identification of Rhizoctonia solani associated with field-grown tulips using ITS rDNA polymorphism and pectic zymograms. Europ. J. Plant Pathol. 103, 607–622.

    Article  CAS  Google Scholar 

  • Shamshiri M.H., Mozafari V., Sedaghati E., Bagheri V. (2011) Response of petunia plants (Petunia hybrida cv. Mix) inoculated with Glomus mosseae and Glomus intrarardices to phosphorus and drought stress. J. Agri. Sci. Technol. 13, 929–942.

    Google Scholar 

  • Smith S.E., Read D.J. (2008) Mycorrhizal symbiosis. Academic Press, London.

    Google Scholar 

  • Trouvelot A., Kough J.L., Gianinazzi-Pearson V. (1986) Mesure du taux de mycorhization VA d’un système radiculaire. Recherche des méthodes d’estimation ayant une signification fonctionnelle. in: Gianinazzi-Pearson V., Gianinazzi S. (Eds.), The Mycorrhizae : Physiology and Genetic, INRA Presse, Paris, pp. 217–221.

    Google Scholar 

  • Wegmueller S., Svistoonoff S., Reinhardt D., Stuurman J., Amrhein N., Bucher M. (2008) A transgenic dTph1 insertional mutagenesis system for forward genetics in mycorrhizal phosphate transport of Petunia. Plant J. 54, 1115–1127.

    Article  Google Scholar 

  • Whipps JM (1997) Developments in the biological control of soil-borne plant pathogens. In: Callow JA (ed) Advances in botanical research. Academic Press, New York, pp 1–134

    Google Scholar 

Download references

Acknowledgements

We like to thank Uwe Drüge and Yvonne Klopotek for helping with setting up the experimental systems. The project was supported by the Ministries of Consumer Protection, Food and Agriculture of the Federal Republic of Germany, of the Land Brandenburg and of the Land Thüringen and by the Burgundy Regional Council (PhD grant to S. Hayek).

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Authors and Affiliations

  1. Leibniz-Institute of Vegetable and Ornamental Crops, Theodor-Echtermeyer-Weg 1, 14979, Grossbeeren, Germany

    Soukayna Hayek, Rita Grosch & Philipp Franken

  2. UMR INRA/CNRS/Université de Bourgogne, Plante-Microbe-Environnement, BP 86510, 21065, Dijon, France

    Soukayna Hayek & Vivienne Gianinazzi-Pearson

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  1. Soukayna Hayek
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  2. Rita Grosch
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  3. Vivienne Gianinazzi-Pearson
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  4. Philipp Franken
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Correspondence to Philipp Franken.

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Hayek, S., Grosch, R., Gianinazzi-Pearson, V. et al. Bioprotection and alternative fertilisation of petunia using mycorrhiza in a soilless production system. Agron. Sustain. Dev. 32, 765–771 (2012). https://doi.org/10.1007/s13593-012-0083-z

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  • DOI: https://doi.org/10.1007/s13593-012-0083-z

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