A scientific note on using large mixed sperm samples in instrumental insemination of honeybee queens
Keywordsartificial insemination bee breeding Apis mellifera homogenous mixing genetic diversity
Financial support was granted by the Brandenburg State Agency for Rural Development, Agriculture and Land Consolidation to the HOSIMET (Homogenous sperm mixing technology in Europe-wide Varroa Tolerance Breeding) breeding program (State association of Buckfast beekeepers Berlin-Brandenburg e.V.). Further, we thank C. Orzessek and P. Leibe for help with the lab work, and H.M.G. Lattorff for sharing data on primer polymorphism and heterozygosity across several Apis mellifera sub-species.
JPvP, JB, RFAM and SE conceived this research; JTP, RFAM and SE interpreted the data; JTP and SE performed experiments and analysis; All authors wrote the paper and participated in the revisions of it.
- Brauße, J., van Praagh, J.P. (2010) Stirring large volumes of pooled honeybee semen. Proc. Neth. Entomol. Soc. Meet. 21, 49–53Google Scholar
- Cobey, S.W., Tarpy, D.R., Woyke, J. (2013) Standard methods for instrumental insemination of Apis mellifera queens, In: Dietemann, V., Ellis, J.D. and Neumann, P. (Eds.), The COLOSS BEEBOOK, Volume I: Standard methods for Apis mellifera research. J. Apic. Res. 52, 1–18, DOI: 10.3896/IBRA.1.52.4.09
- van Praagh, J.P., Brauße, J., Titera, D. (2014) Mixing and storing large volumes of honeybee (Apis mellifera mellifera L.) sperm integrated in a breeding program. Proc. Neth. Entomol. Soc. Meet., 25, 39–45Google Scholar