, Volume 48, Issue 5, pp 716–718 | Cite as

A scientific note on using large mixed sperm samples in instrumental insemination of honeybee queens

  • Johanna T. Pieplow
  • Jürgen Brauße
  • Jacob P. van Praagh
  • Robin F. A. Moritz
  • Silvio Erler
Scientific note


artificial insemination bee breeding Apis mellifera homogenous mixing genetic diversity 



Financial support was granted by the Brandenburg State Agency for Rural Development, Agriculture and Land Consolidation to the HOSIMET (Homogenous sperm mixing technology in Europe-wide Varroa Tolerance Breeding) breeding program (State association of Buckfast beekeepers Berlin-Brandenburg e.V.). Further, we thank C. Orzessek and P. Leibe for help with the lab work, and H.M.G. Lattorff for sharing data on primer polymorphism and heterozygosity across several Apis mellifera sub-species.


JPvP, JB, RFAM and SE conceived this research; JTP, RFAM and SE interpreted the data; JTP and SE performed experiments and analysis; All authors wrote the paper and participated in the revisions of it.

Supplementary material

13592_2017_516_MOESM1_ESM.pdf (169 kb)
ESM 1 (PDF 169 kb)


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Copyright information

© INRA, DIB and Springer-Verlag France 2017

Authors and Affiliations

  1. 1.Institut für Biologie, Molekulare ÖkologieMartin-Luther-Universität Halle-WittenbergHalle (Saale)Germany
  2. 2.TrebbinGermany
  3. 3.CelleGermany
  4. 4.Department of Zoology and EntomologyUniversity of PretoriaPretoriaSouth Africa
  5. 5.German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-LeipzigLeipzigGermany

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