Apis mellifera populations are being threatened by several pathogens and parasitosis. Several authors have proposed that honey bee colonies may suffer from a compromised immune system leading to colony loss. This is why the study of A. mellifera immune system has become a topic of pressing concern. Nitric oxide (NO) is a signaling and immune effector molecule that has been proposed as a key molecule in invertebrate immunity, and that plays a part in A. mellifera cellular defenses. This paper deals with NO participation in the response to wounding/encapsulation challenge in A. mellifera fifth instar (L5) larvae. Challenging A. mellifera L5 larvae with nylon implants enhanced NO production and spreading in granulocyte-like hemocytes and increased the number of this NO-producing hemocyte type. However, AmNOS expression levels were not influenced by the insult. These results reveal that NO participates in the wound healing/encapsulation response as a signal molecule, possibly by the activation of a constitutively expressed AmNOS in honey bees.
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This research was supported by PICT-2012-0594 granted by the “Agencia Nacional de Promoción Científica y Tecnológica” to M.E. The authors would like to thank CONICET and the UNMdP. Pedro Negri is a doctoral fellow from CONICET, Argentina.
Manuscript editor: Klaus Hartfelder
Les hémocytes d’Apis mellifera produisent des quantités accrues d’oxyde nitrique en réponse à une blessure/encapsulation
Abeille domestique/larve/défense immunitaire/réaction de défense
Als Antwort auf eine Verwundungs/Einschlussreaktion produzieren die Hämozyten von Apis mellifera erhöhte Mengen an Stickoxid
M. Eguaras and L. Lamattina contributed equally as senior authors.
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Negri, P., Quintana, S., Maggi, M. et al. Apis mellifera hemocytes generate increased amounts of nitric oxide in response to wounding/encapsulation. Apidologie 45, 610–617 (2014). https://doi.org/10.1007/s13592-014-0279-0