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Apidologie

, Volume 42, Issue 6, pp 749–758 | Cite as

Ovary growth and protein levels in ovary and fat body during adult-wintering period in the red mason bee, Osmia rufa

  • Oskar Wasielewski
  • Karol GiejdaszEmail author
  • Tatiana Wojciechowicz
  • Marek Skrzypski
Original article

Abstract

Winter months are supposed to be a crucial period for the development of the reproductive system, especially ovaries, in Osmia rufa females. During 7 months of the wintering period (from September till March), the length and width of terminal oocytes and protein concentrations in ovary and fat body tissue extracts of red mason bee females, O. rufa, were studied. Our observations indicate statistically significant differences in the size of terminal oocytes for three different periods of over-wintering: September–October, November–January and February–March. Ovary growth was fast in the September–October period; it slowed down in November–January, and then increased again in February–March. Time-dependent changes were noted also in the number of oocytes and protein concentrations in ovary and fat body tissue during all wintering periods. During all winter months the number of oocytes progressively increased, but a statistically significant increase was only noticed for the periods September–October and February–March. Similarly, during the first two months (September–October) and the last three (February–April) we observed major changes in protein concentration in ovary and fat body tissue. Our results indicate that adult diapause in O. rufa varies to some extent in comparison to the other insects, probably because the the ovary is not fully inhibited during wintering, including the diapause period. These results allow a better understanding of the wintering strategies, including the diapause period, and may be useful for improving rearing methods of this economically important species.

Keywords

diapause fat body Osmia rufa ovary over-wintering proteins solitary bee 

Notes

Acknowledgements

This study was supported by grant no. N N311 298935 from the Ministry of Science and Higher Education. The authors would like to thank T. H. Sparks and P. Skórka for their constructive criticism and linguistic corrections of this manuscript.

Contract grant sponsor: Grant no. N N311 298935 from Ministry of Science and Higher Education, Poland

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Copyright information

© INRA, DIB-AGIB and Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Oskar Wasielewski
    • 1
  • Karol Giejdasz
    • 1
    Email author
  • Tatiana Wojciechowicz
    • 2
  • Marek Skrzypski
    • 2
  1. 1.Institute of ZoologyPoznań University of Life SciencesPoznańPoland
  2. 2.Department of Animal Physiology and BiochemistryPoznań University of Life SciencesPoznańPoland

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