Abstract
This study was conducted to improve the knowledge about bacteria associated with honey bees, Apis mellifera carnica. In this survey, the diversity of Gram-negative non-fermenting bacteria isolated and cultivated from pollen loads, honey sac, freshly stored nectar, and honey was investigated. Bacteria were characterized by a polyphasic approach. Based on morphological and physiological characteristics and comparison of isolates protein patterns after sodium dodecyl sulfate–polyacrylamide gel electrophoresis, 11 protein similarity groups were established and confirmed by enterobacterial repetitive intergenic consensus PCR. One isolate, representing a protein similarity group (representative strain), was characterized in more detail by analysis of respiratory quinones, amplified ribosomal DNA restriction analysis, and 16S rDNA sequence analysis. Based on the results of these examinations, seven representative strains were identified as members of the genus Pseudomonas. The remaining representative strains were allocated to the genera Acinetobacter, Chryseobacterium, Stenotrophomonas, and Commamonas.
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Acknowledgements
This work was supported in part by internal funds of the Institute of Bacteriology, Mycology and Hygiene, University of Veterinary Medicine Vienna, Institute for Apiculture, Austrian Agency for Health and Food Safety as well as Clinic for Avian, Reptile and Fish Medicine, University of Veterinary Medicine Vienna. We thank Gabriele Rothmüller, Irmgard Derakhshifar, Hermann Pechhacker, and especially Sandra Buczolits for helpful comments and suggestions and Christine Schramm and Katharina Etter for technical assistance.
Caractérisation de bactéries sélectionnées, à Gram-négatif, non fermentantes, associées aux abeilles ( Apis mellifera carnica )
Diversité bactérienne / PCR / miel / abeille / gène 16S rADN
Zusammenfassung – Charakterisierung Gram-negativer, nicht fermentierender Bakterien, die aus Honigbienen ( Apis mellifera carnica ) isoliert worden waren. Die vorliegende Studie wurde durchgeführt, um die Diversität der ausgewählten Gram-negativen, nicht fermentierenden Bakterien, die von Honigbienen (Apis mellifera carnica) gesammelten Pollenhöschen, dem Honigblaseninhalt, frisch eingetragenem Nektar (FSN) und Honig zu untersuchen. Die Diversität der Gram-negativen, nicht fermentierenden Bakterien wurde durch einen polyphasischen Ansatz (Kulturbedingungen, morphologische und biochemische Charakteristika; Vergleich der Proteinmuster der Isolate nach SDS-PAGE, ERIC-PCR; Analyse der respiratorischen Chinone, ARDRA und 16S rDNA Sequenzanalyse) charakterisiert. Dabei wurden aus den insgesamt 724 gewonnen Bakterienisolaten 104 Isolate als nicht-sporenbildende gram-negative Stäbchen charakterisiert, die aus Glucose keine Säure durch Gärung bilden konnten. Diese 104 Isolate wurden dann weiter untersucht. Nach dem Vergleich der mittels SDS-PAGE erhaltenen Proteinmuster wurden 11 Gruppen mit ähnlichem Proteinmuster gebildet, die durch ERIC-PCR bestätigt werden konnten. Aus jeder Gruppe mit ähnlichem Proteinmuster wurde je ein Isolat ausgewählt (= repräsentativer Stamm) und durch die Analyse der respiratorischen Chinone, ARDRA und 16S rDNA Sequenzanalyse näher untersucht. Ausgehend von den Ergebnissen dieser Untersuchungen konnten 7 der repräsentativen Stämme als Mitglieder der Gattung Pseudomonas identifiziert werden. Die verbleibenden repräsentativen Stämme wurden den Gattungen Acinetobacter, Chryseobacterium, Stenotrophomonas und Commamonas zugeordnet. Wie gezeigt werden konnte, sind Gram-negative nicht fermentierende Bakterien im Umfeld eines Bienenvolkes weit verbreitet. Somit könnten weiterführende Untersuchungen hinsichtlich des antagonistischen Potenzials dieser Bakterien gegenüber verschiedenen Krankheitserregern bei Bienen vielversprechend sein.
Bakteriendiversität / PCR / Honig / Honigbienen / 16S rDNA Gen
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Loncaric, I., Ruppitsch, W., Licek, E. et al. Characterization of selected Gram-negative non-fermenting bacteria isolated from honey bees (Apis mellifera carnica). Apidologie 42, 312–325 (2011). https://doi.org/10.1007/s13592-011-0019-7
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DOI: https://doi.org/10.1007/s13592-011-0019-7