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Reduction of allergy effects of peanut sprout extract in a systemic anaphylaxis food allergy mouse model

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Abstract

Peanut allergy is a major cause of food-induced anaphylaxis and subsequent fatality. Food allergies are becoming an increasingly important global health issue. This study was undertaken to verify the effects of peanut sprout extract (PNSE) on the inhibition of allergic and anaphylactic responses using a peanut (PN)-immunized food allergy mouse model. Fresh peanut sprouts were germinated for 0, 3, 5, and 7 days using mature peanuts. Mice were then sensitized to cholera toxin plus PNE or PNSE by intragastric administration on days 0 and 7, and were then challenged with PNE or PNSE on days 21 and 35. After 5 weeks, we examined the mucosal mast cell degranulation, ear swelling, and systemic anaphylaxis stimulated by PNE extract, in comparison with PNSE. Subsequently, Ara h1, a biomarker of PNE allergy; serum levels; and Th1/Th2 cytokine production in supernatants of cultured splenocytes were measured. PNSE treatment significantly attenuated the secretion of anti-Ara h1 antibody, mucosal mast cell degranulation, degree of systemic anaphylaxis, and ear swelling and increased the production of IFN-γ and IL-10, with a decrease in IL-4 secretion. The results of this study show that the allergenicity of PNE could be reduced by germination, which caused downregulation of Th2 lymphocyte activity, systemic anaphylactic response, and mast cell-mediated ear swelling in PNE-sensitized mice.

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Acknowledgements

This study was supported by a grant from the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry (IPET) through Technology Commercialization Support Program, funded by the Ministry of Agriculture, Food and Rural Affairs (MAFRA) (817036-03), the Korea Technology and Information Promotion Agency for SMEs in Korea small business innovation research program (KOSBIR) (P0010163) and the Basic Science Research Program (NRF-2020R1A2C1015119) through the National Research Foundation (NRF) of Korea funded by the Ministry of Science and ICT.

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Authors and Affiliations

  1. Institute of Traditional Medicine and Bioscience, Daejeon University, 62 Daehak-ro, Dong-gu, Daejeon, 34520, Republic of Korea

    Won-Kyung Yang, Yang-Chun Park & Seung-Hyung Kim

  2. Division of Respiratory System, Department of Internal Medicine, College of Korean Medicine, Institute of Traditional Medicine and Bioscience, Daejeon University, Daejeon, Korea

    Won-Kyung Yang & Yang-Chun Park

  3. KM Convergence Research Division, Korea Institute of Oriental Medicine, Daejeon, Korea

    Dong-Seon Kim, Eunjung Son & Yun Mi Lee

  4. Department of Horticulture, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon, 34134, Korea

    Geung-Joo Lee

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  1. Won-Kyung Yang
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Contributions

YWK analysis, statistical analysis, writing of the manuscript. KDS manuscript corrections. SEJ, LYM experiment execution, extraction. KSH mentor, the experiment initiator and manuscript supervisor. PYC, LGJ results review and comment, manuscript supervisor.

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Correspondence to Seung-Hyung Kim.

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Communicated by Jeong Hee Choi.

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Yang, WK., Kim, DS., Son, E. et al. Reduction of allergy effects of peanut sprout extract in a systemic anaphylaxis food allergy mouse model. Hortic. Environ. Biotechnol. 62, 637–647 (2021). https://doi.org/10.1007/s13580-021-00336-z

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  • DOI: https://doi.org/10.1007/s13580-021-00336-z

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