This study selected Dendrobium officinale protocorms as the experimental material and used different light-quality treatments and calcium signalling inhibitors to explore the mechanism of the biological response of calcium to light quality. The results indicate that a 1:3 red:blue (R:B) light ratio promotes the accumulation of biomass and polysaccharides in the protocorm. Nevertheless, this effect can be entirely counteracted by calmodulin (CaM) inhibitors and calcium ion-chelating agents. The possible mechanisms of this effect are as follows: (1) The Ca2+-CaM signal-regulated photosynthetic apparatus is improved by the R:B 1:3 light, increasing the CaM content and Ca2+-ATPase activity. Thus, R:B 1:3 light increases the development of the photosynthetic apparatus in the D. officinale protocorm, enhances its photosynthetic productivity, and promotes the activities of sucrose phosphatase; accordingly, biomass and sucrose accumulation increase. (2) R:B 1:3 light enhances Ca2+-CaM signalling and regulates glycometabolic processes to promote the activities of sucrose synthase, thus increasing fructose and glucose levels and providing sufficient synthetic precursors to promote polysaccharide production.
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The datasets generated during and analyzed during the current study are available from the corresponding author on reasonable request.
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This work was supported by the National Key R&D Program of China (NO.2018YFD0600105), Sichuan Science and Technology Program (NO. 2018JY0211), and Sichuan Landscape and Recreation Research Center (JGYQ2019024).
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Communicated by So-Young Park, Ph.D.
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Lei, T., Gao, S., Lin, X. et al. Calcium signalling mediated the regulation of growth and polysaccharide accumulation by light quality in Dendrobium officinale protocorms. Hortic. Environ. Biotechnol. (2021). https://doi.org/10.1007/s13580-020-00304-z
- Calcium signal
- Dendrobium officinale
- Light quality