Privet golden leaves adapt unexpectedly well to light changes

Abstract

Golden-leaf privet (Ligustrum × vicaryi) is widely used as a horticultural shrub because of its upper golden leaves, but its lower leaves are green. However, the putative mechanisms of its upper golden leaves and the leaf color changes in response to light shifts have not been well studied so far. Here, chlorophylls (Chl), carotenoids, and Chl precursors from both golden and green leaves grown in full sunlight (approximately 1200 μmol photons m−2 s−1 at noon) or low-light conditions (180 μmol m−2 s−1) were determined spectrophotometrically. In addition, their gas exchange parameters and Chl fluorescence were measured in situ. Metabolic flux analysis of chlorophyll intermediates indicated that the conversion of prochlorophyllide to chlorophyllide was significantly blocked in golden leaves when compared with green leaves. Green leaves showed higher photosynthetic capacity in low light than golden leaves, but golden leaves presented unexpectedly stronger photosynthetic capacity and lower reactive oxygen species accumulation under the high-light condition. Furthermore, golden leaves showed a higher level of nonphotochemical quenching (NPQ) after the light-to-dark shift and presented a stronger adaptive ability to a broad range of light environments. Higher NPQ values and less oxidative damage in golden leaves may be correlated with their higher carotenoid levels. The results imply that lower chlorophyll levels and higher carotenoid levels in canopy leaves may help privet plants acclimate better to illumination changes. This study demonstrates the key role of irradiance in generating the two types of Ligustrum × vicaryi leaves and sheds a light on cultivation of other ornamental foliage plants.

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References

  1. Ahn TK, Avenson TJ, Ballottari M, Cheng YC, Niyogi KK, Bassi R, Fleming GR (2008) Architecture of a charge-transfer state regulating light harvesting in a plant antenna protein. Science 320:794–797

    CAS  Article  Google Scholar 

  2. Bogorad L (1962) Porphyrin synthesis. Method Enzymol 5:885–891

    CAS  Article  Google Scholar 

  3. Chen YE, Cui JM, Li GX, Yuan M, Zhang DW, Yuan S, Zhang HY (2016) Effect of salicylic acid on the antioxidant system and photosystem II in wheat seedlings. Biol Plant 60:139–147

    CAS  Article  Google Scholar 

  4. Chen YE, Zhang CM, Su YQ, Ma J, Zhang ZW, Yuan M, Zhang HY, Yuan S (2017) Responses of photosystem II and anti-oxidative systems to high light and high temperature co-stress in wheat. Environ Exp Bot 135:45–55

    CAS  Article  Google Scholar 

  5. Dei M (1985) Benzyladenine-induced stimulation of 5-aminolevulinic acid accumulation under various light intensities in levulinic acid-treated cotyledons of etiolated cucumber. Physiol Plant 64:153–160

    CAS  Article  Google Scholar 

  6. Espineda CE, Linford AS, Devine D, Brusslan JA (1999) The AtCAO gene, encoding chlorophyll a oxygenase, is required for chlorophyll b synthesis in Arabidopsis thaliana. Proc Natl Acad Sci USA 96:10507–10511

    CAS  Article  Google Scholar 

  7. Holt NE, Zigmantas D, Valkunas L, Li XP, Niyogi KK, Fleming GR (2005) Carotenoid cation formation and the regulation of photosynthetic light harvesting. Science 307:433–436

    CAS  Article  Google Scholar 

  8. Kami C, Lorrain S, Hornitschek P, Fankhauser C (2010) Light-regulated plant growth and development. Curr Top Dev Biol 91:29–66

    CAS  Article  Google Scholar 

  9. Krause GH, Weis E (1991) Chlorophyll fluorescence and photosynthesis: the basics. Annu Rev Plant Physiol Plant Mol Biol 42:313–349

    CAS  Article  Google Scholar 

  10. Lichtenthaler HK, Wellburn AR (1983) Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochem Soc Trans 603:591–592

    Article  Google Scholar 

  11. Liu ZL, Yuan S, Liu WJ, Du JB, Tian WJ, Luo MH, Lin HH (2008) Mutation mechanism of chlorophyll-less barley mutant NYB. Photosynthetica 46:73–78

    CAS  Article  Google Scholar 

  12. Masuda T, Fujita Y (2008) Regulation and evolution of chlorophyll metabolism. Photochem Photobiol Sci 7:1131–1149

    CAS  Article  Google Scholar 

  13. Meskauskiene R, Nater M, Goslings D, Kessler F, op den Camp R, Apel K (2001) FLU: a negative regulator of chlorophyll biosynthesis in Arabidopsis thaliana. Proc Natl Acad Sci USA 98:12826–12831

    CAS  Article  Google Scholar 

  14. Pattanayak GK, Biswal AK, Reddy VS, Tripathy BC (2005) Light-dependent regulation of chlorophyll b biosynthesis in chlorophyllide a oxygenase overexpressing tobacco plants. Biochem Biophys Res Commun 326:466–471

    CAS  Article  Google Scholar 

  15. Pawłowska B, Żupnik M, Szewczyk-Taranek B, Cioć M (2018) Impact of LED light sources on morphogenesis and levels of photosynthetic pigments in Gerbera jamesonii grown in vitro. Hortic Environ Biotech 59:115–123

    Article  Google Scholar 

  16. Porra RJ, Thompson WA, Kriedemann PE (1989) Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectroscopy. Biochim Biophys Acta Bioenergetics 975:384–394

    CAS  Article  Google Scholar 

  17. Rebeiz CA (2002) Analysis of intermediates and end products of the chlorophyll biosynthetic pathway. In: Smith AG, Witty M (eds) Heme, chlorophyll, and bilins: methods and protocols. Springer, Totowa, pp 111–155

    Google Scholar 

  18. Reinbothe C, Lebedev N, Reinbothe S (1999) A protochlorophyllide light-harvesting complex involved in de-etiolation of higher plants. Nature 397:80–84

    CAS  Article  Google Scholar 

  19. Rüdiger W (2002) Biosynthesis of chlorophyll b and the chlorophyll cycle. Photosynth Res 74:187–193

    Article  Google Scholar 

  20. Tanaka A, Tanaka R (2006) Chlorophyll metabolism. Curr Opin Plant Biol 9:248–255

    CAS  Article  Google Scholar 

  21. Valladares F (2003) Light heterogeneity and plants: from ecophysiology to species coexistence and biodiversity. In: Esser K, Lüttge U, Beyschlag W, Hellwig F (eds) Progress in botany. Springer, Berlin, pp 439–471

    Google Scholar 

  22. Warpeha KM, Montgomery BL (2016) Light and hormone interactions in the seed-to-seedling transition. Environ Exp Bot 121:56–65

    CAS  Article  Google Scholar 

  23. Wilks A (2002) Analysis of heme and hemoproteins. In: Smith AG, Witty M (eds) Heme, chlorophyll, and bilins: methods and protocols. Springer, Totowa, pp 157–184

    Google Scholar 

  24. Yang Y, Qi M, Mei C (2004) Endogenous salicylic acid protects rice plants from oxidative damage caused by aging as well as biotic and abiotic stress. Plant J 40:909–919

    CAS  Article  Google Scholar 

  25. Yang YQ, Yi XF, Prasad P (2009) Response of photosynthesis and chlorophyll fluorescence quenching to leaf dichotocarpism in Ligustrum vicaryi, an ornamental herb. Photosynthetica 47:137–140

    CAS  Article  Google Scholar 

  26. Yuan M, Xu MY, Yuan S, Chen YE, Du JB, Xu F, Zhang ZW, Guo ZC, Zhao ZY, Lin HH (2010) Light regulation to chlorophyll synthesis and plastid development of the chlorophyll-less golden-leaf privet. J Integr Plant Biol 52:809–816

    CAS  Article  Google Scholar 

  27. Yuan M, Dong LH, Jia XJ, Yuan S, Du L (2014) Effects of canopy position on leaf structures in golden-leaf privet (Ligustrum × vicaryi). Bull Bot Res 34:188–193

    CAS  Google Scholar 

  28. Yuan M, Zhao YQ, Zhang ZW, Chen YE, Ding CB, Yuan S (2017) Light regulates transcription of chlorophyll biosynthetic genes during chloroplast biogenesis. Crit Rev Plant Sci 36:35–54

    Article  Google Scholar 

  29. Zha L, Liu W (2018) Effects of light quality, light intensity, and photoperiod on growth and yield of cherry radish grown under red plus blue LEDs. Hortic Environ Biotech 59:511–518

    CAS  Article  Google Scholar 

  30. Zhang DW, Yuan S, Xu F, Zhu F, Yuan M, Ye HX, Guo HQ, Lv X, Yin Y, Lin HH (2016) Light intensity affects chlorophyll synthesis during greening process by metabolite signal from mitochondrial alternative oxidase in Arabidopsis. Plant, Cell Environ 39:12–25

    Article  Google Scholar 

  31. Zhang ZW, Li MX, Huang B, Feng LY, Wu F, Fu YF, Zheng XJ, Peng HQ, Chen YE, Yang HN, Wu LT, Yuan M, Yuan S (2018) Nitric oxide regulates chlorophyllide biosynthesis and singlet oxygen generation differently between Arabidopsis and barley. Nitric Oxide 76:6–15

    CAS  Article  Google Scholar 

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (31770322) and the Project of Sichuan Province Youth Science and Technology Innovation Team (CN) (20CXTD0062). We would like to thank LetPub (www.letpub.com) for providing linguistic assistance during the preparation of this manuscript.

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SY and MY conceived the idea and designed the experiments; MY, BH, LHD, QHH and YY collected the data; CBD, CH, YEC and ZWZ analysed the data; MY and SY wrote of the manuscript. All authors gave final approval for publication.

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Correspondence to Shu Yuan.

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Communicated by Jongyun Kim, Ph.D.

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Yuan, M., Huang, B., Dong, LH. et al. Privet golden leaves adapt unexpectedly well to light changes. Hortic. Environ. Biotechnol. 61, 673–683 (2020). https://doi.org/10.1007/s13580-020-00254-6

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Keywords

  • Chlorophyll metabolism
  • Light shift
  • Ligustrum × vicaryi
  • Nonphotochemical quenching
  • Reactive oxygen species