Abstract
Vanda is an aerial tropical orchid native to Thailand and nitrogen (N) fertilizer is mainly used to promote its growth and quality. However, little is known about the characteristics of N absorption and assimilation in Vanda. The objective of this study was to determine the appropriate source of N for Vanda cultivation. In this experiment, shoots and roots of Vanda ‘Ratchaburi Fuchs-Katsura’ were sprayed weekly with 100 ml of 15N tracer solution (1) 10 mM of 15NO3−, (2) 5 mM of 15NO3− plus 5 mM of NH4+, (3) 5 mM of NO3− plus 5 mM of 15NH4+ and (4) 10 mM of 15NH4+. The results indicated that plants fed with a combined N fertilizer gave the highest of 15N use efficiency (15NUE) of about 21.8%, 30 days after the first feeding (DAF), compared with those fed sole sources of 15NO3− (21.0%) and 15NH4+ (16.6%). However, a sole nitrate fertilizer or combination fertilizer did not significantly affect the total N and labelled N content. Alanine was a major amino acid found in leaves and roots at 7 DAF, whereas glutamine was mainly found in stems. At 30 DAF, tyrosine and alanine became major components in the leaves, and glutamine decreased in stems when plants were fed with a single 15NH4+source.
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Acknowledgements
This work was supported by the Graduate School of Science and Technology at Niigata University and the Aksel T Schau Charitable Foundation. We acknowledge the Department of Environmental Science, the Faculty of Bioresource Sciences at Akita Prefectural University, and H.M. the King’s Initiative Centre for Flower and Fruit Propagation, Thailand for isotope analysis and facilities.
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Panjama, K., Ohyama, T., Ohtake, N. et al. Identifying N sources that affect N uptake and assimilation in Vanda hybrid using 15N tracers. Hortic. Environ. Biotechnol. 59, 805–813 (2018). https://doi.org/10.1007/s13580-018-0071-6
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DOI: https://doi.org/10.1007/s13580-018-0071-6