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Horticulture, Environment, and Biotechnology

, Volume 59, Issue 2, pp 263–274 | Cite as

Construction of genetic linkage maps of ‘Fina Sodea’ clementine (Citrus clementina) and Byungkyul (C. platymamma), a Korean landrace, based on RAPD and SSR markers

  • Yali Chang
  • Ho Bang Kim
  • Eun-Ui Oh
  • Kyunguk Yi
  • Kwan Jeong Song
Research Report Genetics and Breeding
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Abstract

In this study, genetic linkage maps were constructed for ‘Fina Sodea’ clementine (Citrus clementina) and Byungkyul (C. platymamma) using 152 F1 clones as a mapping population. Reference-guided whole-genome sequencing, using next-generation sequencing technology, along with 82 randomly amplified polymorphic DNA markers and seven genomic simple-sequence repeat (gSSR) loci, mined 41 polymorphic gSSR loci from the direct comparative analysis. The generated linkage map for ‘Fina Sodea’ clementine contained 22 markers distributed into 10 linkage groups, with a total length of 417.7 cM and an average interval of 19.0 cM. The map for Byungkyul contained 57 markers distributed into 10 linkage groups, with a total length of 684.9 cM and a smaller average interval of 12.0 cM. Comparison of shared gSSR markers between the two maps identified collinearities, such as linkage groups B3 and C7 and groups B9 and C7. The newly developed gSSR markers greatly enrich the limited amount of gSSR markers in Citrus, providing markers that can be useful for comparative mapping and other genetic studies.

Keywords

Citrus Genome mapping Genome sequence comparison Molecular markers 

Notes

Acknowledgements

This study was supported by Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) through Golden Seed Project (No. 213007-05-1-SBQ10), funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA).

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Copyright information

© Korean Society for Horticultural Science and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Yali Chang
    • 1
  • Ho Bang Kim
    • 2
  • Eun-Ui Oh
    • 1
  • Kyunguk Yi
    • 1
  • Kwan Jeong Song
    • 1
    • 3
  1. 1.Faculty of Bioscience and Industry, SARIJeju National UniversityJejuKorea
  2. 2.Life Science Research Institute, Biomedic Co., LtdBucheonRepublic of Korea
  3. 3.Research Institute for Subtropical Agriculture and BiotechnologyJeju National UniversityJejuRepublic of Korea

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