Horticulture, Environment, and Biotechnology

, Volume 58, Issue 6, pp 548–559 | Cite as

Seed germination, seedling growth and antioxidant system responses in cucumber exposed to Ca(NO3)2

Research Report

Abstract

This study investigated the effects of varying calcium nitrate (Ca(NO3)2) supply on seed germination, seedling growth, and antioxidant responses during cucumber seed germination. Five and 20 mM Ca(NO3)2 stimulated seed germination, while 10 and 40 mM Ca(NO3)2 inhibited it. Germinating seed weight was clearly promoted by 5 mM Ca(NO3)2, but decreased under 40 mM Ca(NO3)2. Ten or 20 mM Ca(NO3)2 caused no marked change. Addition of 10 or 40 mM Ca(NO3)2 increased the activity of many enzymes in germinating seeds, such as superoxide dismutases (SOD), peroxidases (POD), catalase (CAT), ascorbate peroxidase (APX), glutathione reductase (GR), dehydroascorbate reductase (DHAR), and monodehydroascorbate reductase (MDHAR). On the other hand, 5 and 20 mM Ca(NO3)2 markedly decreased CAT activity. Among all the treatments, only 10 mM Ca(NO3)2 increased malondialdehyde content. Similarly, the production rate of O2.− was only higher in 20 mM Ca(NO3)2. Compared with the control (0 mM Ca(NO3)2), protein content significantly increased in all treatments except for 20 mM Ca(NO3)2. Calcium nitrate strongly inhibited the growth of seedlings, and damaged leaf and root microstructure. The inhibition and damage were more severe as the Ca(NO3)2 concentration increased. Calcium nitrate promoted the accumulation of photosynthetic pigment, but led to a decrease in chlorophyll a/b. These results suggest that the effect of different Ca(NO3)2 levels on seed germination was variable, while the inhibition effect on seedling growth enhanced with increase of Ca(NO3)2 concentration. This effect is closely associated with Ca 2+ and NO3- concentration, antioxidant enzyme activity, and the different growth and development stages of cucumber.

Additional key words

antioxidantive enzymes Cucumis sativus germination rate biomass production secondary salinization 

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References

  1. Almansouri M, Kinet J-M, Lutts S (2001) Effect of salt and osmotic stresses on germination in durum wheat (Triticum durum Desf.). Plant Soil 231:243–254CrossRefGoogle Scholar
  2. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of proteindye binding. Anal Biochem 72:248–254CrossRefPubMedGoogle Scholar
  3. Cakmak I, Marschner H (1992) Magnesium deficiency and high light intensity enhance activities of superoxide dismutase, ascrobate peroxidase, and glutathione reductase in bean leaves. Plant Physiol 98:1222–1227CrossRefPubMedPubMedCentralGoogle Scholar
  4. Ebert G, Eberle J, Ali-Dinar H, Lüdders P (2002) Ameliorating effects of Ca(NO3)2 on growth, mineral uptake and photosynthesis of NaCl-stressed guava seedlings (Psidium guajava L.). Sci Hortic 93:125–135CrossRefGoogle Scholar
  5. Egley GH, Paul JrRN, Vaughn KC, Duke SO (1983) Role of peroxidase in the development of water-impermeable seed coats in Sida spinosa L. Planta 157:224–232CrossRefPubMedGoogle Scholar
  6. Elstner EF, Heupel A (1976) Inhibition of nitrate formation from hydroxylammoniumchloride: a simple assay for superoxide dismutase. Anal Biochem 70:616–620CrossRefPubMedGoogle Scholar
  7. Fan HF, Du CX, Ding L, Xu YL (2013) Effects of nitric oxide on the germination of cucumber seeds and antioxidant enzymes under salinity stress. Acta Physiol Plant 35:2707–2719CrossRefGoogle Scholar
  8. Forde B, Lorenzo H (2001) The nutritional control of root development. Plant Soil 232:51–68CrossRefGoogle Scholar
  9. Foyer CH, Halliwell B (1976) The presence of glutathione and glutathione reductase in chloroplasts: a proposed role in ascorbic acid metabolism. Planta 133:21–25CrossRefPubMedGoogle Scholar
  10. Foyer CH, Noctor G (2005) Oxidant and antioxidant signalling in plants: a re-evaluation of the concept of oxidative stress in a physiological context. Plant Cell Environ 28:1056–1071CrossRefGoogle Scholar
  11. Foyer CH, Lelandais M, Kunert KJ (1994) Photooxidative stress in plants. Physiol Plantarum 92:696–717CrossRefGoogle Scholar
  12. Giannopotitis CN, Ries SK (1977) Superoxide dismutase I. occurrence in higher plants. Plant Physiol 59:309–314Google Scholar
  13. Greenway H, Munns R (1980) Mechanism of salt tolerance in nonhylophytes. Annu Rev Plant Phyiol 31:149–190CrossRefGoogle Scholar
  14. He FF, Chen Q, Jiang RF, Chen XP, Zhang FS (2007) Yield and nitrogen balance of greenhouse tomato (Lycopersium esculentum Mill.) with conventional and site-speci-c nitrogen management in Northern China. Nutr Cycl Agroecosys 77:1–14CrossRefGoogle Scholar
  15. Heath RL, Packer L (1968) Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125:189–198CrossRefPubMedGoogle Scholar
  16. Hossain MA, Nakano Y, Asada K (1984) Monodehydroascorbate reductase in spinach chloroplasts and its participation in the regeneration of ascorbate for scavenging hydrogen peroxide. Plant Cell Physiol 25:385–395Google Scholar
  17. Huang H, Song SQ (2013) Change in desiccation tolerance of maize embryos during development and germination at different water potential PEG-6000 in relation to oxidative process. Plant Physiol Biochem 68:61–70CrossRefPubMedGoogle Scholar
  18. Kim YH, Khan AL, Waqas M, Shim JK, Kim DH, Lee KY, Lee IJ (2014) Silicon application to rice root zone influenced the phytohormonal and antioxidant responses under salinity stress. J Plant Growth Regul 33:137–149CrossRefGoogle Scholar
  19. Levin SA, Mooney HA, Field C (1989) The dependence of plant root: shoot ratios on internal nitrogen concentration. Ann Bot London 64:71–75CrossRefGoogle Scholar
  20. Misra N, Dwivedi UN (2004) Genotypic difference in salinity tolerance of green gram cultivars. Plant Sci 166:1135–1142CrossRefGoogle Scholar
  21. Nakano Y, Asada K (1981) Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22:867–880Google Scholar
  22. Nelson PV, Kowalczyk W, Niedziela JrCE, Mingis NC, Swallow WH (2003) Effects of relative humidity, calcium supply, and forcing season on tulip calcium status during hydroponic forcing. Sci Hortic 98:409–422CrossRefGoogle Scholar
  23. Park H-J, Miura Y, Kawakita K, Yoshioka H, Doke N (1998) Physiological mechanisms of a sub-systemic oxidative burst triggered by elicitor-induced local oxidative burst in potato tuber slices. Plant Cell Physiol 39:1218–1225CrossRefGoogle Scholar
  24. Sánchez E, Rivero RM, Ruiz JM, Romero L (2004) Changes in biomass, enzymatic activity and protein concentration in roots and leaves of green bean plants (Phaseolusvulgaris L. cv. Strike) under high NH4NO3 application rates. Sci Hortic 99:237–248CrossRefGoogle Scholar
  25. Scheible W-R, Lauerer M, Schulze E-D, Caboche M, Stitt M (1997) Accumulation of nitrate in the shoot acts as a signal to regulate shoot-root allocation in tobacco. Plant J 11:671–691CrossRefGoogle Scholar
  26. Tanou G, Molassiotis A, Diamantidis G (2009) Induction of reactive oxygen species and necrotic death-like destruction in strawberry leaves by salinity. Environ Exp Bot 65:270–281CrossRefGoogle Scholar
  27. Tong YW, Chen DF (1991) Study on the cause and control of secondary saline soils in greenhouses. Acta Hortic Sin 18:159–162Google Scholar
  28. Veljovic-Jovanovic S, Kukavica B, Stevanovic B, Navari-Izzo F (2006) Senescence-and drought-related changes in peroxidase and superoxide dismutase isoforms in leaves of Ramonda serbica. J Exp Bot 57:1759–1768CrossRefPubMedGoogle Scholar
  29. Wei GP, Yang LF, Zhu YL, Chen G (2009) Changes in oxidative damage, antioxidant enzyme activities and polyamine contents in leaves of grafted and non-grafted eggplant seedlings under stress by excess of calcium nitrate. Sci Hortic 120:443–451CrossRefGoogle Scholar
  30. Yu BJ, Li SN, Liu YL (2002) Comparison of ion effects of salt injury in soybean seedlings. J Nanjing Agric Univ 25:5–9Google Scholar
  31. Yuan LY, Du J, Yuan YH, Shu S, Sun J, Guo SR (2013) Effects of 24-epibrassinolide on ascorbate-glutathione cycle and polyamine levels in cucumber roots under Ca(NO3)2 stress. Acta Physiol Plant 35:253–262CrossRefGoogle Scholar
  32. Yuan LY, Shu S, Sun J, Guo SR, Tezuka T (2012) Effects of 24-epibrassinolide on the photosynthetic characteristics, antioxidant system, and chloroplast ultrastructure in Cucumis sativus L. under Ca(NO3)2 stress. Photosyn Res 112:205–214CrossRefPubMedGoogle Scholar
  33. Zhang GW, Liu ZL, Zhou JG, Zhu YL (2008) Effects of Ca(NO3)2 stress on oxidative damage, antioxidant enzymes activities and polyamine contents in roots of grafted and non-grafted tomato plants. Plant Growth Regul 56:7–19CrossRefGoogle Scholar
  34. Zheng GC (1979) Microscopic technology of biology. Popular Education Press, Beijing, China, p 110Google Scholar

Copyright information

© Korean Society for Horticultural Science and Springer-Verlag GmbH Germany, part of Springer Nature 2017

Authors and Affiliations

  1. 1.The Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, School of Agriculture and Food ScienceZhejiang Agriculture & Forestry UniversityLin’anP. R. China

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