Abstract
‘Jingbaili’ pear (Pyrus ussuriensis Maxim.) fruit shows rapid softening, yellowing and short shelf-life at ambient temperature storage. In this study, ‘Jingbaili’ pear fruits were treated with 1.0 μL·L−1 1-methylcyclopropene (1-MCP) for 24 hours at 25 ± 2°C and then stored at 0°C. After 60 and 120 days of cold storage at 0°C, pear fruits were removed and stored at 20 ± 2°C to assess their shelf-life. The results indicated that the 1-MCP treatment delayed the decrease in firmness and chlorophyll (a, b, and a+b) content of peel, reduced the rates of respiration and ethylene production, and inhibited the accumulation of hydrogen peroxide (H2O2), which was observed in the untreated fruits during and after cold storage. Less difference was found in soluble solids content (SSC) between the control and 1-MCP-treated fruits during storage. The correlation analysis showed that flesh H2O2 content was negatively related to firmness and also peel H2O2 content to peel chlorophyll a+b content. These results suggested that the 1-MCP treatment could delay the fruit softening and chlorophyll degradation by suppressing the accumulation of H2O2 content during and after cold storage in ‘Jingbaili’ pear.
Similar content being viewed by others
Literature Cited
Apel, K. and H. Hirt. 2004. Reactive oxygen species: Metabolism, oxidative stress, and signal transduction. Annu. Rev. Plant Biol. 55:373–399.
Arnon, D.I. 1949. Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiol. 24:1–15.
Bellincampi, D., N. Dipierro, G. Salvi, F. Cervone, and G. De Lorenzo. 2000. Extracellular H2O2 induced by oligogalacturonides is not involved in the inhibition of the auxin-regulated rolB gene expression in tobacco leaf explants. Plant Physiol. 122:1379–1385.
Blankenship, S.M. and J.M. Dole. 2003. 1-Methylcyclopropene: A review. Postharvest Biol. Technol. 28:1–25.
Bowler, C., M. Van Montagu, and D. Inzé. 1992. Superoxide dismutase and stress tolerance. Annu. Rev. Plant Physiol. Plant Mol. Biol. 43:83–116.
Brennan, T., A. Rychter, and C. Frenkel. 1979. Activity of enzymes involved in the turnover of hydrogen peroxide during fruit senescence. Bot. Gaz. 140:384–388.
Brennan, T. and C. Frenkel. 1977. Involvement of hydrogen peroxide in the regulation of senescence in pear. Plant Physiol. 59:411–416.
Cheng, G., X. Duan, J. Shi, W. Lu, Y. Luo, W. Jiang, and Y. Jiang. 2008. Effects of reactive oxygen species on cellular wall disassembly of banana fruit during ripening. Food Chem. 109:319–324.
Cheng, Y., Y. Dong., H. Yan, W. Ge, C. Shen, J. Guan, L. Liu, and Y. Zhang. 2012. Effect of 1-MCP on chlorophyll degradation pathway-associated genes expression and chloroplast ultrastructure during the peel yellowing of Chinese pear fruits in storage. Food Chem. 135:415–422.
Ding, Z.S., S.P. Tian, X.L. Zheng, Z.W. Zhou, and Y. Xu. 2007. Responses of reactive metabolism and quality in mango fruit to exogenous oxalic acid or salicylic acid under chilling temperature stress. Physiol. Plant. 130:112–121.
Dong, Y., L.Q. Liu, and J.F. Guan. 2011. Effects of 1-methylcyclopropene on NO content, NOS activity, and H2O2 content in postharvest Suli pears. Agr. Sci. China 10:797–804.
Fu, L., J. Cao, Q. Li, L. Lin, and W. Jiang. 2007. Effect of 1-methylcyclopropene on fruit quality and physiological disorders in Yali pear (Pyrus bretschneideri Rehd.) during storage. Food Sci. Technol. Int. 13:49–54.
Ito, H., Y. Tanaka, H. Tsuji, and A. Tanaka. 1993. Conversion of chlorophyll b to chlorophyll a by isolated cucumber etioplasts. Arch. Biochem. Biophys. 306:148–151.
Ivanov, B. and S. Khorobrykh. 2003. Participation of photosynthetic electron transport in production and scavenging of reactive oxygen species. Antioxid. Redox Sign. 5:43–53.
Jeong, J., D.J. Huber, and S.A. Sargent. 2002. Influence of 1-methylcycolpropene (1-MCP) on ripening and cell-wall matrix polysaccharides of avocado (Persea americana) fruit. Postharvest Biol. Technol. 25:241–256.
Jimenez, A., G. Creissen, B. Kular, J. Firmin, S. Robinson, M. Verhoeyen, and P. Mullineaux. 2002. Changes in oxidative processes and components of the antioxidant system during tomato fruit ripening. Planta 214:751–758.
Koukounaras, A. and E. Sfakiotakis. 2007. Effect of 1-MCP prestorage treatment on ethylene and CO2 production and quality of ‘Hayward’ kiwifruit during shelf-life after short, medium and long term cold storage. Postharvest Biol. Technol. 46:174–180.
Kuckenberg, J., I. Tartachnyk, and G. Noga. 2008. Evaluation of fluorescence and remission techniques for monitoring changes in peel chlorophyll and internal fruit characteristics in sunlit and shade sides of apple fruit during shelf-life. Postharvest Biol. Technol. 48:231–241.
Larigaudère, C., R. Vilaplana, Y. Soria, and I. Recasens. 2004. Oxidative behavior of Blanquilla pears treated with 1-methylcyclopropene during cold storage. J. Sci. Food Agri. 84:1871–1877.
Medlicott, A.P., M. Bhogal, and S.B. Reynolds. 1986. Changes in peel pigmentation during ripening of mango fruit (Mangifera indica var. Tommy Atkins). Annal. Applied Biol. 109:651–656.
Meng, K., Y. Dong, L. Liu, and J. Guan. 2011. Effect of 1-MCP on the content of H2O2 and activities of its relative enzymes in ‘Lvbaoshi’ pear during ripening and senescence. J. Agri. Univ. Hebei 34:27–31. (in Chinese)
Mubarakshina, M., S. Khorobrykh, and B. Ivanov. 2006. Oxygen reduction in chloroplast thylakoids results in production of hydrogen peroxide inside the membrane. Bioch. Biophys. Acta Bioenergetics 1757:1496–1503.
Porat, P., B. Weiss, L. Cohen, A. Daus, R. Goren, and S. Droby. 1999. Effects of ethylene and 1-methylcyclopropene on the post-harvest qualities of ‘Shamuti’ oranges. Postharvest Biol. Technol. 15:155–163.
Purvis, A.C. and C.R. Barmore. 1981. Involvement of ethylene in chlorophyll degradation in peel of citrus fruits. Plant Physiol. 68:854–856.
Scandalios, J.G. 1993. Oxygen stress and superoxide dismutases. Plant Physiol. 101:7–12.
Scheumann, V., S. Schoch, and W. Rüdiger. 1998. Chlorophyll a formation in the chlorophyll b reductase reaction requires reduced ferredoxin. J. Biol. Chem. 273:35102–35108.
Singh, R. and U.N. Dwivedi. 2008. Effect of ethrel and 1-methylcyclopropene (1-MCP) on antioxidants in mango (Mangifera indica var. Dashehari) during fruit ripening. Food Chem. 111:951–956.
Sisler, E.C. and M. Serek. 1997. Inhibitors of ethylene responses in plants at the receptor level: Recent developments. Physiol. Plant. 100:577–582.
Trebitsh, T., E.E. Goldschmidt, and J. Riov. 1993. Ethylene induces de novo synthesis of chlorophyllase, a chlorophyll degrading enzyme, in Citrus fruit peel. Proc. Nati. Acad. Sci. USA 90:9441–9445.
Trinchero, G.D., G.O. Sozzi, F. Covatta, and A.A. Fraschina. 2004. Inhibition of ethylene action by 1-methylcyclopropene extends postharvest life of “Bartlett” pears. Postharvest Biol. Technol. 32:193–204.
Wang, Y. and D. Sugar. 2013. Internal browning disorder and fruit quality in modified atmosphere packed ‘Bartlett’ pears during storage and transit. Postharvest Biol. Technol. 83:72–82.
Watkins, C.B. 2006. The use of 1-methylcyclopropene (1-MCP) on fruits and vegetables. Biotechnol. Adv. 24:389–409.
Watkins, C.B., J.F. Nock, and B.D. Whitaker. 2000. Response of early, mid and late season apple cultivars to postharvest application of 1-methylcyclopropene (1-MCP) under air and controlled atmosphere storage conditions. Postharvest Biol. Technol. 19:17–32.
Wei, J., F. Ma, J. Guan, J. Yuan, and X. Zhu. 2009. Cell wall metabolism and its regulation in harvested Pyrus ussuriensis Maxin. cv. Jingbaili fruit during ripening. Sci. Agricul. Sin. 42:2987–2996. (in Chinese)
Yuan, G., B. Sun, J. Yuan, and Q. Wang. 2010. Effect of 1-methylcyclopropene on shelf life, visual quality, antioxidant enzymes and health-promoting compounds in broccoli florets. Food Chem. 118:774–781.
Zhang, S., X. Ren, and J. Rao. 2005. Effects of nitric oxide on active oxygen metabolism of postharvest tomato fruit. Acta Hort. Sin. 32:818–822. (in Chinese)
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Dong, Y., Liu, L., Zhang, Y. et al. Effects of 1-MCP on softening, yellowing and H2O2 content in post-harvest ‘Jingbaili’ pear fruit during and after cold storage. Hortic. Environ. Biotechnol. 55, 404–409 (2014). https://doi.org/10.1007/s13580-014-0184-5
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s13580-014-0184-5