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Antibacterial activity and protective effect against gastric cancer by Anthriscus sylvestris fractions

Horticulture, Environment, and Biotechnology volume 54, pages 326–330 (2013)Cite this article

Abstract

The aim of this research was to investigate the industrial application of Anthriscus sylvestris. Antibacterial activities of the n-hexane, methylene chloride (MC), ethyl acetate, and butanol fractions of A. sylvestris were tested against Escherichia coli, Staphylococcus aureus, and Helicobacter pylori. The n-hexane and MC fractions showed a stronger antibacterial activity against S. aureus than other fractions, with an inhibition zone greater than 11 mm in disc assays. At 100 μg·mL−1, all fractions extracted from A. sylvestris scavenged more than 85% of 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl radicals (·OH); however, the n-hexane and MC fractions showed the strongest scavenging activity of DPPH and ·OH. In addition, we found that treatment with the n-hexane and MC fractions inhibited the growth of H. pylori and gastric adenocarcinoma cells. Further testing of falcarindiol and deoxypodophyllotoxin from the MC fraction of A. sylvestris revealed the antibacterial effects against E. coli, S. aureus, and H. pylori, suggesting their potential use as antibacterial agents.

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Authors and Affiliations

  1. Department of Food Science and Nutrition, Pusan National University, Busan, 609-735, Korea

    Eun Ju Cho & Ji Myung Choi

  2. Department of Integrative Plant Science, Chung-Ang University, Anseong, 456-756, Korea

    Hye Min Kim, Jongkee Kim & Sanghyun Lee

  3. Department of Forest Resource Conservation, Korea National Arboretum, Pocheon, 487-821, Korea

    Kyung Choi, Jajung Ku & Kwang-Woo Park

Authors
  1. Eun Ju Cho
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  2. Ji Myung Choi
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  3. Hye Min Kim
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  4. Kyung Choi
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  5. Jajung Ku
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  6. Kwang-Woo Park
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  7. Jongkee Kim
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  8. Sanghyun Lee
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Correspondence to Sanghyun Lee.

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Cho, E.J., Choi, J.M., Kim, H.M. et al. Antibacterial activity and protective effect against gastric cancer by Anthriscus sylvestris fractions. Hortic. Environ. Biotechnol. 54, 326–330 (2013). https://doi.org/10.1007/s13580-013-0170-3

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  • DOI: https://doi.org/10.1007/s13580-013-0170-3

Additional key words

  • extraction
  • fractionation
  • isolation
  • purification
  • reflux